Experiment: L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0954 and proA are separated by 12 nucleotides proA and Shewana3_0956 are separated by 73 nucleotides Shewana3_0956 and Shewana3_0957 are separated by 124 nucleotides
Shewana3_0954: Shewana3_0954 - gamma-glutamyl kinase (RefSeq), at 1,119,491 to 1,120,609
_0954
Shewana3_0955: proA - gamma-glutamyl phosphate reductase (RefSeq), at 1,120,622 to 1,121,899
proA
Shewana3_0956: Shewana3_0956 - hypothetical protein (RefSeq), at 1,121,973 to 1,122,275
_0956
Shewana3_0957: Shewana3_0957 - ybaK/ebsC protein (RefSeq), at 1,122,400 to 1,122,876
_0957
Position (kb) 1120
1121
1122 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 1119.816 kb on + strand, within Shewana3_0954 at 1119.816 kb on + strand, within Shewana3_0954 at 1119.824 kb on - strand, within Shewana3_0954 at 1119.984 kb on - strand, within Shewana3_0954 at 1120.048 kb on + strand, within Shewana3_0954 at 1120.053 kb on + strand, within Shewana3_0954 at 1120.325 kb on + strand, within Shewana3_0954 at 1120.499 kb on + strand at 1120.580 kb on - strand at 1120.629 kb on + strand at 1120.655 kb on + strand at 1120.696 kb on - strand at 1120.924 kb on - strand, within proA at 1120.949 kb on + strand, within proA at 1120.957 kb on - strand, within proA at 1120.958 kb on - strand, within proA at 1121.122 kb on - strand, within proA at 1121.206 kb on + strand, within proA at 1121.206 kb on + strand, within proA at 1121.360 kb on + strand, within proA at 1121.391 kb on + strand, within proA at 1121.391 kb on + strand, within proA at 1121.399 kb on - strand, within proA at 1121.507 kb on + strand, within proA at 1121.512 kb on + strand, within proA at 1121.561 kb on + strand, within proA at 1121.588 kb on + strand, within proA at 1121.672 kb on - strand, within proA at 1121.856 kb on + strand at 1121.879 kb on + strand at 1122.063 kb on - strand, within Shewana3_0956 at 1122.063 kb on - strand, within Shewana3_0956 at 1122.090 kb on + strand, within Shewana3_0956 at 1122.113 kb on + strand, within Shewana3_0956 at 1122.113 kb on + strand, within Shewana3_0956 at 1122.113 kb on + strand, within Shewana3_0956 at 1122.115 kb on + strand, within Shewana3_0956 at 1122.121 kb on - strand, within Shewana3_0956 at 1122.148 kb on + strand, within Shewana3_0956 at 1122.187 kb on + strand, within Shewana3_0956 at 1122.207 kb on + strand, within Shewana3_0956 at 1122.348 kb on - strand at 1122.414 kb on - strand at 1122.474 kb on - strand, within Shewana3_0957 at 1122.800 kb on - strand, within Shewana3_0957
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N) remove 1,119,816 + Shewana3_0954 0.29 -6.6 1,119,816 + Shewana3_0954 0.29 -5.1 1,119,824 - Shewana3_0954 0.30 -5.3 1,119,984 - Shewana3_0954 0.44 -3.5 1,120,048 + Shewana3_0954 0.50 -2.8 1,120,053 + Shewana3_0954 0.50 -3.8 1,120,325 + Shewana3_0954 0.75 -6.4 1,120,499 + -4.5 1,120,580 - -5.7 1,120,629 + -5.4 1,120,655 + -4.8 1,120,696 - -6.7 1,120,924 - proA Shewana3_0955 0.24 -4.9 1,120,949 + proA Shewana3_0955 0.26 -5.6 1,120,957 - proA Shewana3_0955 0.26 -5.1 1,120,958 - proA Shewana3_0955 0.26 -6.1 1,121,122 - proA Shewana3_0955 0.39 -4.8 1,121,206 + proA Shewana3_0955 0.46 -5.4 1,121,206 + proA Shewana3_0955 0.46 -5.0 1,121,360 + proA Shewana3_0955 0.58 -4.1 1,121,391 + proA Shewana3_0955 0.60 -6.1 1,121,391 + proA Shewana3_0955 0.60 -6.2 1,121,399 - proA Shewana3_0955 0.61 -6.0 1,121,507 + proA Shewana3_0955 0.69 -2.0 1,121,512 + proA Shewana3_0955 0.70 -5.5 1,121,561 + proA Shewana3_0955 0.73 -4.1 1,121,588 + proA Shewana3_0955 0.76 -5.6 1,121,672 - proA Shewana3_0955 0.82 -3.1 1,121,856 + -5.6 1,121,879 + -6.6 1,122,063 - Shewana3_0956 0.30 -1.5 1,122,063 - Shewana3_0956 0.30 +1.0 1,122,090 + Shewana3_0956 0.39 +0.2 1,122,113 + Shewana3_0956 0.46 +0.4 1,122,113 + Shewana3_0956 0.46 +0.3 1,122,113 + Shewana3_0956 0.46 -0.3 1,122,115 + Shewana3_0956 0.47 -1.8 1,122,121 - Shewana3_0956 0.49 +0.3 1,122,148 + Shewana3_0956 0.58 -2.7 1,122,187 + Shewana3_0956 0.71 +0.2 1,122,207 + Shewana3_0956 0.77 -0.5 1,122,348 - +0.3 1,122,414 - +0.3 1,122,474 - Shewana3_0957 0.16 +0.6 1,122,800 - Shewana3_0957 0.84 +0.2
Or see this region's nucleotide sequence