Experiment: L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0602 and Shewana3_0603 overlap by 1 nucleotides Shewana3_0603 and sspA are separated by 96 nucleotides sspA and Shewana3_0605 overlap by 1 nucleotides Shewana3_0605 and Shewana3_0606 are separated by 155 nucleotides
Shewana3_0602: Shewana3_0602 - cytochrome b/b6 domain-containing protein (RefSeq), at 705,513 to 706,727
_0602
Shewana3_0603: Shewana3_0603 - cytochrome c1 (RefSeq), at 706,727 to 707,425
_0603
Shewana3_0604: sspA - stringent starvation protein A (RefSeq), at 707,522 to 708,151
sspA
Shewana3_0605: Shewana3_0605 - ClpXP protease specificity-enhancing factor (RefSeq), at 708,151 to 708,588
_0605
Shewana3_0606: Shewana3_0606 - aminodeoxychorismate synthase, glutamine amidotransferase subunit (RefSeq), at 708,744 to 709,319
_0606
Position (kb) 707
708
709 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 706.594 kb on + strand, within Shewana3_0602 at 707.094 kb on + strand, within Shewana3_0603 at 707.202 kb on + strand, within Shewana3_0603 at 707.414 kb on + strand at 707.506 kb on + strand at 707.728 kb on + strand, within sspA at 708.451 kb on + strand, within Shewana3_0605 at 708.603 kb on + strand at 708.678 kb on - strand at 708.810 kb on + strand, within Shewana3_0606 at 708.900 kb on - strand, within Shewana3_0606 at 709.085 kb on + strand, within Shewana3_0606
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N) remove 706,594 + Shewana3_0602 0.89 -2.8 707,094 + Shewana3_0603 0.53 -1.7 707,202 + Shewana3_0603 0.68 -2.7 707,414 + -1.3 707,506 + -2.1 707,728 + sspA Shewana3_0604 0.33 -2.7 708,451 + Shewana3_0605 0.68 -0.1 708,603 + -0.7 708,678 - +0.0 708,810 + Shewana3_0606 0.11 +0.8 708,900 - Shewana3_0606 0.27 -0.8 709,085 + Shewana3_0606 0.59 +0.4
Or see this region's nucleotide sequence