Experiment: D-Alanine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1381 and Pf6N2E2_1382 are separated by 305 nucleotides Pf6N2E2_1382 and Pf6N2E2_1383 are separated by 408 nucleotides
Pf6N2E2_1381 - Aldehyde dehydrogenase (EC 1.2.1.3), at 1,620,567 to 1,622,087
_1381
Pf6N2E2_1382 - Quino(hemo)protein alcohol dehydrogenase, PQQ-dependent (EC 1.1.99.8), at 1,622,393 to 1,624,168
_1382
Pf6N2E2_1383 - YD repeat protein, at 1,624,577 to 1,625,677
_1383
Position (kb)
1622
1623
1624
1625 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 1621.604 kb on + strand, within Pf6N2E2_1381 at 1621.604 kb on + strand, within Pf6N2E2_1381 at 1621.708 kb on + strand, within Pf6N2E2_1381 at 1621.984 kb on + strand at 1621.984 kb on + strand at 1621.985 kb on - strand at 1622.080 kb on + strand at 1622.080 kb on + strand at 1622.141 kb on + strand at 1622.141 kb on + strand at 1622.142 kb on - strand at 1622.142 kb on - strand at 1622.192 kb on + strand at 1622.246 kb on - strand at 1622.529 kb on + strand at 1622.529 kb on + strand at 1622.529 kb on + strand at 1622.530 kb on - strand at 1622.704 kb on - strand, within Pf6N2E2_1382 at 1622.765 kb on - strand, within Pf6N2E2_1382 at 1622.765 kb on - strand, within Pf6N2E2_1382 at 1622.765 kb on - strand, within Pf6N2E2_1382 at 1622.765 kb on - strand, within Pf6N2E2_1382 at 1622.766 kb on + strand, within Pf6N2E2_1382 at 1622.766 kb on + strand, within Pf6N2E2_1382 at 1622.766 kb on + strand at 1622.766 kb on + strand, within Pf6N2E2_1382 at 1622.767 kb on - strand, within Pf6N2E2_1382 at 1622.767 kb on - strand, within Pf6N2E2_1382 at 1622.767 kb on - strand, within Pf6N2E2_1382 at 1622.767 kb on - strand, within Pf6N2E2_1382 at 1622.767 kb on - strand, within Pf6N2E2_1382 at 1622.826 kb on + strand, within Pf6N2E2_1382 at 1622.877 kb on + strand, within Pf6N2E2_1382 at 1622.877 kb on + strand, within Pf6N2E2_1382 at 1622.878 kb on - strand, within Pf6N2E2_1382 at 1622.878 kb on - strand, within Pf6N2E2_1382 at 1623.006 kb on + strand, within Pf6N2E2_1382 at 1623.006 kb on + strand, within Pf6N2E2_1382 at 1623.007 kb on - strand, within Pf6N2E2_1382 at 1623.105 kb on + strand, within Pf6N2E2_1382 at 1623.105 kb on + strand, within Pf6N2E2_1382 at 1623.106 kb on - strand, within Pf6N2E2_1382 at 1623.165 kb on + strand, within Pf6N2E2_1382 at 1623.166 kb on - strand, within Pf6N2E2_1382 at 1623.166 kb on - strand, within Pf6N2E2_1382 at 1623.198 kb on + strand, within Pf6N2E2_1382 at 1623.199 kb on - strand, within Pf6N2E2_1382 at 1623.199 kb on - strand, within Pf6N2E2_1382 at 1623.282 kb on + strand, within Pf6N2E2_1382 at 1623.283 kb on - strand, within Pf6N2E2_1382 at 1623.283 kb on - strand, within Pf6N2E2_1382 at 1623.367 kb on - strand, within Pf6N2E2_1382 at 1623.370 kb on - strand, within Pf6N2E2_1382 at 1623.477 kb on + strand, within Pf6N2E2_1382 at 1623.484 kb on - strand, within Pf6N2E2_1382 at 1623.543 kb on + strand, within Pf6N2E2_1382 at 1623.544 kb on - strand, within Pf6N2E2_1382 at 1623.811 kb on - strand, within Pf6N2E2_1382 at 1623.861 kb on + strand, within Pf6N2E2_1382 at 1623.861 kb on + strand, within Pf6N2E2_1382 at 1623.862 kb on - strand, within Pf6N2E2_1382 at 1623.877 kb on - strand, within Pf6N2E2_1382 at 1623.877 kb on - strand, within Pf6N2E2_1382 at 1623.877 kb on - strand, within Pf6N2E2_1382 at 1623.877 kb on - strand, within Pf6N2E2_1382 at 1624.002 kb on + strand at 1624.003 