Experiment: D-Alanine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5274 and Pf6N2E2_5275 are separated by 205 nucleotides Pf6N2E2_5275 and Pf6N2E2_5276 are separated by 221 nucleotides Pf6N2E2_5276 and Pf6N2E2_5277 are separated by 143 nucleotides Pf6N2E2_5277 and Pf6N2E2_5278 are separated by 54 nucleotides
Pf6N2E2_5274 - FolM Alternative dihydrofolate reductase 1, at 6,066,831 to 6,067,541
_5274
Pf6N2E2_5275 - putative membrane protein, at 6,067,747 to 6,068,307
_5275
Pf6N2E2_5276 - Transcriptional regulator, MerR family, at 6,068,529 to 6,069,488
_5276
Pf6N2E2_5277 - FIG00953110: hypothetical protein, at 6,069,632 to 6,070,237
_5277
Pf6N2E2_5278 - Signal transduction histidine kinase, at 6,070,292 to 6,070,765
_5278
Position (kb)
6068
6069
6070 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6067.541 kb on + strand at 6067.541 kb on + strand at 6067.542 kb on - strand at 6067.542 kb on - strand at 6067.542 kb on - strand at 6067.542 kb on - strand at 6067.542 kb on - strand at 6067.542 kb on - strand at 6067.542 kb on - strand at 6067.563 kb on - strand at 6067.573 kb on + strand at 6067.573 kb on + strand at 6067.573 kb on + strand at 6067.573 kb on + strand at 6067.574 kb on - strand at 6067.574 kb on - strand at 6067.574 kb on - strand at 6067.574 kb on - strand at 6067.576 kb on - strand at 6067.598 kb on + strand at 6067.598 kb on + strand at 6067.598 kb on + strand at 6067.598 kb on + strand at 6067.598 kb on + strand at 6067.598 kb on + strand at 6067.598 kb on + strand at 6067.598 kb on + strand at 6067.598 kb on + strand at 6067.599 kb on - strand at 6067.599 kb on - strand at 6067.599 kb on - strand at 6067.599 kb on - strand at 6067.599 kb on - strand at 6067.599 kb on - strand at 6067.599 kb on - strand at 6067.599 kb on - strand at 6067.599 kb on - strand at 6067.599 kb on - strand at 6067.599 kb on - strand at 6067.599 kb on - strand at 6067.602 kb on + strand at 6067.602 kb on + strand at 6067.602 kb on + strand at 6067.602 kb on + strand at 6067.602 kb on + strand at 6067.602 kb on + strand at 6067.603 kb on - strand at 6067.603 kb on - strand at 6067.603 kb on - strand at 6067.603 kb on - strand at 6067.603 kb on - strand at 6067.661 kb on + strand at 6067.662 kb on - strand at 6067.662 kb on - strand at 6067.662 kb on - strand at 6067.668 kb on + strand at 6067.668 kb on + strand at 6067.668 kb on + strand at 6067.669 kb on - strand at 6067.827 kb on + strand, within Pf6N2E2_5275 at 6067.827 kb on + strand, within Pf6N2E2_5275 at 6067.827 kb on + strand, within Pf6N2E2_5275 at 6067.828 kb on - strand, within Pf6N2E2_5275 at 6067.835 kb on + strand, within Pf6N2E2_5275 at 6067.835 kb on + strand, within Pf6N2E2_5275 at 6067.835 kb on + strand, within