Experiment: D-Alanine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3274 and Pf6N2E2_3275 are separated by 2 nucleotides Pf6N2E2_3275 and Pf6N2E2_3276 are separated by 28 nucleotides Pf6N2E2_3276 and Pf6N2E2_3277 overlap by 8 nucleotides Pf6N2E2_3277 and Pf6N2E2_3278 are separated by 284 nucleotides
Pf6N2E2_3274 - Manganese superoxide dismutase (EC 1.15.1.1), at 3,777,973 to 3,778,584
_3274
Pf6N2E2_3275 - FOG: TPR repeat, at 3,778,587 to 3,779,027
_3275
Pf6N2E2_3276 - Fumarate hydratase class II (EC 4.2.1.2), at 3,779,056 to 3,780,432
_3276
Pf6N2E2_3277 - FagA protein, at 3,780,425 to 3,780,820
_3277
Pf6N2E2_3278 - TldE protein, part of TldE/TldD proteolytic complex, at 3,781,105 to 3,782,463
_3278
Position (kb)
3779
3780
3781 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3778.064 kb on + strand, within Pf6N2E2_3274 at 3778.064 kb on + strand, within Pf6N2E2_3274 at 3778.065 kb on - strand, within Pf6N2E2_3274 at 3778.073 kb on + strand, within Pf6N2E2_3274 at 3778.074 kb on - strand, within Pf6N2E2_3274 at 3778.074 kb on - strand, within Pf6N2E2_3274 at 3778.074 kb on - strand, within Pf6N2E2_3274 at 3778.074 kb on - strand, within Pf6N2E2_3274 at 3778.076 kb on + strand, within Pf6N2E2_3274 at 3778.076 kb on + strand, within Pf6N2E2_3274 at 3778.077 kb on - strand, within Pf6N2E2_3274 at 3778.077 kb on - strand, within Pf6N2E2_3274 at 3778.113 kb on + strand, within Pf6N2E2_3274 at 3778.481 kb on + strand, within Pf6N2E2_3274 at 3778.481 kb on + strand, within Pf6N2E2_3274 at 3778.481 kb on + strand, within Pf6N2E2_3274 at 3778.482 kb on - strand, within Pf6N2E2_3274 at 3778.482 kb on - strand, within Pf6N2E2_3274 at 3778.482 kb on - strand, within Pf6N2E2_3274 at 3778.482 kb on - strand, within Pf6N2E2_3274 at 3778.482 kb on - strand, within Pf6N2E2_3274 at 3778.502 kb on + strand, within Pf6N2E2_3274 at 3778.550 kb on + strand at 3778.551 kb on - strand at 3778.575 kb on + strand at 3778.575 kb on + strand at 3778.575 kb on + strand at 3778.670 kb on - strand, within Pf6N2E2_3275 at 3778.708 kb on + strand, within Pf6N2E2_3275 at 3778.708 kb on + strand, within Pf6N2E2_3275 at 3778.708 kb on + strand, within Pf6N2E2_3275 at 3778.708 kb on + strand, within Pf6N2E2_3275 at 3778.708 kb on + strand, within Pf6N2E2_3275 at 3778.708 kb on + strand, within Pf6N2E2_3275 at 3778.708 kb on + strand, within Pf6N2E2_3275 at 3778.709 kb on - strand, within Pf6N2E2_3275 at 3778.709 kb on - strand, within Pf6N2E2_3275 at 3778.709 kb on - strand, within Pf6N2E2_3275 at 3778.709 kb on - strand, within Pf6N2E2_3275 at 3778.948 kb on + strand, within Pf6N2E2_3275 at 3778.949 kb on - strand, within Pf6N2E2_3275 at 3778.965 kb on + strand, within Pf6N2E2_3275 at 3778.966 kb on - strand, within Pf6N2E2_3275 at 3779.009 kb on + strand at 3779.009 kb on + strand at 3779.009 kb on + strand at 3779.010 kb on - strand at 3779.010 kb on - strand at 3779.166 kb on - strand at 3779.950 kb on + strand, within Pf6N2E2_3276 at 3779.950 kb on + strand, within Pf6N2E2_3276 at 3779.950 kb on + strand, within Pf6N2E2_3276 at 3779.951 kb on - strand, within Pf6N2E2_3276 at 3779.951 kb on - strand, within Pf6N2E2_3276 at 3780.194 kb on - strand, within Pf6N2E2_3276 at 3780.253 kb on + strand, within Pf6N2E2_3276 at 3780.254 kb on - strand, within Pf6N2E2_3276 at 3780.254 kb on - strand, within Pf6N2E2_3276 at 3780.254 kb on - strand, within Pf6N2E2_3276 at 3780.315 kb on + strand at 3780.315 kb on + strand at 3780.316 kb on - strand at 3780.316 kb on - strand at 3780.316 kb on - strand at 3780.424 kb on - strand at 3780.424 kb on - strand at 3780.424 kb on - strand at 3780.426 kb on + strand at 3780.426 kb