Experiment: L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1043 and deoD are separated by 91 nucleotides deoD and Shewana3_1045 are separated by 167 nucleotides Shewana3_1045 and Shewana3_1046 are separated by 79 nucleotides
Shewana3_1043: Shewana3_1043 - phosphopentomutase (RefSeq), at 1,220,286 to 1,221,500
_1043
Shewana3_1044: deoD - purine nucleoside phosphorylase (RefSeq), at 1,221,592 to 1,222,302
deoD
Shewana3_1045: Shewana3_1045 - membrane protein (RefSeq), at 1,222,470 to 1,223,654
_1045
Shewana3_1046: Shewana3_1046 - phosphoserine phosphatase (RefSeq), at 1,223,734 to 1,224,726
_1046
Position (kb)
1222
1223
1224 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 1221.836 kb on + strand, within deoD at 1221.884 kb on + strand, within deoD at 1222.020 kb on + strand, within deoD at 1222.076 kb on - strand at 1222.144 kb on + strand, within deoD at 1222.442 kb on - strand at 1222.569 kb on - strand at 1222.883 kb on - strand, within Shewana3_1045 at 1222.892 kb on - strand, within Shewana3_1045 at 1222.945 kb on + strand, within Shewana3_1045 at 1223.354 kb on + strand, within Shewana3_1045 at 1223.357 kb on - strand, within Shewana3_1045 at 1223.936 kb on - strand, within Shewana3_1046 at 1224.044 kb on + strand, within Shewana3_1046 at 1224.060 kb on - strand, within Shewana3_1046 at 1224.308 kb on - strand, within Shewana3_1046 at 1224.397 kb on - strand, within Shewana3_1046 at 1224.521 kb on - strand, within Shewana3_1046 at 1224.521 kb on - strand, within Shewana3_1046 at 1224.521 kb on - strand, within Shewana3_1046 at 1224.541 kb on + strand, within Shewana3_1046
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N) remove 1,221,836 + deoD Shewana3_1044 0.34 -0.6 1,221,884 + deoD Shewana3_1044 0.41 -0.6 1,222,020 + deoD Shewana3_1044 0.60 -0.2 1,222,076 - -1.5 1,222,144 + deoD Shewana3_1044 0.78 -0.7 1,222,442 - -1.9 1,222,569 - +0.1 1,222,883 - Shewana3_1045 0.35 +0.8 1,222,892 - Shewana3_1045 0.36 +0.4 1,222,945 + Shewana3_1045 0.40 +0.9 1,223,354 + Shewana3_1045 0.75 +1.0 1,223,357 - Shewana3_1045 0.75 +0.1 1,223,936 - Shewana3_1046 0.20 -5.5 1,224,044 + Shewana3_1046 0.31 -5.2 1,224,060 - Shewana3_1046 0.33 -5.2 1,224,308 - Shewana3_1046 0.58 -5.7 1,224,397 - Shewana3_1046 0.67 -2.4 1,224,521 - Shewana3_1046 0.79 -3.9 1,224,521 - Shewana3_1046 0.79 -5.2 1,224,521 - Shewana3_1046 0.79 -6.6 1,224,541 + Shewana3_1046 0.81 -5.5
Or see this region's nucleotide sequence