Pf6N2E2_5275 at 6067.836 kb on - strand, within Pf6N2E2_5275 at 6067.836 kb on - strand, within Pf6N2E2_5275 at 6067.863 kb on + strand, within Pf6N2E2_5275 at 6067.864 kb on - strand, within Pf6N2E2_5275 at 6067.864 kb on - strand, within Pf6N2E2_5275 at 6068.108 kb on + strand, within Pf6N2E2_5275 at 6068.109 kb on - strand, within Pf6N2E2_5275 at 6068.109 kb on - strand, within Pf6N2E2_5275 at 6068.301 kb on + strand at 6068.308 kb on + strand at 6068.308 kb on + strand at 6068.308 kb on + strand at 6068.308 kb on + strand at 6068.308 kb on + strand at 6068.308 kb on + strand at 6068.308 kb on + strand at 6068.308 kb on + strand at 6068.309 kb on - strand at 6068.309 kb on - strand at 6068.309 kb on - strand at 6068.309 kb on - strand at 6068.309 kb on - strand at 6068.309 kb on - strand at 6068.309 kb on - strand at 6068.321 kb on - strand at 6068.411 kb on + strand at 6068.411 kb on + strand at 6068.411 kb on + strand at 6068.427 kb on + strand at 6068.427 kb on + strand at 6068.452 kb on + strand at 6068.452 kb on + strand at 6068.452 kb on + strand at 6068.493 kb on + strand at 6068.493 kb on + strand at 6068.493 kb on + strand at 6068.494 kb on - strand at 6068.494 kb on - strand at 6068.494 kb on - strand at 6068.536 kb on + strand at 6068.536 kb on + strand at 6068.536 kb on + strand at 6068.536 kb on + strand at 6068.536 kb on + strand at 6068.537 kb on - strand at 6068.928 kb on + strand, within Pf6N2E2_5276 at 6068.949 kb on + strand, within Pf6N2E2_5276 at 6068.949 kb on + strand, within Pf6N2E2_5276 at 6068.949 kb on + strand, within Pf6N2E2_5276 at 6068.949 kb on + strand, within Pf6N2E2_5276 at 6068.949 kb on + strand, within Pf6N2E2_5276 at 6068.949 kb on + strand, within Pf6N2E2_5276 at 6068.949 kb on + strand, within Pf6N2E2_5276 at 6068.949 kb on + strand, within Pf6N2E2_5276 at 6068.949 kb on + strand, within Pf6N2E2_5276 at 6068.950 kb on - strand, within Pf6N2E2_5276 at 6068.950 kb on - strand, within Pf6N2E2_5276 at 6068.950 kb on - strand, within Pf6N2E2_5276 at 6068.950 kb on - strand, within Pf6N2E2_5276 at 6068.950 kb on - strand, within Pf6N2E2_5276 at 6068.950 kb on - strand, within Pf6N2E2_5276 at 6068.950 kb on - strand, within Pf6N2E2_5276 at 6068.950 kb on - strand, within Pf6N2E2_5276 at 6069.001 kb on + strand, within Pf6N2E2_5276 at 6069.001 kb on + strand, within Pf6N2E2_5276 at 6069.001 kb on + strand, within Pf6N2E2_5276 at 6069.001 kb on + strand, within Pf6N2E2_5276 at 6069.001 kb on + strand, within Pf6N2E2_5276 at 6069.002 kb on - strand, within Pf6N2E2_5276 at 6069.002 kb on - strand, within Pf6N2E2_5276 at 6069.002 kb on - strand, within Pf6N2E2_5276 at 6069.002 kb on - strand, within Pf6N2E2_5276 at 6069.002 kb on - strand, within