Experiment: D-Alanine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1376 and Pf6N2E2_1377 are separated by 116 nucleotides Pf6N2E2_1377 and Pf6N2E2_1378 are separated by 384 nucleotides
Pf6N2E2_1376 - probable transmembrane protein, at 1,613,200 to 1,614,051
_1376
Pf6N2E2_1377 - Malate:quinone oxidoreductase (EC 1.1.5.4), at 1,614,168 to 1,615,811
_1377
Pf6N2E2_1378 - hypothetical protein, at 1,616,196 to 1,617,383
_1378
Position (kb)
1614
1615
1616 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1613.197 kb on + strand at 1613.197 kb on + strand at 1613.252 kb on + strand at 1613.252 kb on + strand at 1613.531 kb on + strand, within Pf6N2E2_1376 at 1613.531 kb on + strand, within Pf6N2E2_1376 at 1613.532 kb on - strand, within Pf6N2E2_1376 at 1613.532 kb on - strand, within Pf6N2E2_1376 at 1613.535 kb on - strand, within Pf6N2E2_1376 at 1613.535 kb on - strand, within Pf6N2E2_1376 at 1613.590 kb on - strand, within Pf6N2E2_1376 at 1613.854 kb on + strand, within Pf6N2E2_1376 at 1614.023 kb on - strand at 1614.023 kb on - strand at 1614.145 kb on + strand at 1614.146 kb on - strand at 1614.289 kb on + strand at 1614.611 kb on - strand, within Pf6N2E2_1377 at 1614.611 kb on - strand, within Pf6N2E2_1377 at 1614.611 kb on - strand, within Pf6N2E2_1377 at 1614.668 kb on - strand, within Pf6N2E2_1377 at 1614.752 kb on - strand, within Pf6N2E2_1377 at 1615.079 kb on - strand, within Pf6N2E2_1377 at 1615.168 kb on + strand, within Pf6N2E2_1377 at 1615.169 kb on - strand, within Pf6N2E2_1377 at 1615.415 kb on + strand, within Pf6N2E2_1377 at 1615.518 kb on - strand, within Pf6N2E2_1377 at 1615.549 kb on + strand, within Pf6N2E2_1377 at 1615.549 kb on + strand, within Pf6N2E2_1377 at 1615.818 kb on - strand at 1615.928 kb on - strand at 1615.928 kb on - strand at 1615.942 kb on + strand at 1615.942 kb on + strand at 1615.942 kb on + strand at 1615.942 kb on + strand at 1615.942 kb on + strand at 1615.943 kb on - strand at 1615.959 kb on + strand at 1615.959 kb on + strand at 1615.959 kb on + strand at 1615.959 kb on + strand at 1615.959 kb on + strand at 1615.959 kb on + strand at 1615.960 kb on - strand at 1615.960 kb on - strand at 1615.960 kb on - strand at 1615.960 kb on - strand at 1615.960 kb on - strand at 1615.960 kb on - strand at 1615.961 kb on + strand at 1615.961 kb on + strand at 1615.962 kb on - strand at 1615.985 kb on + strand at 1615.986 kb on - strand at 1615.986 kb on - strand at 1616.077 kb on + strand at 1616.077 kb on + strand at 1616.077 kb on + strand at 1616.077 kb on + strand at 1616.077 kb on + strand at 1616.078 kb on - strand at 1616.078 kb on - strand at 1616.078 kb on - strand at 1616.078 kb on - strand at 1616.081 kb on + strand at 1616.154 kb on - strand at 1616.203 kb on + strand at 1616.203 kb on + strand at 1616.204 kb on - strand at 1616.204 kb on - strand at 1616.311 kb on - strand at 1616.329 kb on + strand, within Pf6N2E2_1378 at 1616.330 kb on - strand, within Pf6N2E2_1378 at 1616.330 kb on - strand, within Pf6N2E2_1378 at 1616.330 kb on - strand, within Pf6N2E2_1378 at 1616.330 kb on - strand, within Pf6N2E2_1378 at 1616.334 kb on - strand, within Pf6N2E2_1378 at 1616.371 kb on + strand, within Pf6N2E2_1378 at 1616.389 kb on + strand, within Pf6N2E2_1378 at 1616.405 kb on - strand, within Pf6N2E2_1378 at 1616.428 kb on + strand, within Pf6N2E2_1378 at 1616.429 kb on - strand, within Pf6N2E2_1378 at 1616.497 kb on - strand, within Pf6N2E2_1378 at 1616.764 kb on + strand, within Pf6N2E2_1378 at 1616.764 kb on + strand, within Pf6N2E2_1378 at 1616.765 kb on - strand, within Pf6N2E2_1378 at 1616.765 kb on - strand, within Pf6N2E2_1378
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (C) remove 1,613,197 + -0.3 1,613,197 + +0.6 1,613,252 + +0.3 1,613,252 + +0.6 1,613,531 + Pf6N2E2_1376 0.39 +0.3 1,613,531 + Pf6N2E2_1376 0.39 -1.2 1,613,532 - Pf6N2E2_1376 0.39 -2.1 1,613,532 - Pf6N2E2_1376 0.39 +0.6 1,613,535 - Pf6N2E2_1376 0.39 -1.3 1,613,535 - Pf6N2E2_1376 0.39 -1.2 1,613,590 - Pf6N2E2_1376 0.46 -0.1 1,613,854 + Pf6N2E2_1376 0.77 +0.6 1,614,023 - -1.0 1,614,023 - +1.3 1,614,145 + +0.4 1,614,146 - -1.0 1,614,289 + -0.3 1,614,611 - Pf6N2E2_1377 0.27 +0.3 1,614,611 - Pf6N2E2_1377 0.27 -0.1 1,614,611 - Pf6N2E2_1377 0.27 -0.1 1,614,668 - Pf6N2E2_1377 0.30 +0.2 1,614,752 - Pf6N2E2_1377 0.36 +0.1 1,615,079 - Pf6N2E2_1377 0.55 +0.4 1,615,168 + Pf6N2E2_1377 0.61 +1.0 1,615,169 - Pf6N2E2_1377 0.61 +0.3 1,615,415 + Pf6N2E2_1377 0.76 +1.4 1,615,518 - Pf6N2E2_1377 0.82 -0.3 1,615,549 + Pf6N2E2_1377 0.84 +1.6 1,615,549 + Pf6N2E2_1377 0.84 +0.6 1,615,818 - +1.7 1,615,928 - +1.1 1,615,928 - +0.2 1,615,942 + +0.1 1,615,942 + +1.1 1,615,942 + -0.8 1,615,942 + +2.5 1,615,942 + +0.7 1,615,943 - -1.1 1,615,959 + -0.6 1,615,959 + -0.1 1,615,959 + +0.3 1,615,959 + -1.0 1,615,959 + -0.1 1,615,959 + +0.8 1,615,960 - +0.6 1,615,960 - +1.3 1,615,960 - +0.6 1,615,960 - +0.5 1,615,960 - +1.4 1,615,960 - -0.7 1,615,961 + -0.0 1,615,961 + +1.8 1,615,962 - +1.4 1,615,985 + -0.4 1,615,986 - -0.8 1,615,986 - +0.6 1,616,077 + -0.3 1,616,077 + -0.7 1,616,077 + +0.6 1,616,077 + -1.4 1,616,077 + +1.0 1,616,078 - +0.8 1,616,078 - +0.6 1,616,078 - +0.5 1,616,078 - +0.0 1,616,081 + +1.4 1,616,154 - +0.0 1,616,203 + +0.2 1,616,203 + +1.2 1,616,204 - -0.2 1,616,204 - -0.7 1,616,311 - +1.0 1,616,329 + Pf6N2E2_1378 0.11 +1.4 1,616,330 - Pf6N2E2_1378 0.11 +0.9 1,616,330 - Pf6N2E2_1378 0.11 -1.0 1,616,330 - Pf6N2E2_1378 0.11 +0.3 1,616,330 - Pf6N2E2_1378 0.11 -0.3 1,616,334 - Pf6N2E2_1378 0.12 +0.5 1,616,371 + Pf6N2E2_1378 0.15 +0.9 1,616,389 + Pf6N2E2_1378 0.16 -1.7 1,616,405 - Pf6N2E2_1378 0.18 +0.1 1,616,428 + Pf6N2E2_1378 0.20 +0.3 1,616,429 - Pf6N2E2_1378 0.20 -0.4 1,616,497 - Pf6N2E2_1378 0.25 +0.7 1,616,764 + Pf6N2E2_1378 0.48 +0.6 1,616,764 + Pf6N2E2_1378 0.48 +1.0 1,616,765 - Pf6N2E2_1378 0.48 +0.9 1,616,765 - Pf6N2E2_1378 0.48 +1.4
Or see this region's nucleotide sequence