Experiment: Cisplatin 0.0025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_3729 and Ac3H11_3730 are separated by 23 nucleotides Ac3H11_3730 and Ac3H11_3731 are separated by 25 nucleotides Ac3H11_3731 and Ac3H11_3732 are separated by 190 nucleotides Ac3H11_3732 and Ac3H11_3733 are separated by 51 nucleotides
Ac3H11_3729 - Cytosine deaminase (EC 3.5.4.1), at 88,802 to 90,130
_3729
Ac3H11_3730 - Glutathione-regulated potassium-efflux system ancillary protein KefG, at 90,154 to 90,741
_3730
Ac3H11_3731 - Flavodoxin reductases (ferredoxin-NADPH reductases) family 1; Vanillate O-demethylase oxidoreductase (EC 1.14.13.-), at 90,767 to 91,069
_3731
Ac3H11_3732 - Transcriptional regulator RutR of pyrimidine catabolism (TetR family), at 91,260 to 91,934
_3732
Ac3H11_3733 - cytidine/deoxycytidylate deaminase family protein, at 91,986 to 92,561
_3733
Position (kb)
90
91
92 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 90.155 kb on + strand at 90.156 kb on - strand at 90.156 kb on - strand at 90.156 kb on - strand at 90.156 kb on - strand at 90.156 kb on - strand at 90.233 kb on + strand, within Ac3H11_3730 at 90.233 kb on + strand, within Ac3H11_3730 at 90.234 kb on - strand, within Ac3H11_3730 at 90.237 kb on - strand, within Ac3H11_3730 at 90.237 kb on - strand, within Ac3H11_3730 at 90.245 kb on + strand, within Ac3H11_3730 at 90.285 kb on - strand, within Ac3H11_3730 at 90.285 kb on - strand, within Ac3H11_3730 at 90.285 kb on - strand, within Ac3H11_3730 at 90.348 kb on - strand, within Ac3H11_3730 at 90.348 kb on - strand, within Ac3H11_3730 at 90.348 kb on - strand, within Ac3H11_3730 at 90.536 kb on + strand, within Ac3H11_3730 at 90.537 kb on - strand, within Ac3H11_3730 at 90.623 kb on + strand, within Ac3H11_3730 at 90.699 kb on - strand at 90.710 kb on - strand at 90.722 kb on + strand at 90.722 kb on + strand at 90.722 kb on + strand at 90.723 kb on - strand at 90.723 kb on - strand at 91.226 kb on + strand at 91.262 kb on - strand at 91.378 kb on + strand, within Ac3H11_3732 at 91.387 kb on + strand, within Ac3H11_3732 at 91.388 kb on - strand, within Ac3H11_3732 at 91.516 kb on + strand, within Ac3H11_3732 at 91.516 kb on + strand, within Ac3H11_3732 at 91.517 kb on - strand, within Ac3H11_3732 at 91.517 kb on - strand, within Ac3H11_3732 at 91.591 kb on + strand, within Ac3H11_3732 at 91.591 kb on + strand, within Ac3H11_3732 at 91.612 kb on + strand, within Ac3H11_3732 at 91.612 kb on + strand, within Ac3H11_3732 at 91.613 kb on - strand, within Ac3H11_3732 at 91.613 kb on - strand, within Ac3H11_3732 at 91.624 kb on - strand, within Ac3H11_3732 at 91.691 kb on - strand, within Ac3H11_3732 at 91.691 kb on - strand, within Ac3H11_3732 at 91.705 kb on + strand, within Ac3H11_3732 at 91.705 kb on + strand, within Ac3H11_3732 at 91.706 kb on - strand, within Ac3H11_3732 at 91.706 kb on - strand, within Ac3H11_3732 at 91.706 kb on - strand, within Ac3H11_3732 at 91.729 kb on + strand, within Ac3H11_3732 at 91.729 kb on + strand, within Ac3H11_3732 at 91.729 kb on + strand, within Ac3H11_3732 at 91.729 kb on + strand, within Ac3H11_3732 at 91.729 kb on + strand, within Ac3H11_3732 at 91.729 kb on + strand, within Ac3H11_3732 at 91.729 kb on + strand, within Ac3H11_3732 at 91.729 kb on + strand, within Ac3H11_3732 at 91.729 kb on + strand, within Ac3H11_3732 at 91.729 kb on + strand, within Ac3H11_3732 at 91.729 kb on + strand, within Ac3H11_3732 at 91.730 kb on - strand, within Ac3H11_3732 at 91.730 kb on - strand, within Ac3H11_3732 at 91.730 kb on - strand, within Ac3H11_3732 at 91.730 kb on - strand, within Ac3H11_3732 at 91.730 kb on - strand, within Ac3H11_3732 at 91.730 kb on - strand, within Ac3H11_3732 at 91.733 kb on - strand, within Ac3H11_3732 at 91.733 kb on - strand, within Ac3H11_3732
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.0025 mg/ml remove 90,155 + +1.0 90,156 - +0.3 90,156 - +1.3 90,156 - +0.7 90,156 - +2.1 90,156 - +0.3 90,233 + Ac3H11_3730 0.13 +0.3 90,233 + Ac3H11_3730 0.13 +1.1 90,234 - Ac3H11_3730 0.14 -1.0 90,237 - Ac3H11_3730 0.14 -1.6 90,237 - Ac3H11_3730 0.14 -3.2 90,245 + Ac3H11_3730 0.15 +1.1 90,285 - Ac3H11_3730 0.22 -4.2 90,285 - Ac3H11_3730 0.22 -1.6 90,285 - Ac3H11_3730 0.22 +1.9 90,348 - Ac3H11_3730 0.33 -2.1 90,348 - Ac3H11_3730 0.33 -0.7 90,348 - Ac3H11_3730 0.33 +1.7 90,536 + Ac3H11_3730 0.65 -0.3 90,537 - Ac3H11_3730 0.65 -0.0 90,623 + Ac3H11_3730 0.80 -2.2 90,699 - -1.8 90,710 - +0.4 90,722 + -0.9 90,722 + -2.5 90,722 + +0.1 90,723 - +1.0 90,723 - -0.6 91,226 + -1.3 91,262 - +1.5 91,378 + Ac3H11_3732 0.17 -1.2 91,387 + Ac3H11_3732 0.19 -0.7 91,388 - Ac3H11_3732 0.19 -0.5 91,516 + Ac3H11_3732 0.38 -2.4 91,516 + Ac3H11_3732 0.38 +1.0 91,517 - Ac3H11_3732 0.38 -3.2 91,517 - Ac3H11_3732 0.38 -2.3 91,591 + Ac3H11_3732 0.49 -0.2 91,591 + Ac3H11_3732 0.49 -2.0 91,612 + Ac3H11_3732 0.52 +0.9 91,612 + Ac3H11_3732 0.52 +3.9 91,613 - Ac3H11_3732 0.52 +0.4 91,613 - Ac3H11_3732 0.52 -1.6 91,624 - Ac3H11_3732 0.54 +0.6 91,691 - Ac3H11_3732 0.64 +0.1 91,691 - Ac3H11_3732 0.64 +1.0 91,705 + Ac3H11_3732 0.66 -3.1 91,705 + Ac3H11_3732 0.66 +0.9 91,706 - Ac3H11_3732 0.66 +0.5 91,706 - Ac3H11_3732 0.66 +1.1 91,706 - Ac3H11_3732 0.66 -1.5 91,729 + Ac3H11_3732 0.69 +1.7 91,729 + Ac3H11_3732 0.69 -0.3 91,729 + Ac3H11_3732 0.69 -1.6 91,729 + Ac3H11_3732 0.69 +0.5 91,729 + Ac3H11_3732 0.69 -0.4 91,729 + Ac3H11_3732 0.69 +0.6 91,729 + Ac3H11_3732 0.69 +1.7 91,729 + Ac3H11_3732 0.69 -1.6 91,729 + Ac3H11_3732 0.69 +0.2 91,729 + Ac3H11_3732 0.69 -0.8 91,729 + Ac3H11_3732 0.69 +1.1 91,730 - Ac3H11_3732 0.70 -3.0 91,730 - Ac3H11_3732 0.70 +1.2 91,730 - Ac3H11_3732 0.70 -0.3 91,730 - Ac3H11_3732 0.70 -0.9 91,730 - Ac3H11_3732 0.70 -1.3 91,730 - Ac3H11_3732 0.70 -1.2 91,733 - Ac3H11_3732 0.70 +0.6 91,733 - Ac3H11_3732 0.70 +2.1
Or see this region's nucleotide sequence