Pf6N2E2_5276 at 6069.105 kb on - strand, within Pf6N2E2_5276 at 6069.105 kb on - strand, within Pf6N2E2_5276 at 6069.482 kb on + strand at 6069.483 kb on - strand at 6069.502 kb on + strand at 6069.502 kb on + strand at 6069.503 kb on - strand at 6069.511 kb on + strand at 6069.511 kb on + strand at 6069.512 kb on - strand at 6069.512 kb on - strand at 6069.512 kb on - strand at 6069.595 kb on + strand at 6069.596 kb on - strand at 6069.596 kb on - strand at 6069.678 kb on + strand at 6069.678 kb on + strand at 6069.678 kb on + strand at 6069.678 kb on + strand at 6069.678 kb on + strand at 6069.678 kb on + strand at 6069.679 kb on - strand at 6069.679 kb on - strand at 6069.679 kb on - strand at 6069.679 kb on - strand at 6069.786 kb on + strand, within Pf6N2E2_5277 at 6069.806 kb on + strand, within Pf6N2E2_5277 at 6069.807 kb on - strand, within Pf6N2E2_5277 at 6069.854 kb on + strand, within Pf6N2E2_5277 at 6069.854 kb on + strand, within Pf6N2E2_5277 at 6069.854 kb on + strand, within Pf6N2E2_5277 at 6069.855 kb on - strand, within Pf6N2E2_5277 at 6069.855 kb on - strand, within Pf6N2E2_5277 at 6069.855 kb on - strand, within Pf6N2E2_5277 at 6069.855 kb on - strand, within Pf6N2E2_5277 at 6069.855 kb on - strand, within Pf6N2E2_5277 at 6069.855 kb on - strand, within Pf6N2E2_5277 at 6069.954 kb on - strand, within Pf6N2E2_5277 at 6069.954 kb on - strand, within Pf6N2E2_5277 at 6069.954 kb on - strand, within Pf6N2E2_5277 at 6069.954 kb on - strand, within Pf6N2E2_5277 at 6069.954 kb on - strand, within Pf6N2E2_5277 at 6069.954 kb on - strand, within Pf6N2E2_5277 at 6069.972 kb on + strand, within Pf6N2E2_5277 at 6069.973 kb on - strand, within Pf6N2E2_5277 at 6069.973 kb on - strand, within Pf6N2E2_5277 at 6070.075 kb on + strand, within Pf6N2E2_5277 at 6070.133 kb on + strand, within Pf6N2E2_5277 at 6070.260 kb on - strand at 6070.260 kb on - strand at 6070.279 kb on + strand at 6070.279 kb on + strand at 6070.280 kb on - strand at 6070.280 kb on - strand at 6070.294 kb on - strand at 6070.294 kb on - strand at 6070.434 kb on + strand, within Pf6N2E2_5278 at 6070.434 kb on + strand, within Pf6N2E2_5278 at 6070.434 kb on + strand, within Pf6N2E2_5278 at 6070.434 kb on + strand, within Pf6N2E2_5278 at 6070.435 kb on - strand, within Pf6N2E2_5278 at 6070.435 kb on - strand, within Pf6N2E2_5278 at 6070.435 kb on - strand, within Pf6N2E2_5278 at 6070.435 kb on - strand, within Pf6N2E2_5278 at 6070.435 kb on - strand, within Pf6N2E2_5278 at 6070.438 kb on + strand, within Pf6N2E2_5278 at 6070.438 kb on + strand, within Pf6N2E2_5278 at 6070.438 kb on + strand, within Pf6N2E2_5278 at 6070.438 kb on + strand, within Pf6N2E2_5278 at 6070.438 kb on + strand, within Pf6N2E2_5278 at 6070.438 kb on + strand, within Pf6N2E2_5278 at 6070.439 kb on - strand, within Pf6N2E2_5278 at 6070.439 kb on - strand, within Pf6N2E2_5278 at 6070.439 kb on - strand, within Pf6N2E2_5278
