Experiment: L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fabG and Shewana3_0328 are separated by 125 nucleotides Shewana3_0328 and Shewana3_0329 are separated by 5 nucleotides Shewana3_0329 and Shewana3_0330 are separated by 96 nucleotides Shewana3_0330 and Shewana3_0331 are separated by 4 nucleotides
Shewana3_0327: fabG - 3-ketoacyl-(acyl-carrier-protein) reductase (RefSeq), at 361,715 to 362,440
fabG
Shewana3_0328: Shewana3_0328 - thioester dehydrase family protein (RefSeq), at 362,566 to 363,105
_0328
Shewana3_0329: Shewana3_0329 - 3-oxoacyl-(acyl carrier protein) synthase I (RefSeq), at 363,111 to 364,328
_0329
Shewana3_0330: Shewana3_0330 - hypothetical protein (RefSeq), at 364,425 to 365,159
_0330
Shewana3_0331: Shewana3_0331 - monooxygenase, FAD-binding (RefSeq), at 365,164 to 366,471
_0331
Position (kb) 363
364
365 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 362.191 kb on - strand, within fabG at 362.260 kb on + strand, within fabG at 362.302 kb on + strand, within fabG at 362.303 kb on - strand, within fabG at 362.310 kb on - strand, within fabG at 362.420 kb on + strand at 362.501 kb on + strand at 362.529 kb on + strand at 362.529 kb on + strand at 362.529 kb on + strand at 362.537 kb on - strand at 362.564 kb on + strand at 362.564 kb on + strand at 362.564 kb on + strand at 362.572 kb on - strand at 362.712 kb on - strand, within Shewana3_0328 at 362.764 kb on - strand, within Shewana3_0328 at 362.764 kb on - strand, within Shewana3_0328 at 362.773 kb on + strand, within Shewana3_0328 at 362.871 kb on - strand, within Shewana3_0328 at 363.171 kb on + strand at 363.298 kb on + strand, within Shewana3_0329 at 363.457 kb on - strand, within Shewana3_0329 at 363.503 kb on + strand, within Shewana3_0329 at 363.567 kb on - strand, within Shewana3_0329 at 363.582 kb on - strand, within Shewana3_0329 at 363.700 kb on + strand, within Shewana3_0329 at 363.708 kb on - strand, within Shewana3_0329 at 363.828 kb on + strand, within Shewana3_0329 at 363.981 kb on + strand, within Shewana3_0329 at 364.015 kb on + strand, within Shewana3_0329 at 364.023 kb on + strand, within Shewana3_0329 at 364.023 kb on + strand, within Shewana3_0329 at 364.056 kb on - strand, within Shewana3_0329 at 364.056 kb on - strand, within Shewana3_0329 at 364.226 kb on - strand at 364.254 kb on + strand at 364.262 kb on - strand at 364.262 kb on - strand at 364.262 kb on - strand at 364.263 kb on - strand at 364.350 kb on + strand at 364.358 kb on - strand at 364.704 kb on - strand, within Shewana3_0330 at 364.773 kb on - strand, within Shewana3_0330 at 364.784 kb on + strand, within Shewana3_0330 at 364.789 kb on + strand, within Shewana3_0330 at 364.789 kb on + strand, within Shewana3_0330 at 364.859 kb on + strand, within Shewana3_0330 at 364.859 kb on + strand, within Shewana3_0330 at 364.859 kb on + strand, within Shewana3_0330 at 364.867 kb on - strand, within Shewana3_0330 at 364.867 kb on - strand, within Shewana3_0330 at 364.867 kb on - strand, within Shewana3_0330 at 365.009 kb on + strand, within Shewana3_0330 at 365.017 kb on - strand, within Shewana3_0330 at 365.147 kb on + strand at 365.155 kb on - strand at 365.155 kb on - strand at 365.155 kb on - strand at 365.155 kb on - strand at 365.162 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N) remove 362,191 - fabG Shewana3_0327 0.66 -0.1 362,260 + fabG Shewana3_0327 0.75 +0.8 362,302 + fabG Shewana3_0327 0.81 +0.9 362,303 - fabG Shewana3_0327 0.81 -0.1 362,310 - fabG Shewana3_0327 0.82 +0.4 362,420 + +0.7 362,501 + +0.4 362,529 + +0.2 362,529 + +0.3 362,529 + +0.3 362,537 - -1.1 362,564 + +0.3 362,564 + -1.5 362,564 + +0.3 362,572 - +0.4 362,712 - Shewana3_0328 0.27 +0.1 362,764 - Shewana3_0328 0.37 -0.5 362,764 - Shewana3_0328 0.37 +0.2 362,773 + Shewana3_0328 0.38 -5.0 362,871 - Shewana3_0328 0.56 -0.7 363,171 + +0.5 363,298 + Shewana3_0329 0.15 +0.3 363,457 - Shewana3_0329 0.28 +0.1 363,503 + Shewana3_0329 0.32 -1.7 363,567 - Shewana3_0329 0.37 -1.2 363,582 - Shewana3_0329 0.39 -0.6 363,700 + Shewana3_0329 0.48 +0.3 363,708 - Shewana3_0329 0.49 +0.3 363,828 + Shewana3_0329 0.59 +0.6 363,981 + Shewana3_0329 0.71 +0.2 364,015 + Shewana3_0329 0.74 -0.9 364,023 + Shewana3_0329 0.75 -1.4 364,023 + Shewana3_0329 0.75 +0.9 364,056 - Shewana3_0329 0.78 +0.7 364,056 - Shewana3_0329 0.78 -1.4 364,226 - -2.0 364,254 + -0.7 364,262 - +0.2 364,262 - -0.1 364,262 - -1.6 364,263 - +0.7 364,350 + +0.2 364,358 - +0.2 364,704 - Shewana3_0330 0.38 -1.5 364,773 - Shewana3_0330 0.47 -2.1 364,784 + Shewana3_0330 0.49 -0.0 364,789 + Shewana3_0330 0.50 +0.5 364,789 + Shewana3_0330 0.50 -0.1 364,859 + Shewana3_0330 0.59 +0.5 364,859 + Shewana3_0330 0.59 -5.7 364,859 + Shewana3_0330 0.59 +0.1 364,867 - Shewana3_0330 0.60 -0.1 364,867 - Shewana3_0330 0.60 -0.5 364,867 - Shewana3_0330 0.60 -3.0 365,009 + Shewana3_0330 0.79 +0.2 365,017 - Shewana3_0330 0.81 -1.2 365,147 + +0.1 365,155 - +0.4 365,155 - +0.4 365,155 - -1.8 365,155 - -0.4 365,162 + +0.4
Or see this region's nucleotide sequence