Experiment: R2A with Fusidic 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS18470 and CA265_RS18475 are separated by 31 nucleotides CA265_RS18475 and CA265_RS18480 overlap by 1 nucleotides CA265_RS18480 and CA265_RS18485 are separated by 20 nucleotides CA265_RS18485 and CA265_RS18490 are separated by 30 nucleotides CA265_RS18490 and CA265_RS18495 overlap by 1 nucleotides CA265_RS18495 and CA265_RS18500 are separated by 40 nucleotides
CA265_RS18470: CA265_RS18470 - aminotransferase, at 4,597,448 to 4,598,701
_RS18470
CA265_RS18475: CA265_RS18475 - hypothetical protein, at 4,598,733 to 4,599,065
_RS18475
CA265_RS18480: CA265_RS18480 - hypothetical protein, at 4,599,065 to 4,599,253
_RS18480
CA265_RS18485: CA265_RS18485 - arginosuccinate lyase (EC 4.3.2.1) (from data) , at 4,599,274 to 4,600,605
_RS18485
CA265_RS18490: CA265_RS18490 - hypothetical protein, at 4,600,636 to 4,600,968
_RS18490
CA265_RS18495: CA265_RS18495 - hypothetical protein, at 4,600,968 to 4,601,189
_RS18495
CA265_RS18500: CA265_RS18500 - N-succinylcitrulline desuccinylase (EC 3.5.1.-) (from data) , at 4,601,230 to 4,602,297
_RS18500
Position (kb)
4599
4600
4601 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4598.307 kb on + strand, within CA265_RS18470 at 4598.348 kb on - strand, within CA265_RS18470 at 4598.373 kb on + strand, within CA265_RS18470 at 4598.374 kb on - strand, within CA265_RS18470 at 4598.378 kb on - strand, within CA265_RS18470 at 4598.382 kb on + strand, within CA265_RS18470 at 4598.412 kb on - strand, within CA265_RS18470 at 4598.416 kb on + strand, within CA265_RS18470 at 4598.420 kb on - strand, within CA265_RS18470 at 4598.430 kb on + strand, within CA265_RS18470 at 4598.527 kb on - strand, within CA265_RS18470 at 4598.592 kb on - strand at 4598.689 kb on - strand at 4598.705 kb on + strand at 4598.705 kb on + strand at 4598.706 kb on - strand at 4598.706 kb on - strand at 4598.706 kb on - strand at 4598.707 kb on + strand at 4598.719 kb on + strand at 4598.737 kb on - strand at 4598.775 kb on - strand, within CA265_RS18475 at 4598.822 kb on + strand, within CA265_RS18475 at 4598.853 kb on + strand, within CA265_RS18475 at 4598.861 kb on + strand, within CA265_RS18475 at 4598.896 kb on - strand, within CA265_RS18475 at 4598.915 kb on - strand, within CA265_RS18475 at 4598.928 kb on + strand, within CA265_RS18475 at 4598.929 kb on - strand, within CA265_RS18475 at 4598.929 kb on - strand, within CA265_RS18475 at 4598.978 kb on - strand, within CA265_RS18475 at 4599.014 kb on - strand, within CA265_RS18475 at 4599.047 kb on - strand at 4599.066 kb on + strand at 4599.071 kb on - strand at 4599.253 kb on + strand at 4599.363 kb on + strand at 4599.394 kb on - strand at 4599.401 kb on - strand at 4599.453 kb on + strand, within CA265_RS18485 at 4599.453 kb on + strand, within CA265_RS18485 at 4599.500 kb on - strand, within CA265_RS18485 at 4599.500 kb on - strand, within CA265_RS18485 at 4599.519 kb on - strand, within CA265_RS18485 at 4599.519 kb on - strand, within CA265_RS18485 at 4599.580 kb on - strand, within CA265_RS18485 at 4599.580 kb on - strand, within CA265_RS18485 at 4599.941 kb on + strand, within CA265_RS18485 at 4599.976 kb on + strand, within CA265_RS18485 at 4600.227 kb on + strand, within CA265_RS18485 at 4600.228 kb on - strand, within CA265_RS18485 at 4600.346 kb on - strand, within CA265_RS18485 at 4600.409 kb on + strand, within CA265_RS18485 at 4600.409 kb on + strand, within CA265_RS18485 at 4600.504 kb on + strand at 4600.505 kb on - strand at 4600.577 kb on - strand at 4600.618 kb on - strand at 4600.688 kb on - strand, within CA265_RS18490 at 4600.693 kb on + strand, within CA265_RS18490 at 4600.702 kb on + strand, within CA265_RS18490 at 4600.723 kb on + strand, within CA265_RS18490 at 4600.734 kb on + strand, within CA265_RS18490 at 4600.785 kb on + strand, within CA265_RS18490 at 4600.824 kb on - strand, within CA265_RS18490 at 4600.928 kb on - strand, within CA265_RS18490 at 4600.929 kb on + strand, within CA265_RS18490 at 4600.929 kb on + strand, within CA265_RS18490 at 4600.930 kb on - strand, within CA265_RS18490 at 4600.945 kb on - strand at 4600.980 kb on - strand at 4600.982 kb on + strand at 4601.036 kb on + strand, within CA265_RS18495 at 4601.060 kb on + strand, within CA265_RS18495 at 4601.206 kb on - strand at 4601.211 kb on - strand at 4601.284 kb on + strand at 4601.293 kb on - strand at 4601.366 kb on - strand, within CA265_RS18500 at 4601.399 kb on + strand, within CA265_RS18500 at 4601.487 kb on + strand, within CA265_RS18500 at 4601.525 kb on - strand, within CA265_RS18500 at 4601.558 kb on - strand, within CA265_RS18500 at 4601.558 kb on - strand, within CA265_RS18500
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusidic 0.001 mg/ml remove 4,598,307 + CA265_RS18470 0.69 +0.6 4,598,348 - CA265_RS18470 0.72 +0.4 4,598,373 + CA265_RS18470 0.74 +0.3 4,598,374 - CA265_RS18470 0.74 -0.5 4,598,378 - CA265_RS18470 0.74 +2.2 4,598,382 + CA265_RS18470 0.74 +0.1 4,598,412 - CA265_RS18470 0.77 +0.1 4,598,416 + CA265_RS18470 0.77 -0.4 4,598,420 - CA265_RS18470 0.78 +0.1 4,598,430 + CA265_RS18470 0.78 +0.5 4,598,527 - CA265_RS18470 0.86 +1.2 4,598,592 - +0.4 4,598,689 - +0.3 4,598,705 + +0.2 4,598,705 + -0.4 4,598,706 - +0.8 4,598,706 - +0.4 4,598,706 - +0.5 4,598,707 + -0.2 4,598,719 + -0.1 4,598,737 - +0.0 4,598,775 - CA265_RS18475 0.13 +1.1 4,598,822 + CA265_RS18475 0.27 +0.1 4,598,853 + CA265_RS18475 0.36 -1.2 4,598,861 + CA265_RS18475 0.38 +0.1 4,598,896 - CA265_RS18475 0.49 -0.8 4,598,915 - CA265_RS18475 0.55 +0.2 4,598,928 + CA265_RS18475 0.59 -0.8 4,598,929 - CA265_RS18475 0.59 +0.2 4,598,929 - CA265_RS18475 0.59 +0.5 4,598,978 - CA265_RS18475 0.74 +0.2 4,599,014 - CA265_RS18475 0.84 -0.2 4,599,047 - -1.8 4,599,066 + +0.7 4,599,071 - -0.2 4,599,253 + -1.4 4,599,363 + +0.2 4,599,394 - +1.5 4,599,401 - +0.2 4,599,453 + CA265_RS18485 0.13 +0.2 4,599,453 + CA265_RS18485 0.13 +0.6 4,599,500 - CA265_RS18485 0.17 +0.5 4,599,500 - CA265_RS18485 0.17 -1.8 4,599,519 - CA265_RS18485 0.18 +1.5 4,599,519 - CA265_RS18485 0.18 +1.5 4,599,580 - CA265_RS18485 0.23 +0.7 4,599,580 - CA265_RS18485 0.23 +1.5 4,599,941 + CA265_RS18485 0.50 +0.6 4,599,976 + CA265_RS18485 0.53 +0.8 4,600,227 + CA265_RS18485 0.72 +0.3 4,600,228 - CA265_RS18485 0.72 +1.1 4,600,346 - CA265_RS18485 0.80 +0.0 4,600,409 + CA265_RS18485 0.85 -0.3 4,600,409 + CA265_RS18485 0.85 +0.3 4,600,504 + -0.3 4,600,505 - +1.5 4,600,577 - +0.9 4,600,618 - +0.4 4,600,688 - CA265_RS18490 0.16 +0.3 4,600,693 + CA265_RS18490 0.17 +0.8 4,600,702 + CA265_RS18490 0.20 +0.3 4,600,723 + CA265_RS18490 0.26 -0.3 4,600,734 + CA265_RS18490 0.29 +0.3 4,600,785 + CA265_RS18490 0.45 -0.1 4,600,824 - CA265_RS18490 0.56 +0.1 4,600,928 - CA265_RS18490 0.88 -0.5 4,600,929 + CA265_RS18490 0.88 +0.1 4,600,929 + CA265_RS18490 0.88 -1.7 4,600,930 - CA265_RS18490 0.88 +1.5 4,600,945 - +0.1 4,600,980 - +0.9 4,600,982 + -1.1 4,601,036 + CA265_RS18495 0.31 +0.6 4,601,060 + CA265_RS18495 0.41 -0.1 4,601,206 - +0.2 4,601,211 - +1.1 4,601,284 + -0.8 4,601,293 - +0.7 4,601,366 - CA265_RS18500 0.13 -0.7 4,601,399 + CA265_RS18500 0.16 +0.3 4,601,487 + CA265_RS18500 0.24 -0.3 4,601,525 - CA265_RS18500 0.28 +0.3 4,601,558 - CA265_RS18500 0.31 -0.8 4,601,558 - CA265_RS18500 0.31 -0.4
Or see this region's nucleotide sequence