Experiment: L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0702 and rpsR are separated by 11 nucleotides rpsR and rplI are separated by 36 nucleotides rplI and Shewana3_0705 are separated by 157 nucleotides Shewana3_0705 and Shewana3_0706 overlap by 17 nucleotides
Shewana3_0702: Shewana3_0702 - primosomal replication protein N (RefSeq), at 818,705 to 818,995
_0702
Shewana3_0703: rpsR - 30S ribosomal protein S18 (RefSeq), at 819,007 to 819,234
rpsR
Shewana3_0704: rplI - 50S ribosomal protein L9 (RefSeq), at 819,271 to 819,723
rplI
Shewana3_0705: Shewana3_0705 - small GTP-binding protein (RefSeq), at 819,881 to 821,119
_0705
Shewana3_0706: Shewana3_0706 - biotin synthase (RefSeq), at 821,103 to 822,182
_0706
Position (kb) 819
820
821
822 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 819.451 kb on + strand, within rplI at 819.844 kb on + strand at 819.979 kb on - strand at 820.065 kb on - strand, within Shewana3_0705 at 820.271 kb on - strand, within Shewana3_0705 at 820.655 kb on + strand, within Shewana3_0705 at 820.686 kb on + strand, within Shewana3_0705 at 820.700 kb on + strand, within Shewana3_0705 at 820.785 kb on - strand, within Shewana3_0705 at 820.880 kb on + strand, within Shewana3_0705 at 821.011 kb on + strand at 821.109 kb on + strand at 821.144 kb on + strand at 821.144 kb on + strand at 821.144 kb on + strand at 821.170 kb on - strand at 821.402 kb on + strand, within Shewana3_0706 at 821.402 kb on + strand, within Shewana3_0706 at 821.584 kb on - strand, within Shewana3_0706 at 821.841 kb on + strand, within Shewana3_0706 at 821.854 kb on - strand, within Shewana3_0706 at 821.877 kb on - strand, within Shewana3_0706 at 822.083 kb on + strand at 822.102 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N) remove 819,451 + rplI Shewana3_0704 0.40 -3.0 819,844 + +0.6 819,979 - +0.1 820,065 - Shewana3_0705 0.15 -0.1 820,271 - Shewana3_0705 0.31 +0.5 820,655 + Shewana3_0705 0.62 +0.0 820,686 + Shewana3_0705 0.65 +1.2 820,700 + Shewana3_0705 0.66 +0.8 820,785 - Shewana3_0705 0.73 +1.5 820,880 + Shewana3_0705 0.81 -0.1 821,011 + -1.1 821,109 + -0.1 821,144 + +0.0 821,144 + -1.7 821,144 + -1.2 821,170 - -0.1 821,402 + Shewana3_0706 0.28 +0.3 821,402 + Shewana3_0706 0.28 -1.0 821,584 - Shewana3_0706 0.45 +0.4 821,841 + Shewana3_0706 0.68 -1.1 821,854 - Shewana3_0706 0.70 -3.8 821,877 - Shewana3_0706 0.72 -1.5 822,083 + +0.9 822,102 + +0.6
Or see this region's nucleotide sequence