Experiment: Cisplatin 0.0025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_1935 and Ac3H11_1936 are separated by 7 nucleotides Ac3H11_1936 and Ac3H11_1937 overlap by 4 nucleotides Ac3H11_1937 and Ac3H11_1938 overlap by 4 nucleotides
Ac3H11_1935 - Acetyl-coenzyme A synthetase (EC 6.2.1.1), at 150,353 to 152,209
_1935
Ac3H11_1936 - Branched-chain amino acid transport ATP-binding protein LivF (TC 3.A.1.4.1), at 152,217 to 153,035
_1936
Ac3H11_1937 - Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1), at 153,032 to 153,871
_1937
Ac3H11_1938 - Branched-chain amino acid transport system permease protein LivM (TC 3.A.1.4.1), at 153,868 to 154,944
_1938
Position (kb)
152
153
154 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 151.260 kb on + strand, within Ac3H11_1935 at 151.315 kb on - strand, within Ac3H11_1935 at 151.315 kb on - strand, within Ac3H11_1935 at 151.425 kb on + strand, within Ac3H11_1935 at 151.425 kb on + strand, within Ac3H11_1935 at 151.539 kb on + strand, within Ac3H11_1935 at 151.540 kb on - strand, within Ac3H11_1935 at 151.600 kb on - strand, within Ac3H11_1935 at 151.600 kb on - strand, within Ac3H11_1935 at 151.863 kb on + strand, within Ac3H11_1935 at 151.863 kb on + strand, within Ac3H11_1935 at 151.893 kb on + strand, within Ac3H11_1935 at 151.894 kb on - strand, within Ac3H11_1935 at 152.182 kb on + strand at 152.209 kb on + strand at 152.210 kb on - strand at 152.285 kb on - strand at 152.362 kb on + strand, within Ac3H11_1936 at 152.362 kb on + strand, within Ac3H11_1936 at 152.362 kb on + strand, within Ac3H11_1936 at 152.363 kb on - strand, within Ac3H11_1936 at 152.369 kb on - strand, within Ac3H11_1936 at 152.369 kb on - strand, within Ac3H11_1936 at 152.577 kb on + strand, within Ac3H11_1936 at 152.578 kb on - strand, within Ac3H11_1936 at 152.621 kb on - strand, within Ac3H11_1936 at 152.674 kb on + strand, within Ac3H11_1936 at 152.675 kb on - strand, within Ac3H11_1936 at 152.675 kb on - strand, within Ac3H11_1936 at 152.675 kb on - strand, within Ac3H11_1936 at 152.997 kb on + strand at 153.102 kb on + strand at 153.102 kb on + strand at 153.418 kb on - strand, within Ac3H11_1937 at 153.678 kb on + strand, within Ac3H11_1937 at 153.705 kb on + strand, within Ac3H11_1937 at 153.705 kb on + strand, within Ac3H11_1937 at 153.706 kb on - strand, within Ac3H11_1937 at 153.706 kb on - strand, within Ac3H11_1937 at 154.022 kb on + strand, within Ac3H11_1938 at 154.023 kb on - strand, within Ac3H11_1938 at 154.023 kb on - strand, within Ac3H11_1938 at 154.023 kb on - strand, within Ac3H11_1938 at 154.023 kb on - strand, within Ac3H11_1938
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.0025 mg/ml remove 151,260 + Ac3H11_1935 0.49 -1.3 151,315 - Ac3H11_1935 0.52 -0.8 151,315 - Ac3H11_1935 0.52 -2.7 151,425 + Ac3H11_1935 0.58 -2.3 151,425 + Ac3H11_1935 0.58 +1.4 151,539 + Ac3H11_1935 0.64 +1.3 151,540 - Ac3H11_1935 0.64 -4.0 151,600 - Ac3H11_1935 0.67 +0.9 151,600 - Ac3H11_1935 0.67 +0.8 151,863 + Ac3H11_1935 0.81 -0.8 151,863 + Ac3H11_1935 0.81 +0.0 151,893 + Ac3H11_1935 0.83 -1.8 151,894 - Ac3H11_1935 0.83 -0.8 152,182 + -1.7 152,209 + +1.1 152,210 - -0.0 152,285 - -3.1 152,362 + Ac3H11_1936 0.18 -0.9 152,362 + Ac3H11_1936 0.18 +0.2 152,362 + Ac3H11_1936 0.18 -0.1 152,363 - Ac3H11_1936 0.18 +1.1 152,369 - Ac3H11_1936 0.19 -3.7 152,369 - Ac3H11_1936 0.19 +1.4 152,577 + Ac3H11_1936 0.44 +0.9 152,578 - Ac3H11_1936 0.44 +1.0 152,621 - Ac3H11_1936 0.49 -2.7 152,674 + Ac3H11_1936 0.56 +0.2 152,675 - Ac3H11_1936 0.56 -1.2 152,675 - Ac3H11_1936 0.56 -0.1 152,675 - Ac3H11_1936 0.56 -1.9 152,997 + +1.3 153,102 + +1.0 153,102 + +0.6 153,418 - Ac3H11_1937 0.46 +1.1 153,678 + Ac3H11_1937 0.77 -2.4 153,705 + Ac3H11_1937 0.80 -0.7 153,705 + Ac3H11_1937 0.80 -2.4 153,706 - Ac3H11_1937 0.80 +0.1 153,706 - Ac3H11_1937 0.80 -1.5 154,022 + Ac3H11_1938 0.14 -3.1 154,023 - Ac3H11_1938 0.14 -2.2 154,023 - Ac3H11_1938 0.14 -0.9 154,023 - Ac3H11_1938 0.14 +0.9 154,023 - Ac3H11_1938 0.14 -1.0
Or see this region's nucleotide sequence