kb on - strand at 1624.195 kb on + strand at 1624.288 kb on - strand at 1624.288 kb on - strand at 1624.288 kb on - strand at 1624.288 kb on - strand at 1624.359 kb on - strand at 1624.441 kb on - strand at 1624.441 kb on - strand at 1624.467 kb on - strand at 1624.472 kb on - strand at 1624.505 kb on + strand at 1624.506 kb on - strand at 1624.506 kb on - strand at 1624.660 kb on - strand at 1624.673 kb on - strand at 1624.692 kb on - strand, within Pf6N2E2_1383 at 1624.696 kb on - strand, within Pf6N2E2_1383 at 1624.796 kb on - strand, within Pf6N2E2_1383 at 1624.796 kb on - strand, within Pf6N2E2_1383 at 1624.833 kb on - strand, within Pf6N2E2_1383 at 1624.900 kb on - strand, within Pf6N2E2_1383 at 1625.015 kb on - strand, within Pf6N2E2_1383
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (C) remove 1,621,604 + Pf6N2E2_1381 0.68 -3.4 1,621,604 + Pf6N2E2_1381 0.68 -1.4 1,621,708 + Pf6N2E2_1381 0.75 -2.0 1,621,984 + -3.9 1,621,984 + -3.1 1,621,985 - -4.0 1,622,080 + -3.2 1,622,080 + -2.5 1,622,141 + -3.5 1,622,141 + -2.4 1,622,142 - -5.0 1,622,142 - -1.4 1,622,192 + -4.2 1,622,246 - -0.7 1,622,529 + +2.1 1,622,529 + +3.1 1,622,529 + +2.5 1,622,530 - +2.3 1,622,704 - Pf6N2E2_1382 0.18 +0.8 1,622,765 - Pf6N2E2_1382 0.21 +1.9 1,622,765 - Pf6N2E2_1382 0.21 +2.2 1,622,765 - Pf6N2E2_1382 0.21 +0.3 1,622,765 - Pf6N2E2_1382 0.21 +1.5 1,622,766 + Pf6N2E2_1382 0.21 +2.4 1,622,766 + Pf6N2E2_1382 0.21 +2.0 1,622,766 + +3.0 1,622,766 + Pf6N2E2_1382 0.21 +1.9 1,622,767 - Pf6N2E2_1382 0.21 +2.4 1,622,767 - Pf6N2E2_1382 0.21 +0.6 1,622,767 - Pf6N2E2_1382 0.21 +0.6 1,622,767 - Pf6N2E2_1382 0.21 +1.3 1,622,767 - Pf6N2E2_1382 0.21 +0.1 1,622,826 + Pf6N2E2_1382 0.24 +1.6 1,622,877 + Pf6N2E2_1382 0.27 +1.7 1,622,877 + Pf6N2E2_1382 0.27 +2.5 1,622,878 - Pf6N2E2_1382 0.27 +2.2 1,622,878 - Pf6N2E2_1382 0.27 +0.8 1,623,006 + Pf6N2E2_1382 0.35 +1.7 1,623,006 + Pf6N2E2_1382 0.35 +1.8 1,623,007 - Pf6N2E2_1382 0.35 +2.0 1,623,105 + Pf6N2E2_1382 0.40 +2.2 1,623,105 + Pf6N2E2_1382 0.40 +2.1 1,623,106 - Pf6N2E2_1382 0.40 +2.3 1,623,165 + Pf6N2E2_1382 0.43 +1.9 1,623,166 - Pf6N2E2_1382 0.44 +2.6 1,623,166 - Pf6N2E2_1382 0.44 +1.6 1,623,198 + Pf6N2E2_1382 0.45 +1.9 1,623,199 - Pf6N2E2_1382 0.45 +1.1 1,623,199 - Pf6N2E2_1382 0.45 +1.4 1,623,282 + Pf6N2E2_1382 0.50 +0.1 1,623,283 - Pf6N2E2_1382 0.50 +1.4 1,623,283 - Pf6N2E2_1382 0.50 +2.3 1,623,367 - Pf6N2E2_1382 0.55 +2.7 1,623,370 - Pf6N2E2_1382 0.55 +2.0 1,623,477 + Pf6N2E2_1382 0.61 +2.3 1,623,484 - Pf6N2E2_1382 0.61 +1.9 1,623,543 + Pf6N2E2_1382 0.65 +0.8 1,623,544 - Pf6N2E2_1382 0.65 +2.0 1,623,811 - Pf6N2E2_1382 0.80 +2.1 1,623,861 + Pf6N2E2_1382 0.83 +2.2 1,623,861 + Pf6N2E2_1382 0.83 +2.0 1,623,862 - Pf6N2E2_1382 0.83 +2.2 1,623,877 - Pf6N2E2_1382 0.84 +1.0 1,623,877 - Pf6N2E2_1382 0.84 +3.3 1,623,877 - Pf6N2E2_1382 0.84 +3.0 1,623,877 - Pf6N2E2_1382 0.84 +2.8 1,624,002 + +2.0 1,624,003 - +1.9 1,624,195 + +1.6 1,624,288 - +0.7 1,624,288 - +0.2 1,624,288 - +2.0 1,624,288 - +2.0 1,624,359 - -0.2 1,624,441 - +1.4 1,624,441 - +0.5 1,624,467 - +0.4 1,624,472 - -0.5 1,624,505 + +0.0 1,624,506 - +1.2 1,624,506 - +0.3 1,624,660 - +0.8 1,624,673 - +0.3 1,624,692 - Pf6N2E2_1383 0.10 +0.6 1,624,696 - Pf6N2E2_1383 0.11 +0.4 1,624,796 - Pf6N2E2_1383 0.20 +0.2 1,624,796 - Pf6N2E2_1383 0.20 +0.5 1,624,833 - Pf6N2E2_1383 0.23 +1.3 1,624,900 - Pf6N2E2_1383 0.29 +1.0 1,625,015 - Pf6N2E2_1383 0.40 +0.6
Or see this region's nucleotide sequence