Experiment: L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2120 and Shewana3_2121 are separated by 164 nucleotides Shewana3_2121 and Shewana3_R0089 are separated by 293 nucleotides Shewana3_R0089 and Shewana3_R0090 are separated by 49 nucleotides Shewana3_R0090 and Shewana3_R0091 are separated by 23 nucleotides Shewana3_R0091 and Shewana3_2122 are separated by 325 nucleotides
Shewana3_2120: Shewana3_2120 - hypothetical protein (RefSeq), at 2,543,481 to 2,544,404
_2120
Shewana3_2121: Shewana3_2121 - aromatic amino acid aminotransferase (RefSeq), at 2,544,569 to 2,545,762
_2121
Shewana3_R0089: Shewana3_R0089 - tRNA-Leu (RefSeq), at 2,546,056 to 2,546,142
_R0089
Shewana3_R0090: Shewana3_R0090 - tRNA-Cys (RefSeq), at 2,546,192 to 2,546,265
_R0090
Shewana3_R0091: Shewana3_R0091 - tRNA-Gly (RefSeq), at 2,546,289 to 2,546,364
_R0091
Shewana3_2122: Shewana3_2122 - glyceraldehyde-3-phosphate dehydrogenase (RefSeq), at 2,546,690 to 2,548,123
_2122
Position (kb)
2544
2545
2546 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2543.640 kb on + strand, within Shewana3_2120 at 2543.645 kb on + strand, within Shewana3_2120 at 2543.803 kb on + strand, within Shewana3_2120 at 2544.258 kb on - strand, within Shewana3_2120 at 2544.333 kb on + strand at 2545.073 kb on + strand, within Shewana3_2121 at 2545.081 kb on - strand, within Shewana3_2121 at 2545.137 kb on + strand, within Shewana3_2121 at 2545.403 kb on - strand, within Shewana3_2121 at 2545.606 kb on - strand, within Shewana3_2121 at 2545.649 kb on - strand at 2545.695 kb on + strand at 2545.814 kb on - strand at 2545.826 kb on + strand at 2546.002 kb on - strand at 2546.042 kb on - strand at 2546.614 kb on + strand at 2546.624 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N) remove 2,543,640 + Shewana3_2120 0.17 -1.9 2,543,645 + Shewana3_2120 0.18 +0.5 2,543,803 + Shewana3_2120 0.35 +0.6 2,544,258 - Shewana3_2120 0.84 -2.2 2,544,333 + -1.7 2,545,073 + Shewana3_2121 0.42 -1.2 2,545,081 - Shewana3_2121 0.43 -0.7 2,545,137 + Shewana3_2121 0.48 -2.6 2,545,403 - Shewana3_2121 0.70 -1.3 2,545,606 - Shewana3_2121 0.87 -2.8 2,545,649 - -1.7 2,545,695 + -0.1 2,545,814 - -0.6 2,545,826 + +0.3 2,546,002 - -0.8 2,546,042 - -1.1 2,546,614 + +0.1 2,546,624 + -1.3
Or see this region's nucleotide sequence