Experiment: L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0451 and Shewana3_0452 are separated by 63 nucleotides Shewana3_0452 and Shewana3_0453 are separated by 189 nucleotides Shewana3_0453 and Shewana3_0454 are separated by 164 nucleotides
Shewana3_0451: Shewana3_0451 - anion transporter (RefSeq), at 526,350 to 527,750
_0451
Shewana3_0452: Shewana3_0452 - thioredoxin (RefSeq), at 527,814 to 528,236
_0452
Shewana3_0453: Shewana3_0453 - peptidylprolyl isomerase, FKBP-type (RefSeq), at 528,426 to 528,761
_0453
Shewana3_0454: Shewana3_0454 - diguanylate cyclase with PAS/PAC sensor (RefSeq), at 528,926 to 529,912
_0454
Position (kb) 528
529 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 527.541 kb on + strand, within Shewana3_0451 at 527.549 kb on + strand, within Shewana3_0451 at 527.617 kb on - strand at 527.652 kb on - strand at 527.712 kb on + strand at 527.722 kb on + strand at 527.745 kb on + strand at 527.754 kb on + strand at 527.754 kb on + strand at 527.762 kb on - strand at 527.762 kb on - strand at 527.762 kb on - strand at 527.967 kb on - strand, within Shewana3_0452 at 528.209 kb on - strand at 528.311 kb on + strand at 528.311 kb on + strand at 528.311 kb on + strand at 528.319 kb on - strand at 528.319 kb on - strand at 528.541 kb on - strand, within Shewana3_0453 at 528.663 kb on + strand, within Shewana3_0453 at 528.717 kb on - strand, within Shewana3_0453 at 528.787 kb on + strand at 528.787 kb on + strand at 528.818 kb on - strand at 528.887 kb on - strand at 529.103 kb on - strand, within Shewana3_0454 at 529.103 kb on - strand, within Shewana3_0454 at 529.105 kb on + strand, within Shewana3_0454 at 529.113 kb on - strand, within Shewana3_0454 at 529.113 kb on - strand, within Shewana3_0454 at 529.195 kb on + strand, within Shewana3_0454 at 529.450 kb on + strand, within Shewana3_0454 at 529.505 kb on + strand, within Shewana3_0454 at 529.632 kb on - strand, within Shewana3_0454 at 529.639 kb on + strand, within Shewana3_0454 at 529.688 kb on + strand, within Shewana3_0454 at 529.693 kb on + strand, within Shewana3_0454 at 529.711 kb on - strand, within Shewana3_0454
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N) remove 527,541 + Shewana3_0451 0.85 -1.7 527,549 + Shewana3_0451 0.86 +0.6 527,617 - -0.7 527,652 - +0.4 527,712 + -1.2 527,722 + -1.4 527,745 + -1.5 527,754 + +0.3 527,754 + +0.3 527,762 - +1.9 527,762 - -1.5 527,762 - +0.5 527,967 - Shewana3_0452 0.36 +0.5 528,209 - +0.5 528,311 + -1.0 528,311 + +0.1 528,311 + -1.9 528,319 - -0.8 528,319 - -0.5 528,541 - Shewana3_0453 0.34 +0.4 528,663 + Shewana3_0453 0.71 +0.1 528,717 - Shewana3_0453 0.87 +1.0 528,787 + +0.3 528,787 + +0.6 528,818 - +0.2 528,887 - +0.2 529,103 - Shewana3_0454 0.18 -0.8 529,103 - Shewana3_0454 0.18 -1.1 529,105 + Shewana3_0454 0.18 -2.8 529,113 - Shewana3_0454 0.19 +0.9 529,113 - Shewana3_0454 0.19 -2.3 529,195 + Shewana3_0454 0.27 -1.1 529,450 + Shewana3_0454 0.53 +0.2 529,505 + Shewana3_0454 0.59 +0.2 529,632 - Shewana3_0454 0.72 -1.5 529,639 + Shewana3_0454 0.72 -4.0 529,688 + Shewana3_0454 0.77 -2.1 529,693 + Shewana3_0454 0.78 +0.4 529,711 - Shewana3_0454 0.80 +0.0
Or see this region's nucleotide sequence