Experiment: L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2567 and Shewana3_2568 are separated by 145 nucleotides Shewana3_2568 and Shewana3_2569 are separated by 433 nucleotides Shewana3_2569 and rrmA are separated by 306 nucleotides
Shewana3_2567: Shewana3_2567 - RNAse E (RefSeq), at 3,068,272 to 3,071,568
_2567
Shewana3_2568: Shewana3_2568 - sulphate transporter (RefSeq), at 3,071,714 to 3,073,273
_2568
Shewana3_2569: Shewana3_2569 - cold-shock DNA-binding protein family protein (RefSeq), at 3,073,707 to 3,073,916
_2569
Shewana3_2570: rrmA - 23S rRNA methyltransferase A (RefSeq), at 3,074,223 to 3,075,038
rrmA
Position (kb)
3071
3072
3073
3074 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3070.827 kb on + strand, within Shewana3_2567 at 3070.837 kb on + strand, within Shewana3_2567 at 3071.082 kb on + strand, within Shewana3_2567 at 3071.241 kb on + strand at 3071.435 kb on + strand at 3072.479 kb on + strand, within Shewana3_2568 at 3072.740 kb on + strand, within Shewana3_2568 at 3072.936 kb on + strand, within Shewana3_2568 at 3073.081 kb on - strand, within Shewana3_2568 at 3073.473 kb on + strand at 3073.497 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N) remove 3,070,827 + Shewana3_2567 0.77 -3.2 3,070,837 + Shewana3_2567 0.78 -0.7 3,071,082 + Shewana3_2567 0.85 -0.6 3,071,241 + -1.7 3,071,435 + -0.4 3,072,479 + Shewana3_2568 0.49 +0.1 3,072,740 + Shewana3_2568 0.66 +0.3 3,072,936 + Shewana3_2568 0.78 +0.6 3,073,081 - Shewana3_2568 0.88 +1.0 3,073,473 + +0.9 3,073,497 - -0.1
Or see this region's nucleotide sequence