Experiment: R2A with Fusidic 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS08760 and CA265_RS08765 are separated by 1 nucleotides CA265_RS08765 and CA265_RS08770 are separated by 75 nucleotides CA265_RS08770 and CA265_RS08775 are separated by 182 nucleotides CA265_RS08775 and CA265_RS08780 are separated by 314 nucleotides
CA265_RS08760: CA265_RS08760 - histidine kinase, at 2,194,121 to 2,196,334
_RS08760
CA265_RS08765: CA265_RS08765 - response regulator, at 2,196,336 to 2,196,755
_RS08765
CA265_RS08770: CA265_RS08770 - hypothetical protein, at 2,196,831 to 2,197,415
_RS08770
CA265_RS08775: CA265_RS08775 - hypothetical protein, at 2,197,598 to 2,197,777
_RS08775
CA265_RS08780: CA265_RS08780 - hypothetical protein, at 2,198,092 to 2,199,177
_RS08780
Position (kb)
2196
2197
2198 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2195.850 kb on + strand, within CA265_RS08760 at 2195.982 kb on + strand, within CA265_RS08760 at 2195.982 kb on + strand, within CA265_RS08760 at 2195.983 kb on - strand, within CA265_RS08760 at 2195.992 kb on - strand, within CA265_RS08760 at 2196.048 kb on + strand, within CA265_RS08760 at 2196.096 kb on - strand, within CA265_RS08760 at 2196.100 kb on - strand, within CA265_RS08760 at 2196.210 kb on + strand at 2196.211 kb on - strand at 2196.291 kb on + strand at 2196.292 kb on - strand at 2196.330 kb on + strand at 2196.333 kb on - strand at 2196.333 kb on - strand at 2196.351 kb on - strand at 2196.351 kb on - strand at 2196.353 kb on - strand at 2196.358 kb on - strand at 2196.420 kb on - strand, within CA265_RS08765 at 2196.430 kb on + strand, within CA265_RS08765 at 2196.569 kb on + strand, within CA265_RS08765 at 2196.595 kb on + strand, within CA265_RS08765 at 2196.608 kb on + strand, within CA265_RS08765 at 2196.617 kb on + strand, within CA265_RS08765 at 2196.632 kb on + strand, within CA265_RS08765 at 2196.644 kb on - strand, within CA265_RS08765 at 2196.667 kb on + strand, within CA265_RS08765 at 2196.668 kb on - strand, within CA265_RS08765 at 2196.706 kb on + strand, within CA265_RS08765 at 2196.743 kb on - strand at 2196.763 kb on + strand at 2196.768 kb on + strand at 2196.830 kb on + strand at 2196.909 kb on - strand, within CA265_RS08770 at 2196.960 kb on + strand, within CA265_RS08770 at 2197.003 kb on + strand, within CA265_RS08770 at 2197.088 kb on - strand, within CA265_RS08770 at 2197.184 kb on + strand, within CA265_RS08770 at 2197.272 kb on + strand, within CA265_RS08770 at 2197.357 kb on + strand at 2197.357 kb on + strand at 2197.413 kb on + strand at 2197.551 kb on - strand at 2197.750 kb on - strand, within CA265_RS08775 at 2197.854 kb on - strand at 2197.953 kb on + strand at 2197.954 kb on - strand at 2198.010 kb on - strand at 2198.061 kb on - strand at 2198.064 kb on + strand at 2198.065 kb on - strand at 2198.067 kb on + strand at 2198.093 kb on + strand at 2198.103 kb on + strand at 2198.164 kb on + strand at 2198.164 kb on + strand