Experiment: R2A with Fusidic 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS03395 and CA265_RS03400 overlap by 1 nucleotides CA265_RS03400 and CA265_RS03405 are separated by 143 nucleotides CA265_RS03405 and CA265_RS03410 are separated by 175 nucleotides CA265_RS03410 and CA265_RS03415 overlap by 4 nucleotides CA265_RS03415 and CA265_RS03420 are separated by 51 nucleotides
CA265_RS03395: CA265_RS03395 - DEAD/DEAH box helicase, at 823,732 to 825,078
_RS03395
CA265_RS03400: CA265_RS03400 - hypothetical protein, at 825,078 to 825,347
_RS03400
CA265_RS03405: CA265_RS03405 - gliding motility protein GldC, at 825,491 to 825,826
_RS03405
CA265_RS03410: CA265_RS03410 - hypothetical protein, at 826,002 to 826,400
_RS03410
CA265_RS03415: CA265_RS03415 - hypothetical protein, at 826,397 to 826,906
_RS03415
CA265_RS03420: CA265_RS03420 - hypothetical protein, at 826,958 to 828,022
_RS03420
Position (kb) 826
827 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 825.093 kb on - strand at 825.110 kb on + strand, within CA265_RS03400 at 825.111 kb on - strand, within CA265_RS03400 at 825.192 kb on + strand, within CA265_RS03400 at 825.230 kb on + strand, within CA265_RS03400 at 825.267 kb on - strand, within CA265_RS03400 at 825.275 kb on + strand, within CA265_RS03400 at 825.363 kb on - strand at 825.390 kb on + strand at 825.391 kb on - strand at 825.451 kb on + strand at 825.799 kb on + strand at 825.811 kb on + strand at 825.837 kb on - strand at 825.851 kb on - strand at 825.879 kb on - strand at 825.879 kb on - strand at 825.885 kb on + strand at 826.007 kb on - strand at 826.108 kb on - strand, within CA265_RS03410 at 826.108 kb on - strand, within CA265_RS03410 at 826.131 kb on - strand, within CA265_RS03410 at 826.131 kb on - strand, within CA265_RS03410 at 826.137 kb on - strand, within CA265_RS03410 at 826.140 kb on + strand, within CA265_RS03410 at 826.140 kb on + strand, within CA265_RS03410 at 826.270 kb on - strand, within CA265_RS03410 at 826.275 kb on - strand, within CA265_RS03410 at 826.279 kb on - strand, within CA265_RS03410 at 826.343 kb on - strand, within CA265_RS03410 at 826.541 kb on + strand, within CA265_RS03415 at 826.552 kb on - strand, within CA265_RS03415 at 826.552 kb on - strand, within CA265_RS03415 at 826.552 kb on - strand, within CA265_RS03415 at 826.699 kb on - strand, within CA265_RS03415 at 826.703 kb on - strand, within CA265_RS03415 at 826.719 kb on + strand, within CA265_RS03415 at 826.730 kb on - strand, within CA265_RS03415 at 826.741 kb on - strand, within CA265_RS03415 at 826.889 kb on + strand at 826.925 kb on - strand at 827.001 kb on + strand at 827.001 kb on + strand at 827.001 kb on + strand at 827.008 kb on + strand at 827.048 kb on + strand at 827.073 kb on - strand, within CA265_RS03420 at 827.145 kb on + strand, within CA265_RS03420 at 827.145 kb on + strand, within CA265_RS03420 at 827.146 kb on - strand, within CA265_RS03420 at 827.156 kb on + strand, within CA265_RS03420 at 827.157 kb on - strand, within CA265_RS03420 at 827.176 kb on - strand, within CA265_RS03420 at 827.273 kb on - strand, within CA265_RS03420 at 827.281 kb on + strand, within CA265_RS03420 at 827.281 kb on + strand, within CA265_RS03420 at 827.282 kb on - strand, within CA265_RS03420 at 827.295 kb on - strand, within CA265_RS03420 at 827.327 kb on - strand, within CA265_RS03420 at 827.346 kb on + strand, within CA265_RS03420 at 827.355 kb on - strand, within CA265_RS03420 at 827.355 kb on - strand, within CA265_RS03420
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Fusidic 0.001 mg/ml remove 825,093 - +1.3 825,110 + CA265_RS03400 0.12 -0.8 825,111 - CA265_RS03400 0.12 -0.5 825,192 + CA265_RS03400 0.42 -0.6 825,230 + CA265_RS03400 0.56 +0.4 825,267 - CA265_RS03400 0.70 -0.1 825,275 + CA265_RS03400 0.73 -0.0 825,363 - +0.7 825,390 + +0.2 825,391 - -0.8 825,451 + -1.3 825,799 + -0.9 825,811 + -0.9 825,837 - +1.4 825,851 - +0.8 825,879 - -1.3 825,879 - -1.6 825,885 + +0.3 826,007 - +0.9 826,108 - CA265_RS03410 0.27 -0.8 826,108 - CA265_RS03410 0.27 -2.1 826,131 - CA265_RS03410 0.32 -0.4 826,131 - CA265_RS03410 0.32 +0.6 826,137 - CA265_RS03410 0.34 +0.4 826,140 + CA265_RS03410 0.35 -0.3 826,140 + CA265_RS03410 0.35 +2.1 826,270 - CA265_RS03410 0.67 -0.1 826,275 - CA265_RS03410 0.68 -0.3 826,279 - CA265_RS03410 0.69 -0.6 826,343 - CA265_RS03410 0.85 -0.1 826,541 + CA265_RS03415 0.28 -0.2 826,552 - CA265_RS03415 0.30 -1.6 826,552 - CA265_RS03415 0.30 -0.9 826,552 - CA265_RS03415 0.30 +0.1 826,699 - CA265_RS03415 0.59 +0.3 826,703 - CA265_RS03415 0.60 +0.0 826,719 + CA265_RS03415 0.63 -0.4 826,730 - CA265_RS03415 0.65 -0.4 826,741 - CA265_RS03415 0.67 +1.2 826,889 + +0.4 826,925 - -0.2 827,001 + +0.2 827,001 + +0.6 827,001 + +0.6 827,008 + -0.2 827,048 + +1.7 827,073 - CA265_RS03420 0.11 +0.8 827,145 + CA265_RS03420 0.18 -0.1 827,145 + CA265_RS03420 0.18 -0.5 827,146 - CA265_RS03420 0.18 +0.7 827,156 + CA265_RS03420 0.19 -0.4 827,157 - CA265_RS03420 0.19 +0.2 827,176 - CA265_RS03420 0.20 -0.9 827,273 - CA265_RS03420 0.30 -3.2 827,281 + CA265_RS03420 0.30 -0.3 827,281 + CA265_RS03420 0.30 -0.2 827,282 - CA265_RS03420 0.30 -0.3 827,295 - CA265_RS03420 0.32 +0.8 827,327 - CA265_RS03420 0.35 +0.7 827,346 + CA265_RS03420 0.36 +0.2 827,355 - CA265_RS03420 0.37 -0.9 827,355 - CA265_RS03420 0.37 +0.5
Or see this region's nucleotide sequence