Experiment: L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_3415 and Shewana3_3416 are separated by 155 nucleotides Shewana3_3416 and Shewana3_3417 are separated by 137 nucleotides
Shewana3_3415: Shewana3_3415 - hypothetical protein (RefSeq), at 4,085,447 to 4,086,055
_3415
Shewana3_3416: Shewana3_3416 - D-3-phosphoglycerate dehydrogenase (RefSeq), at 4,086,211 to 4,087,440
_3416
Shewana3_3417: Shewana3_3417 - glycine cleavage T protein (aminomethyl transferase) (RefSeq), at 4,087,578 to 4,088,534
_3417
Position (kb)
4086
4087
4088 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1 at 4085.316 kb on + strand at 4085.324 kb on - strand at 4085.445 kb on + strand at 4085.447 kb on - strand at 4085.447 kb on - strand at 4085.453 kb on - strand at 4085.488 kb on - strand at 4085.590 kb on - strand, within Shewana3_3415 at 4085.798 kb on + strand, within Shewana3_3415 at 4085.803 kb on + strand, within Shewana3_3415 at 4085.832 kb on + strand, within Shewana3_3415 at 4085.845 kb on - strand, within Shewana3_3415 at 4086.016 kb on - strand at 4086.150 kb on - strand at 4086.682 kb on + strand, within Shewana3_3416 at 4086.965 kb on + strand, within Shewana3_3416 at 4086.975 kb on + strand, within Shewana3_3416 at 4086.989 kb on - strand, within Shewana3_3416 at 4087.132 kb on - strand, within Shewana3_3416 at 4087.297 kb on + strand, within Shewana3_3416 at 4087.536 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N) remove 4,085,316 + +0.6 4,085,324 - -1.9 4,085,445 + -0.8 4,085,447 - +0.1 4,085,447 - +0.2 4,085,453 - -1.2 4,085,488 - -1.7 4,085,590 - Shewana3_3415 0.23 -1.5 4,085,798 + Shewana3_3415 0.58 +0.3 4,085,803 + Shewana3_3415 0.58 -0.5 4,085,832 + Shewana3_3415 0.63 +1.2 4,085,845 - Shewana3_3415 0.65 -1.2 4,086,016 - -1.6 4,086,150 - -5.9 4,086,682 + Shewana3_3416 0.38 -5.8 4,086,965 + Shewana3_3416 0.61 -6.2 4,086,975 + Shewana3_3416 0.62 -6.8 4,086,989 - Shewana3_3416 0.63 -5.8 4,087,132 - Shewana3_3416 0.75 -4.6 4,087,297 + Shewana3_3416 0.88 -5.8 4,087,536 + -1.1
Or see this region's nucleotide sequence