at 2198.164 kb on + strand at 2198.164 kb on + strand at 2198.165 kb on - strand at 2198.165 kb on - strand at 2198.186 kb on + strand at 2198.187 kb on - strand at 2198.250 kb on + strand, within CA265_RS08780 at 2198.276 kb on - strand, within CA265_RS08780 at 2198.289 kb on + strand, within CA265_RS08780 at 2198.289 kb on + strand, within CA265_RS08780 at 2198.293 kb on + strand, within CA265_RS08780 at 2198.293 kb on + strand, within CA265_RS08780 at 2198.338 kb on - strand, within CA265_RS08780 at 2198.338 kb on - strand, within CA265_RS08780 at 2198.343 kb on + strand, within CA265_RS08780 at 2198.344 kb on - strand, within CA265_RS08780 at 2198.389 kb on - strand, within CA265_RS08780 at 2198.390 kb on + strand, within CA265_RS08780 at 2198.394 kb on + strand, within CA265_RS08780
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusidic 0.001 mg/ml remove 2,195,850 + CA265_RS08760 0.78 +1.2 2,195,982 + CA265_RS08760 0.84 -0.3 2,195,982 + CA265_RS08760 0.84 -0.4 2,195,983 - CA265_RS08760 0.84 -0.3 2,195,992 - CA265_RS08760 0.85 -0.1 2,196,048 + CA265_RS08760 0.87 -1.9 2,196,096 - CA265_RS08760 0.89 +0.7 2,196,100 - CA265_RS08760 0.89 +0.3 2,196,210 + +2.4 2,196,211 - -0.8 2,196,291 + -0.1 2,196,292 - -0.8 2,196,330 + -0.4 2,196,333 - -0.1 2,196,333 - +0.4 2,196,351 - +0.2 2,196,351 - -0.5 2,196,353 - -0.7 2,196,358 - +0.3 2,196,420 - CA265_RS08765 0.20 -1.5 2,196,430 + CA265_RS08765 0.22 -1.2 2,196,569 + CA265_RS08765 0.55 -0.2 2,196,595 + CA265_RS08765 0.62 -0.7 2,196,608 + CA265_RS08765 0.65 -0.2 2,196,617 + CA265_RS08765 0.67 +0.8 2,196,632 + CA265_RS08765 0.70 +0.5 2,196,644 - CA265_RS08765 0.73 +0.1 2,196,667 + CA265_RS08765 0.79 -1.0 2,196,668 - CA265_RS08765 0.79 -1.0 2,196,706 + CA265_RS08765 0.88 -2.1 2,196,743 - -0.5 2,196,763 + +0.2 2,196,768 + -2.8 2,196,830 + -0.3 2,196,909 - CA265_RS08770 0.13 +0.2 2,196,960 + CA265_RS08770 0.22 +1.6 2,197,003 + CA265_RS08770 0.29 +0.9 2,197,088 - CA265_RS08770 0.44 -0.1 2,197,184 + CA265_RS08770 0.60 +0.5 2,197,272 + CA265_RS08770 0.75 +0.3 2,197,357 + -0.6 2,197,357 + +0.1 2,197,413 + +0.1 2,197,551 - +1.3 2,197,750 - CA265_RS08775 0.84 -0.3 2,197,854 - -0.1 2,197,953 + -0.1 2,197,954 - +0.4 2,198,010 - +0.0 2,198,061 - +0.0 2,198,064 + -0.0 2,198,065 - +0.4 2,198,067 + -1.0 2,198,093 + +0.6 2,198,103 + -0.1 2,198,164 + +1.0 2,198,164 + -0.2 2,198,164 + -0.1 2,198,164 + +0.1 2,198,165 - +0.2 2,198,165 - -1.4 2,198,186 + -0.1 2,198,187 - -0.7 2,198,250 + CA265_RS08780 0.15 -0.5 2,198,276 - CA265_RS08780 0.17 -0.0 2,198,289 + CA265_RS08780 0.18 -1.1 2,198,289 + CA265_RS08780 0.18 +0.3 2,198,293 + CA265_RS08780 0.19 -2.1 2,198,293 + CA265_RS08780 0.19 -0.2 2,198,338 - CA265_RS08780 0.23 -0.4 2,198,338 - CA265_RS08780 0.23 -3.8 2,198,343 + CA265_RS08780 0.23 -0.6 2,198,344 - CA265_RS08780 0.23 +0.7 2,198,389 - CA265_RS08780 0.27 +0.0 2,198,390 + CA265_RS08780 0.27 -0.2 2,198,394 + CA265_RS08780 0.28 -0.5
Or see this region's nucleotide sequence