Experiment: L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sspA and Shewana3_0605 overlap by 1 nucleotides Shewana3_0605 and Shewana3_0606 are separated by 155 nucleotides Shewana3_0606 and Shewana3_0607 are separated by 455 nucleotides
Shewana3_0604: sspA - stringent starvation protein A (RefSeq), at 707,522 to 708,151
sspA
Shewana3_0605: Shewana3_0605 - ClpXP protease specificity-enhancing factor (RefSeq), at 708,151 to 708,588
_0605
Shewana3_0606: Shewana3_0606 - aminodeoxychorismate synthase, glutamine amidotransferase subunit (RefSeq), at 708,744 to 709,319
_0606
Shewana3_0607: Shewana3_0607 - peptidase S9 prolyl oligopeptidase (RefSeq), at 709,775 to 712,255
_0607
Position (kb) 708
709
710 Strain fitness (log2 ratio) -2
-1
0
1 at 708.451 kb on + strand, within Shewana3_0605 at 708.603 kb on + strand at 708.678 kb on - strand at 708.810 kb on + strand, within Shewana3_0606 at 708.900 kb on - strand, within Shewana3_0606 at 709.085 kb on + strand, within Shewana3_0606 at 709.343 kb on + strand at 709.351 kb on - strand at 709.499 kb on + strand at 709.757 kb on + strand at 709.886 kb on + strand at 709.926 kb on + strand at 709.926 kb on + strand at 709.926 kb on + strand at 709.948 kb on + strand at 710.075 kb on - strand, within Shewana3_0607 at 710.135 kb on + strand, within Shewana3_0607 at 710.143 kb on - strand, within Shewana3_0607 at 710.153 kb on - strand, within Shewana3_0607 at 710.300 kb on - strand, within Shewana3_0607
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N) remove 708,451 + Shewana3_0605 0.68 -0.1 708,603 + -0.7 708,678 - +0.0 708,810 + Shewana3_0606 0.11 +0.8 708,900 - Shewana3_0606 0.27 -0.8 709,085 + Shewana3_0606 0.59 +0.4 709,343 + +0.9 709,351 - +0.1 709,499 + -2.3 709,757 + +0.4 709,886 + +0.1 709,926 + +1.0 709,926 + +0.1 709,926 + +0.5 709,948 + +0.1 710,075 - Shewana3_0607 0.12 +0.1 710,135 + Shewana3_0607 0.15 -1.2 710,143 - Shewana3_0607 0.15 -1.3 710,153 - Shewana3_0607 0.15 -0.3 710,300 - Shewana3_0607 0.21 +0.4
Or see this region's nucleotide sequence