Experiment: R2A with Fusidic 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS20255 and CA265_RS20260 are separated by 100 nucleotides CA265_RS20260 and CA265_RS20265 overlap by 9 nucleotides CA265_RS20265 and CA265_RS20270 are separated by 331 nucleotides CA265_RS20270 and CA265_RS20275 are separated by 40 nucleotides CA265_RS20275 and CA265_RS20280 are separated by 1 nucleotides
CA265_RS20255: CA265_RS20255 - trans-aconitate 2-methyltransferase, at 5,004,118 to 5,004,924
_RS20255
CA265_RS20260: CA265_RS20260 - tRNA-Arg, at 5,005,025 to 5,005,100
_RS20260
CA265_RS20265: CA265_RS20265 - excinuclease ABC subunit C, at 5,005,092 to 5,005,337
_RS20265
CA265_RS20270: CA265_RS20270 - methionine--tRNA ligase, at 5,005,669 to 5,007,777
_RS20270
CA265_RS20275: CA265_RS20275 - LD-carboxypeptidase, at 5,007,818 to 5,008,717
_RS20275
CA265_RS20280: CA265_RS20280 - DoxX family protein, at 5,008,719 to 5,009,126
_RS20280
Position (kb) 5005
5006
5007
5008 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5004.673 kb on - strand, within CA265_RS20255 at 5004.746 kb on + strand, within CA265_RS20255 at 5004.839 kb on - strand, within CA265_RS20255 at 5004.871 kb on - strand at 5004.894 kb on + strand at 5004.895 kb on - strand at 5004.943 kb on - strand at 5004.943 kb on - strand at 5004.987 kb on + strand at 5004.987 kb on + strand at 5005.014 kb on - strand at 5005.055 kb on - strand, within CA265_RS20260 at 5005.094 kb on - strand at 5005.213 kb on + strand, within CA265_RS20265 at 5005.213 kb on + strand, within CA265_RS20265 at 5005.254 kb on + strand, within CA265_RS20265 at 5005.317 kb on + strand at 5005.318 kb on - strand at 5005.322 kb on - strand at 5005.338 kb on - strand at 5005.338 kb on - strand at 5005.341 kb on + strand at 5005.341 kb on + strand at 5005.521 kb on - strand at 5005.521 kb on - strand at 5005.532 kb on - strand at 5005.541 kb on + strand at 5005.572 kb on - strand at 5005.583 kb on + strand at 5005.605 kb on + strand at 5005.648 kb on + strand at 5005.648 kb on + strand at 5005.662 kb on + strand at 5005.703 kb on - strand at 5005.892 kb on - strand, within CA265_RS20270 at 5005.940 kb on - strand, within CA265_RS20270 at 5005.942 kb on - strand, within CA265_RS20270 at 5005.942 kb on - strand, within CA265_RS20270 at 5005.960 kb on - strand, within CA265_RS20270 at 5007.823 kb on + strand at 5007.853 kb on + strand at 5007.909 kb on + strand, within CA265_RS20275 at 5007.958 kb on - strand, within CA265_RS20275 at 5007.960 kb on - strand, within CA265_RS20275 at 5008.037 kb on - strand, within CA265_RS20275 at 5008.049 kb on - strand, within CA265_RS20275 at 5008.084 kb on + strand, within CA265_RS20275 at 5008.085 kb on - strand, within CA265_RS20275 at 5008.171 kb on - strand, within CA265_RS20275 at 5008.171 kb on - strand, within CA265_RS20275 at 5008.198 kb on - strand, within CA265_RS20275 at 5008.287 kb on - strand, within CA265_RS20275 at 5008.287 kb on - strand, within CA265_RS20275 at 5008.287 kb on - strand, within CA265_RS20275 at 5008.349 kb on - strand, within CA265_RS20275 at 5008.443 kb on - strand, within CA265_RS20275 at 5008.449 kb on + strand, within CA265_RS20275 at 5008.449 kb on + strand, within CA265_RS20275 at 5008.652 kb on - strand at 5008.684 kb on - strand at 5008.713 kb on + strand at 5008.754 kb on + strand at 5008.771 kb on + strand, within CA265_RS20280
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusidic 0.001 mg/ml remove 5,004,673 - CA265_RS20255 0.69 +0.5 5,004,746 + CA265_RS20255 0.78 +1.0 5,004,839 - CA265_RS20255 0.89 -0.4 5,004,871 - -0.6 5,004,894 + -0.3 5,004,895 - -0.6 5,004,943 - +0.5 5,004,943 - +0.9 5,004,987 + +0.9 5,004,987 + -0.7 5,005,014 - -0.3 5,005,055 - CA265_RS20260 0.39 +0.4 5,005,094 - -0.2 5,005,213 + CA265_RS20265 0.49 -1.0 5,005,213 + CA265_RS20265 0.49 -0.0 5,005,254 + CA265_RS20265 0.66 -2.1 5,005,317 + +0.7 5,005,318 - -1.5 5,005,322 - +0.3 5,005,338 - +0.2 5,005,338 - -1.1 5,005,341 + +0.6 5,005,341 + -0.5 5,005,521 - +1.9 5,005,521 - -0.6 5,005,532 - +1.1 5,005,541 + +0.1 5,005,572 - -1.5 5,005,583 + -0.1 5,005,605 + +0.1 5,005,648 + +0.2 5,005,648 + -0.0 5,005,662 + -0.6 5,005,703 - -0.5 5,005,892 - CA265_RS20270 0.11 -0.1 5,005,940 - CA265_RS20270 0.13 +1.7 5,005,942 - CA265_RS20270 0.13 +0.1 5,005,942 - CA265_RS20270 0.13 +0.7 5,005,960 - CA265_RS20270 0.14 +1.0 5,007,823 + -0.0 5,007,853 + -0.7 5,007,909 + CA265_RS20275 0.10 -0.2 5,007,958 - CA265_RS20275 0.16 +0.1 5,007,960 - CA265_RS20275 0.16 +0.4 5,008,037 - CA265_RS20275 0.24 +0.3 5,008,049 - CA265_RS20275 0.26 -0.5 5,008,084 + CA265_RS20275 0.30 +0.4 5,008,085 - CA265_RS20275 0.30 +1.3 5,008,171 - CA265_RS20275 0.39 -0.4 5,008,171 - CA265_RS20275 0.39 +0.3 5,008,198 - CA265_RS20275 0.42 +0.1 5,008,287 - CA265_RS20275 0.52 -0.1 5,008,287 - CA265_RS20275 0.52 -0.1 5,008,287 - CA265_RS20275 0.52 -1.3 5,008,349 - CA265_RS20275 0.59 -0.5 5,008,443 - CA265_RS20275 0.69 -1.0 5,008,449 + CA265_RS20275 0.70 -0.9 5,008,449 + CA265_RS20275 0.70 -0.3 5,008,652 - +0.7 5,008,684 - +0.3 5,008,713 + -0.2 5,008,754 + +0.8 5,008,771 + CA265_RS20280 0.13 +0.2
Or see this region's nucleotide sequence