Experiment: Cisplatin 0.0025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_319 and Ac3H11_320 overlap by 4 nucleotides Ac3H11_320 and Ac3H11_321 overlap by 29 nucleotides Ac3H11_321 and Ac3H11_322 are separated by 54 nucleotides
Ac3H11_318 - Multidrug resistance transporter, Bcr/CflA family, at 142,218 to 143,462
_318
Ac3H11_319 - tRNA dimethylallyltransferase (EC 2.5.1.75), at 143,449 to 144,462
_319
Ac3H11_320 - AttE component of AttEFGH ABC transport system, at 144,459 to 145,112
_320
Ac3H11_321 - hypothetical protein, at 145,084 to 145,290
_321
Ac3H11_322 - Transcriptional regulatory protein, at 145,345 to 146,541
_322
Position (kb) 144
145
146 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 143.603 kb on - strand, within Ac3H11_319 at 143.698 kb on + strand, within Ac3H11_319 at 143.699 kb on - strand, within Ac3H11_319 at 143.794 kb on + strand, within Ac3H11_319 at 143.795 kb on - strand, within Ac3H11_319 at 144.040 kb on - strand, within Ac3H11_319 at 144.495 kb on + strand at 144.495 kb on + strand at 144.495 kb on + strand at 144.495 kb on + strand at 144.617 kb on + strand, within Ac3H11_320 at 144.617 kb on + strand, within Ac3H11_320 at 144.618 kb on - strand, within Ac3H11_320 at 144.830 kb on - strand, within Ac3H11_320 at 145.152 kb on - strand, within Ac3H11_321 at 145.259 kb on + strand, within Ac3H11_321 at 145.259 kb on + strand, within Ac3H11_321 at 145.260 kb on - strand, within Ac3H11_321 at 145.260 kb on - strand, within Ac3H11_321 at 145.260 kb on - strand, within Ac3H11_321 at 145.260 kb on - strand, within Ac3H11_321 at 145.295 kb on - strand at 145.295 kb on - strand at 145.295 kb on - strand at 145.295 kb on - strand at 145.295 kb on - strand at 145.363 kb on + strand at 145.363 kb on + strand at 145.363 kb on + strand at 145.364 kb on - strand at 145.600 kb on + strand, within Ac3H11_322 at 145.600 kb on + strand, within Ac3H11_322 at 145.675 kb on + strand, within Ac3H11_322 at 145.741 kb on + strand, within Ac3H11_322 at 145.741 kb on + strand, within Ac3H11_322 at 145.741 kb on + strand at 145.741 kb on + strand, within Ac3H11_322 at 145.742 kb on - strand, within Ac3H11_322 at 145.742 kb on - strand, within Ac3H11_322 at 145.742 kb on - strand, within Ac3H11_322 at 145.742 kb on - strand, within Ac3H11_322 at 145.742 kb on - strand, within Ac3H11_322 at 145.742 kb on - strand, within Ac3H11_322 at 145.742 kb on - strand, within Ac3H11_322 at 145.742 kb on - strand, within Ac3H11_322 at 145.742 kb on - strand, within Ac3H11_322 at 145.742 kb on - strand, within Ac3H11_322 at 145.822 kb on + strand, within Ac3H11_322 at 145.822 kb on + strand, within Ac3H11_322 at 145.822 kb on + strand, within Ac3H11_322 at 145.940 kb on + strand, within Ac3H11_322 at 145.940 kb on + strand, within Ac3H11_322 at 145.940 kb on + strand, within Ac3H11_322 at 145.940 kb on + strand, within Ac3H11_322 at 145.941 kb on - strand, within Ac3H11_322 at 145.941 kb on - strand, within Ac3H11_322
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.0025 mg/ml remove 143,603 - Ac3H11_319 0.15 +1.5 143,698 + Ac3H11_319 0.25 -0.1 143,699 - Ac3H11_319 0.25 -1.3 143,794 + Ac3H11_319 0.34 -0.5 143,795 - Ac3H11_319 0.34 -0.8 144,040 - Ac3H11_319 0.58 -1.3 144,495 + +1.6 144,495 + +0.9 144,495 + -0.8 144,495 + -0.4 144,617 + Ac3H11_320 0.24 +1.6 144,617 + Ac3H11_320 0.24 -0.7 144,618 - Ac3H11_320 0.24 +1.9 144,830 - Ac3H11_320 0.57 +1.2 145,152 - Ac3H11_321 0.33 +1.1 145,259 + Ac3H11_321 0.85 -2.1 145,259 + Ac3H11_321 0.85 -1.9 145,260 - Ac3H11_321 0.85 -0.2 145,260 - Ac3H11_321 0.85 +0.2 145,260 - Ac3H11_321 0.85 +1.3 145,260 - Ac3H11_321 0.85 +2.3 145,295 - +0.3 145,295 - -2.3 145,295 - +1.4 145,295 - +2.1 145,295 - -2.4 145,363 + -0.1 145,363 + -1.0 145,363 + +0.2 145,364 - -0.5 145,600 + Ac3H11_322 0.21 +4.2 145,600 + Ac3H11_322 0.21 -3.9 145,675 + Ac3H11_322 0.28 -0.7 145,741 + Ac3H11_322 0.33 -0.1 145,741 + Ac3H11_322 0.33 -1.8 145,741 + -0.5 145,741 + Ac3H11_322 0.33 -0.8 145,742 - Ac3H11_322 0.33 +0.5 145,742 - Ac3H11_322 0.33 -0.3 145,742 - Ac3H11_322 0.33 +1.7 145,742 - Ac3H11_322 0.33 -0.0 145,742 - Ac3H11_322 0.33 +0.7 145,742 - Ac3H11_322 0.33 +0.7 145,742 - Ac3H11_322 0.33 -1.6 145,742 - Ac3H11_322 0.33 +0.5 145,742 - Ac3H11_322 0.33 -0.5 145,742 - Ac3H11_322 0.33 -2.5 145,822 + Ac3H11_322 0.40 -1.5 145,822 + Ac3H11_322 0.40 +0.9 145,822 + Ac3H11_322 0.40 -1.3 145,940 + Ac3H11_322 0.50 +0.5 145,940 + Ac3H11_322 0.50 -0.8 145,940 + Ac3H11_322 0.50 +2.0 145,940 + Ac3H11_322 0.50 +1.8 145,941 - Ac3H11_322 0.50 +0.1 145,941 - Ac3H11_322 0.50 +0.6
Or see this region's nucleotide sequence