Experiment: L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_4323 and Shewana3_4324 are separated by 590 nucleotides Shewana3_4324 and Shewana3_4325 overlap by 4 nucleotides Shewana3_4325 and Shewana3_4326 are separated by 2 nucleotides
Shewana3_4323: Shewana3_4323 - MerR family transcriptional regulator (RefSeq), at 213,486 to 213,926
_4323
Shewana3_4324: Shewana3_4324 - methionine sulfoxide reductase A (RefSeq), at 214,517 to 215,089
_4324
Shewana3_4325: Shewana3_4325 - methionine sulfoxide reductase A (RefSeq), at 215,086 to 215,586
_4325
Shewana3_4326: Shewana3_4326 - methionine sulfoxide reductase B (RefSeq), at 215,589 to 216,035
_4326
Position (kb) 214
215
216 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 213.531 kb on + strand, within Shewana3_4323 at 213.569 kb on + strand, within Shewana3_4323 at 213.706 kb on + strand, within Shewana3_4323 at 213.714 kb on - strand, within Shewana3_4323 at 213.716 kb on + strand, within Shewana3_4323 at 213.728 kb on + strand, within Shewana3_4323 at 213.736 kb on - strand, within Shewana3_4323 at 213.793 kb on - strand, within Shewana3_4323 at 213.841 kb on + strand, within Shewana3_4323 at 213.841 kb on + strand, within Shewana3_4323 at 213.849 kb on - strand, within Shewana3_4323 at 214.076 kb on - strand at 214.177 kb on + strand at 214.222 kb on + strand at 214.224 kb on + strand at 214.224 kb on + strand at 214.224 kb on + strand at 214.229 kb on + strand at 214.288 kb on - strand at 214.515 kb on + strand at 214.539 kb on - strand at 214.569 kb on - strand at 214.569 kb on - strand at 214.644 kb on + strand, within Shewana3_4324 at 214.644 kb on + strand, within Shewana3_4324 at 214.644 kb on + strand, within Shewana3_4324 at 214.652 kb on - strand, within Shewana3_4324 at 214.652 kb on - strand, within Shewana3_4324 at 214.652 kb on - strand, within Shewana3_4324 at 214.769 kb on - strand, within Shewana3_4324 at 214.787 kb on + strand, within Shewana3_4324 at 214.830 kb on - strand, within Shewana3_4324 at 214.840 kb on - strand, within Shewana3_4324 at 214.916 kb on + strand, within Shewana3_4324 at 215.096 kb on - strand at 215.174 kb on - strand, within Shewana3_4325 at 215.318 kb on + strand, within Shewana3_4325 at 215.473 kb on - strand, within Shewana3_4325 at 215.568 kb on - strand at 215.603 kb on - strand at 215.620 kb on + strand at 215.680 kb on - strand, within Shewana3_4326 at 215.702 kb on + strand, within Shewana3_4326 at 215.814 kb on + strand, within Shewana3_4326 at 215.859 kb on - strand, within Shewana3_4326 at 215.880 kb on + strand, within Shewana3_4326 at 215.964 kb on - strand, within Shewana3_4326 at 215.974 kb on + strand, within Shewana3_4326 at 215.974 kb on + strand, within Shewana3_4326 at 215.994 kb on - strand at 216.058 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N) remove 213,531 + Shewana3_4323 0.10 +0.3 213,569 + Shewana3_4323 0.19 +0.3 213,706 + Shewana3_4323 0.50 +0.0 213,714 - Shewana3_4323 0.52 +0.4 213,716 + Shewana3_4323 0.52 +0.1 213,728 + Shewana3_4323 0.55 -1.4 213,736 - Shewana3_4323 0.57 +0.7 213,793 - Shewana3_4323 0.70 +0.1 213,841 + Shewana3_4323 0.80 -1.2 213,841 + Shewana3_4323 0.80 +0.5 213,849 - Shewana3_4323 0.82 -0.4 214,076 - -1.3 214,177 + +1.0 214,222 + +0.1 214,224 + -2.4 214,224 + -2.8 214,224 + -3.1 214,229 + +0.1 214,288 - +0.3 214,515 + -1.3 214,539 - -0.3 214,569 - +0.9 214,569 - -0.2 214,644 + Shewana3_4324 0.22 -1.2 214,644 + Shewana3_4324 0.22 +0.7 214,644 + Shewana3_4324 0.22 +0.3 214,652 - Shewana3_4324 0.24 +0.9 214,652 - Shewana3_4324 0.24 +1.5 214,652 - Shewana3_4324 0.24 +0.6 214,769 - Shewana3_4324 0.44 +1.0 214,787 + Shewana3_4324 0.47 +0.2 214,830 - Shewana3_4324 0.55 +0.8 214,840 - Shewana3_4324 0.56 -1.5 214,916 + Shewana3_4324 0.70 +0.4 215,096 - -0.4 215,174 - Shewana3_4325 0.18 -2.0 215,318 + Shewana3_4325 0.46 +0.3 215,473 - Shewana3_4325 0.77 +0.8 215,568 - +0.8 215,603 - +0.6 215,620 + +0.2 215,680 - Shewana3_4326 0.20 -1.4 215,702 + Shewana3_4326 0.25 +0.7 215,814 + Shewana3_4326 0.50 +0.2 215,859 - Shewana3_4326 0.60 -1.6 215,880 + Shewana3_4326 0.65 +0.9 215,964 - Shewana3_4326 0.84 -1.2 215,974 + Shewana3_4326 0.86 +0.1 215,974 + Shewana3_4326 0.86 -1.0 215,994 - -0.3 216,058 + -1.4
Or see this region's nucleotide sequence