Experiment: Cisplatin 0.0025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_375 and Ac3H11_376 are separated by 301 nucleotides Ac3H11_376 and Ac3H11_564 are separated by 16 nucleotides Ac3H11_564 and Ac3H11_377 are separated by 63 nucleotides
Ac3H11_375 - Tartrate dehydrogenase (EC 1.1.1.93) @ Tartrate decarboxylase (EC 4.1.1.73) @ D-malic enzyme (EC 1.1.1.83), at 50,604 to 51,698
_375
Ac3H11_376 - DNA-binding protein HU-beta, at 52,000 to 52,272
_376
Ac3H11_564 - tRNA-Val-TAC, at 52,289 to 52,361
_564
Ac3H11_377 - Peptidyl-prolyl cis-trans isomerase PpiD (EC 5.2.1.8), at 52,425 to 54,353
_377
Position (kb) 51
52
53 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 51.034 kb on - strand, within Ac3H11_375 at 51.057 kb on + strand, within Ac3H11_375 at 51.057 kb on + strand, within Ac3H11_375 at 51.057 kb on + strand, within Ac3H11_375 at 51.057 kb on + strand, within Ac3H11_375 at 51.057 kb on + strand, within Ac3H11_375 at 51.057 kb on + strand, within Ac3H11_375 at 51.057 kb on + strand, within Ac3H11_375 at 51.057 kb on + strand, within Ac3H11_375 at 51.058 kb on - strand, within Ac3H11_375 at 51.058 kb on - strand, within Ac3H11_375 at 51.058 kb on - strand, within Ac3H11_375 at 51.058 kb on - strand, within Ac3H11_375 at 51.058 kb on - strand, within Ac3H11_375 at 51.253 kb on - strand, within Ac3H11_375 at 51.739 kb on + strand at 51.739 kb on + strand at 51.790 kb on + strand at 51.791 kb on - strand at 51.796 kb on + strand at 51.834 kb on + strand at 51.834 kb on + strand at 51.834 kb on + strand at 51.889 kb on - strand at 51.889 kb on - strand at 52.191 kb on + strand, within Ac3H11_376
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.0025 mg/ml remove 51,034 - Ac3H11_375 0.39 +3.1 51,057 + Ac3H11_375 0.41 +1.7 51,057 + Ac3H11_375 0.41 +0.5 51,057 + Ac3H11_375 0.41 -3.4 51,057 + Ac3H11_375 0.41 +0.5 51,057 + Ac3H11_375 0.41 +0.4 51,057 + Ac3H11_375 0.41 -0.1 51,057 + Ac3H11_375 0.41 +1.3 51,057 + Ac3H11_375 0.41 +0.7 51,058 - Ac3H11_375 0.41 +0.8 51,058 - Ac3H11_375 0.41 +1.2 51,058 - Ac3H11_375 0.41 -0.7 51,058 - Ac3H11_375 0.41 -1.3 51,058 - Ac3H11_375 0.41 +0.4 51,253 - Ac3H11_375 0.59 +0.5 51,739 + +0.5 51,739 + +0.6 51,790 + +2.0 51,791 - -0.5 51,796 + -2.1 51,834 + +1.7 51,834 + +2.6 51,834 + +2.8 51,889 - -1.3 51,889 - -2.5 52,191 + Ac3H11_376 0.70 -2.2
Or see this region's nucleotide sequence