on + strand at 3780.426 kb on + strand at 3780.426 kb on + strand at 3780.427 kb on - strand at 3780.427 kb on - strand at 3780.427 kb on - strand at 3780.427 kb on - strand at 3780.427 kb on - strand at 3780.457 kb on + strand at 3780.458 kb on - strand at 3780.458 kb on - strand at 3780.458 kb on - strand at 3780.714 kb on + strand, within Pf6N2E2_3277 at 3780.714 kb on + strand, within Pf6N2E2_3277 at 3780.715 kb on - strand, within Pf6N2E2_3277 at 3780.792 kb on + strand at 3780.792 kb on + strand at 3780.793 kb on - strand at 3780.793 kb on - strand at 3780.793 kb on - strand at 3780.950 kb on + strand at 3781.189 kb on + strand at 3781.216 kb on - strand at 3781.295 kb on + strand, within Pf6N2E2_3278 at 3781.296 kb on - strand, within Pf6N2E2_3278 at 3781.296 kb on - strand, within Pf6N2E2_3278 at 3781.410 kb on + strand, within Pf6N2E2_3278 at 3781.411 kb on - strand, within Pf6N2E2_3278
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (C) remove 3,778,064 + Pf6N2E2_3274 0.15 -1.4 3,778,064 + Pf6N2E2_3274 0.15 -0.5 3,778,065 - Pf6N2E2_3274 0.15 +0.5 3,778,073 + Pf6N2E2_3274 0.16 -0.5 3,778,074 - Pf6N2E2_3274 0.17 +0.4 3,778,074 - Pf6N2E2_3274 0.17 -1.4 3,778,074 - Pf6N2E2_3274 0.17 +0.2 3,778,074 - Pf6N2E2_3274 0.17 -0.3 3,778,076 + Pf6N2E2_3274 0.17 +0.4 3,778,076 + Pf6N2E2_3274 0.17 +1.6 3,778,077 - Pf6N2E2_3274 0.17 +0.8 3,778,077 - Pf6N2E2_3274 0.17 +1.3 3,778,113 + Pf6N2E2_3274 0.23 -0.4 3,778,481 + Pf6N2E2_3274 0.83 +1.1 3,778,481 + Pf6N2E2_3274 0.83 +0.6 3,778,481 + Pf6N2E2_3274 0.83 -0.8 3,778,482 - Pf6N2E2_3274 0.83 +0.2 3,778,482 - Pf6N2E2_3274 0.83 -0.1 3,778,482 - Pf6N2E2_3274 0.83 -0.6 3,778,482 - Pf6N2E2_3274 0.83 +1.0 3,778,482 - Pf6N2E2_3274 0.83 +0.6 3,778,502 + Pf6N2E2_3274 0.86 -0.3 3,778,550 + -0.6 3,778,551 - -0.6 3,778,575 + +0.8 3,778,575 + +0.9 3,778,575 + +0.9 3,778,670 - Pf6N2E2_3275 0.19 -0.7 3,778,708 + Pf6N2E2_3275 0.27 -0.1 3,778,708 + Pf6N2E2_3275 0.27 -0.2 3,778,708 + Pf6N2E2_3275 0.27 -0.4 3,778,708 + Pf6N2E2_3275 0.27 -1.8 3,778,708 + Pf6N2E2_3275 0.27 -0.6 3,778,708 + Pf6N2E2_3275 0.27 +0.1 3,778,708 + Pf6N2E2_3275 0.27 +0.1 3,778,709 - Pf6N2E2_3275 0.28 -0.2 3,778,709 - Pf6N2E2_3275 0.28 -2.9 3,778,709 - Pf6N2E2_3275 0.28 +0.6 3,778,709 - Pf6N2E2_3275 0.28 +0.7 3,778,948 + Pf6N2E2_3275 0.82 +1.4 3,778,949 - Pf6N2E2_3275 0.82 -0.4 3,778,965 + Pf6N2E2_3275 0.86 +0.6 3,778,966 - Pf6N2E2_3275 0.86 +0.0 3,779,009 + -0.0 3,779,009 + +0.4 3,779,009 + +0.2 3,779,010 - -1.1 3,779,010 - +0.4 3,779,166 - -0.8 3,779,950 + Pf6N2E2_3276 0.65 -0.8 3,779,950 + Pf6N2E2_3276 0.65 +0.6 3,779,950 + Pf6N2E2_3276 0.65 +0.6 3,779,951 - Pf6N2E2_3276 0.65 +1.3 3,779,951 - Pf6N2E2_3276 0.65 -0.3 3,780,194 - Pf6N2E2_3276 0.83 +1.0 3,780,253 + Pf6N2E2_3276 0.87 +0.1 3,780,254 - Pf6N2E2_3276 0.87 +0.5 3,780,254 - Pf6N2E2_3276 0.87 +1.3 3,780,254 - Pf6N2E2_3276 0.87 +0.6 3,780,315 + +0.9 3,780,315 + +0.8 3,780,316 - -0.1 3,780,316 - +1.1 3,780,316 - +0.8 3,780,424 - +0.6 3,780,424 - +0.5 3,780,424 - +0.7 3,780,426 + +0.6 3,780,426 + +1.0 3,780,426 + +1.1 3,780,426 + +0.6 3,780,427 - +0.4 3,780,427 - -1.9 3,780,427 - +0.9 3,780,427 - +0.8 3,780,427 - -0.4 3,780,457 + -0.6 3,780,458 - +1.0 3,780,458 - +0.5 3,780,458 - -0.2 3,780,714 + Pf6N2E2_3277 0.73 -0.3 3,780,714 + Pf6N2E2_3277 0.73 +1.0 3,780,715 - Pf6N2E2_3277 0.73 -0.3 3,780,792 + -0.3 3,780,792 + -0.3 3,780,793 - +0.1 3,780,793 - -0.5 3,780,793 - -0.5 3,780,950 + +0.0 3,781,189 + +0.0 3,781,216 - +0.2 3,781,295 + Pf6N2E2_3278 0.14 -0.1 3,781,296 - Pf6N2E2_3278 0.14 +0.1 3,781,296 - Pf6N2E2_3278 0.14 +0.1 3,781,410 + Pf6N2E2_3278 0.22 +0.4 3,781,411 - Pf6N2E2_3278 0.23 +0.6
Or see this region's nucleotide sequence