Experiment: L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0326 and fabG are separated by 0 nucleotides fabG and Shewana3_0328 are separated by 125 nucleotides Shewana3_0328 and Shewana3_0329 are separated by 5 nucleotides
Shewana3_0326: Shewana3_0326 - 3-oxoacyl-(acyl carrier protein) synthase II (RefSeq), at 360,461 to 361,714
_0326
Shewana3_0327: fabG - 3-ketoacyl-(acyl-carrier-protein) reductase (RefSeq), at 361,715 to 362,440
fabG
Shewana3_0328: Shewana3_0328 - thioester dehydrase family protein (RefSeq), at 362,566 to 363,105
_0328
Shewana3_0329: Shewana3_0329 - 3-oxoacyl-(acyl carrier protein) synthase I (RefSeq), at 363,111 to 364,328
_0329
Position (kb) 361
362
363 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 360.763 kb on - strand, within Shewana3_0326 at 360.969 kb on - strand, within Shewana3_0326 at 361.180 kb on - strand, within Shewana3_0326 at 361.268 kb on + strand, within Shewana3_0326 at 361.286 kb on + strand, within Shewana3_0326 at 361.302 kb on - strand, within Shewana3_0326 at 361.362 kb on - strand, within Shewana3_0326 at 361.404 kb on - strand, within Shewana3_0326 at 361.612 kb on + strand at 361.612 kb on + strand at 361.612 kb on + strand at 361.612 kb on + strand at 361.620 kb on - strand at 361.620 kb on - strand at 361.620 kb on - strand at 361.620 kb on - strand at 361.654 kb on - strand at 361.691 kb on + strand at 361.699 kb on - strand at 361.838 kb on - strand, within fabG at 361.845 kb on + strand, within fabG at 361.845 kb on + strand, within fabG at 361.853 kb on - strand, within fabG at 361.930 kb on + strand, within fabG at 361.930 kb on + strand, within fabG at 361.930 kb on + strand, within fabG at 361.930 kb on + strand, within fabG at 361.938 kb on - strand, within fabG at 361.938 kb on - strand, within fabG at 361.938 kb on - strand, within fabG at 361.938 kb on - strand, within fabG at 361.957 kb on + strand, within fabG at 361.968 kb on + strand, within fabG at 361.993 kb on + strand, within fabG at 362.001 kb on - strand, within fabG at 362.001 kb on - strand, within fabG at 362.020 kb on - strand, within fabG at 362.191 kb on - strand, within fabG at 362.260 kb on + strand, within fabG at 362.302 kb on + strand, within fabG at 362.303 kb on - strand, within fabG at 362.310 kb on - strand, within fabG at 362.420 kb on + strand at 362.501 kb on + strand at 362.529 kb on + strand at 362.529 kb on + strand at 362.529 kb on + strand at 362.537 kb on - strand at 362.564 kb on + strand at 362.564 kb on + strand at 362.564 kb on + strand at 362.572 kb on - strand at 362.712 kb on - strand, within Shewana3_0328 at 362.764 kb on - strand, within Shewana3_0328 at 362.764 kb on - strand, within Shewana3_0328 at 362.773 kb on + strand, within Shewana3_0328 at 362.871 kb on - strand, within Shewana3_0328 at 363.171 kb on + strand at 363.298 kb on + strand, within Shewana3_0329
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N) remove 360,763 - Shewana3_0326 0.24 +0.0 360,969 - Shewana3_0326 0.41 +0.1 361,180 - Shewana3_0326 0.57 +0.4 361,268 + Shewana3_0326 0.64 -1.0 361,286 + Shewana3_0326 0.66 +0.6 361,302 - Shewana3_0326 0.67 -1.5 361,362 - Shewana3_0326 0.72 +0.5 361,404 - Shewana3_0326 0.75 +0.2 361,612 + -0.3 361,612 + -2.6 361,612 + -1.8 361,612 + -1.8 361,620 - +0.2 361,620 - -0.1 361,620 - +0.3 361,620 - -1.5 361,654 - -0.5 361,691 + +0.2 361,699 - -0.5 361,838 - fabG Shewana3_0327 0.17 +0.2 361,845 + fabG Shewana3_0327 0.18 +0.5 361,845 + fabG Shewana3_0327 0.18 +0.5 361,853 - fabG Shewana3_0327 0.19 +0.0 361,930 + fabG Shewana3_0327 0.30 -3.0 361,930 + fabG Shewana3_0327 0.30 -1.5 361,930 + fabG Shewana3_0327 0.30 -1.9 361,930 + fabG Shewana3_0327 0.30 -1.3 361,938 - fabG Shewana3_0327 0.31 +0.3 361,938 - fabG Shewana3_0327 0.31 +1.1 361,938 - fabG Shewana3_0327 0.31 -0.8 361,938 - fabG Shewana3_0327 0.31 +0.2 361,957 + fabG Shewana3_0327 0.33 -1.8 361,968 + fabG Shewana3_0327 0.35 +0.6 361,993 + fabG Shewana3_0327 0.38 +0.3 362,001 - fabG Shewana3_0327 0.39 +0.5 362,001 - fabG Shewana3_0327 0.39 -1.9 362,020 - fabG Shewana3_0327 0.42 +0.3 362,191 - fabG Shewana3_0327 0.66 -0.1 362,260 + fabG Shewana3_0327 0.75 +0.8 362,302 + fabG Shewana3_0327 0.81 +0.9 362,303 - fabG Shewana3_0327 0.81 -0.1 362,310 - fabG Shewana3_0327 0.82 +0.4 362,420 + +0.7 362,501 + +0.4 362,529 + +0.2 362,529 + +0.3 362,529 + +0.3 362,537 - -1.1 362,564 + +0.3 362,564 + -1.5 362,564 + +0.3 362,572 - +0.4 362,712 - Shewana3_0328 0.27 +0.1 362,764 - Shewana3_0328 0.37 -0.5 362,764 - Shewana3_0328 0.37 +0.2 362,773 + Shewana3_0328 0.38 -5.0 362,871 - Shewana3_0328 0.56 -0.7 363,171 + +0.5 363,298 + Shewana3_0329 0.15 +0.3
Or see this region's nucleotide sequence