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (C) remove 6,067,541 + -0.4 6,067,541 + +0.7 6,067,542 - -0.7 6,067,542 - -0.4 6,067,542 - -0.4 6,067,542 - +1.2 6,067,542 - +0.7 6,067,542 - +1.5 6,067,542 - -1.2 6,067,563 - -0.6 6,067,573 + -0.5 6,067,573 + +0.2 6,067,573 + -1.2 6,067,573 + -0.4 6,067,574 - -0.1 6,067,574 - -0.3 6,067,574 - +0.7 6,067,574 - +0.9 6,067,576 - -0.4 6,067,598 + -0.4 6,067,598 + +0.5 6,067,598 + -0.8 6,067,598 + +0.3 6,067,598 + +0.6 6,067,598 + +0.5 6,067,598 + -0.2 6,067,598 + +0.9 6,067,598 + -0.5 6,067,599 - +0.8 6,067,599 - +0.1 6,067,599 - +0.6 6,067,599 - +0.1 6,067,599 - +0.3 6,067,599 - +0.8 6,067,599 - -1.0 6,067,599 - -2.7 6,067,599 - +1.2 6,067,599 - +0.4 6,067,599 - -0.6 6,067,599 - -0.1 6,067,602 + +0.8 6,067,602 + -0.0 6,067,602 + +0.9 6,067,602 + -1.1 6,067,602 + -0.0 6,067,602 + -0.7 6,067,603 - -0.3 6,067,603 - -0.4 6,067,603 - +0.5 6,067,603 - -0.1 6,067,603 - -0.6 6,067,661 + -0.1 6,067,662 - +0.3 6,067,662 - +0.1 6,067,662 - +0.3 6,067,668 + +0.3 6,067,668 + +0.0 6,067,668 + -0.5 6,067,669 - -0.1 6,067,827 + Pf6N2E2_5275 0.14 -0.1 6,067,827 + Pf6N2E2_5275 0.14 -0.2 6,067,827 + Pf6N2E2_5275 0.14 -0.1 6,067,828 - Pf6N2E2_5275 0.14 -0.4 6,067,835 + Pf6N2E2_5275 0.16 +0.2 6,067,835 + Pf6N2E2_5275 0.16 -0.4 6,067,835 + Pf6N2E2_5275 0.16 +0.2 6,067,836 - Pf6N2E2_5275 0.16 +0.3 6,067,836 - Pf6N2E2_5275 0.16 -0.3 6,067,863 + Pf6N2E2_5275 0.21 +0.0 6,067,864 - Pf6N2E2_5275 0.21 +0.3 6,067,864 - Pf6N2E2_5275 0.21 -0.3 6,068,108 + Pf6N2E2_5275 0.64 +2.5 6,068,109 - Pf6N2E2_5275 0.65 +0.5 6,068,109 - Pf6N2E2_5275 0.65 +1.2 6,068,301 + +0.4 6,068,308 + -0.4 6,068,308 + +0.4 6,068,308 + +0.9 6,068,308 + +0.4 6,068,308 + +0.1 6,068,308 + -0.0 6,068,308 + +0.8 6,068,308 + +0.6 6,068,309 - -0.1 6,068,309 - +0.0 6,068,309 - +0.4 6,068,309 - +0.1 6,068,309 - -0.0 6,068,309 - +0.2 6,068,309 - +0.0 6,068,321 - +0.1 6,068,411 + +0.8 6,068,411 + +1.4 6,068,411 + +0.3 6,068,427 + +0.1 6,068,427 + +0.4 6,068,452 + -0.4 6,068,452 + -0.2 6,068,452 + +0.8 6,068,493 + +1.3 6,068,493 + +0.7 6,068,493 + +0.5 6,068,494 - -0.5 6,068,494 - +0.8 6,068,494 - +0.3 6,068,536 + +0.6 6,068,536 + -0.9 6,068,536 + -0.1 6,068,536 + -0.3 6,068,536 + +0.4 6,068,537 - +0.3 6,068,928 + Pf6N2E2_5276 0.42 +0.7 6,068,949 + Pf6N2E2_5276 0.44 -0.3 6,068,949 + Pf6N2E2_5276 0.44 +0.0 6,068,949 + Pf6N2E2_5276 0.44 +0.3 6,068,949 + Pf6N2E2_5276 0.44 +0.4 6,068,949 + Pf6N2E2_5276 0.44 -0.3 6,068,949 + Pf6N2E2_5276 0.44 -1.0 6,068,949 + Pf6N2E2_5276 0.44 +0.9 6,068,949 + Pf6N2E2_5276 0.44 -2.6 6,068,949 + Pf6N2E2_5276 0.44 +0.9 6,068,950 - Pf6N2E2_5276 0.44 -0.2 6,068,950 - Pf6N2E2_5276 0.44 -1.6 6,068,950 - Pf6N2E2_5276 0.44 -0.2 6,068,950 - Pf6N2E2_5276 0.44 +0.7 6,068,950 - Pf6N2E2_5276 0.44 +1.1 6,068,950 - Pf6N2E2_5276 0.44 +0.1 6,068,950 - Pf6N2E2_5276 0.44 +0.1 6,068,950 - Pf6N2E2_5276 0.44 +0.8 6,069,001 + Pf6N2E2_5276 0.49 +0.6 6,069,001 + Pf6N2E2_5276 0.49 -0.3 6,069,001 + Pf6N2E2_5276 0.49 -1.0 6,069,001 + Pf6N2E2_5276 0.49 +0.9 6,069,001 + Pf6N2E2_5276 0.49 +0.8 6,069,002 - Pf6N2E2_5276 0.49 +0.4 6,069,002 - Pf6N2E2_5276 0.49 -0.7 6,069,002 - Pf6N2E2_5276 0.49 -0.3 6,069,002 - Pf6N2E2_5276 0.49 +0.7 6,069,002 - Pf6N2E2_5276 0.49 -0.1 6,069,105 - Pf6N2E2_5276 0.60 +0.4 6,069,105 - Pf6N2E2_5276 0.60 +0.7 6,069,482 + -0.2 6,069,483 - +0.8 6,069,502 + -0.6 6,069,502 + +0.3 6,069,503 - +0.0 6,069,511 + +0.9 6,069,511 + -0.1 6,069,512 - +0.4 6,069,512 - +0.9 6,069,512 - +0.6 6,069,595 + -0.3 6,069,596 - -0.2 6,069,596 - -0.8 6,069,678 + -0.1 6,069,678 + -0.4 6,069,678 + -0.2 6,069,678 + +0.2 6,069,678 + -2.2 6,069,678 + -0.8 6,069,679 - -0.7 6,069,679 - -0.9 6,069,679 - -1.0 6,069,679 - -3.5 6,069,786 + Pf6N2E2_5277 0.25 +0.2 6,069,806 + Pf6N2E2_5277 0.29 +1.4 6,069,807 - Pf6N2E2_5277 0.29 +0.2 6,069,854 + Pf6N2E2_5277 0.37 +0.1 6,069,854 + Pf6N2E2_5277 0.37 -0.9 6,069,854 + Pf6N2E2_5277 0.37 -0.1 6,069,855 - Pf6N2E2_5277 0.37 -0.7 6,069,855 - Pf6N2E2_5277 0.37 -0.2 6,069,855 - Pf6N2E2_5277 0.37 -2.4 6,069,855 - Pf6N2E2_5277 0.37 +0.7 6,069,855 - Pf6N2E2_5277 0.37 -1.8 6,069,855 - Pf6N2E2_5277 0.37 -1.7 6,069,954 - Pf6N2E2_5277 0.53 -1.6 6,069,954 - Pf6N2E2_5277 0.53 -1.1 6,069,954 - Pf6N2E2_5277 0.53 -0.1 6,069,954 - Pf6N2E2_5277 0.53 -1.1 6,069,954 - Pf6N2E2_5277 0.53 +0.2 6,069,954 - Pf6N2E2_5277 0.53 -1.3 6,069,972 + Pf6N2E2_5277 0.56 -0.2 6,069,973 - Pf6N2E2_5277 0.56 -0.2 6,069,973 - Pf6N2E2_5277 0.56 -0.1 6,070,075 + Pf6N2E2_5277 0.73 +1.0 6,070,133 + Pf6N2E2_5277 0.83 +0.8 6,070,260 - +1.2 6,070,260 - +0.3 6,070,279 + +0.5 6,070,279 + +0.3 6,070,280 - -0.3 6,070,280 - +0.4 6,070,294 - -0.7 6,070,294 - +0.7 6,070,434 + Pf6N2E2_5278 0.30 -0.5 6,070,434 + Pf6N2E2_5278 0.30 -0.2 6,070,434 + Pf6N2E2_5278 0.30 -0.8 6,070,434 + Pf6N2E2_5278 0.30 -0.8 6,070,435 - Pf6N2E2_5278 0.30 -0.4 6,070,435 - Pf6N2E2_5278 0.30 -0.2 6,070,435 - Pf6N2E2_5278 0.30 +0.0 6,070,435 - Pf6N2E2_5278 0.30 -0.0 6,070,435 - Pf6N2E2_5278 0.30 +0.7 6,070,438 + Pf6N2E2_5278 0.31 +0.8 6,070,438 + Pf6N2E2_5278 0.31 -0.5 6,070,438 + Pf6N2E2_5278 0.31 -0.8 6,070,438 + Pf6N2E2_5278 0.31 +0.4 6,070,438 + Pf6N2E2_5278 0.31 -0.7 6,070,438 + Pf6N2E2_5278 0.31 -0.5 6,070,439 - Pf6N2E2_5278 0.31 -0.1 6,070,439 - Pf6N2E2_5278 0.31 +0.8 6,070,439 - Pf6N2E2_5278 0.31 +0.8
Or see this region's nucleotide sequence