Experiment: L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_4307 and Shewana3_4308 are separated by 116 nucleotides Shewana3_4308 and Shewana3_4309 are separated by 355 nucleotides Shewana3_4309 and Shewana3_4310 are separated by 19 nucleotides
Shewana3_4307: Shewana3_4307 - transposase Tn3 family protein (RefSeq), at 195,317 to 198,304
_4307
Shewana3_4308: Shewana3_4308 - hypothetical protein (RefSeq), at 198,421 to 199,047
_4308
Shewana3_4309: Shewana3_4309 - MerR family transcriptional regulator (RefSeq), at 199,403 to 199,837
_4309
Shewana3_4310: Shewana3_4310 - diguanylate phosphodiesterase (RefSeq), at 199,857 to 200,657
_4310
Position (kb) 198
199
200 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 197.435 kb on + strand, within Shewana3_4307 at 197.438 kb on - strand, within Shewana3_4307 at 197.438 kb on - strand, within Shewana3_4307 at 197.505 kb on - strand, within Shewana3_4307 at 197.581 kb on - strand, within Shewana3_4307 at 197.604 kb on + strand, within Shewana3_4307 at 197.701 kb on + strand, within Shewana3_4307 at 197.901 kb on - strand, within Shewana3_4307 at 198.072 kb on + strand at 198.102 kb on - strand at 198.206 kb on + strand at 198.214 kb on - strand at 198.220 kb on + strand at 198.225 kb on + strand at 198.225 kb on + strand at 198.226 kb on + strand at 198.714 kb on - strand, within Shewana3_4308 at 198.967 kb on + strand, within Shewana3_4308 at 199.047 kb on + strand at 199.924 kb on - strand at 199.976 kb on + strand, within Shewana3_4310 at 199.976 kb on + strand, within Shewana3_4310 at 199.984 kb on - strand, within Shewana3_4310
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N) remove 197,435 + Shewana3_4307 0.71 -4.3 197,438 - Shewana3_4307 0.71 -1.6 197,438 - Shewana3_4307 0.71 -2.0 197,505 - Shewana3_4307 0.73 +1.1 197,581 - Shewana3_4307 0.76 +1.1 197,604 + Shewana3_4307 0.77 -1.6 197,701 + Shewana3_4307 0.80 +0.2 197,901 - Shewana3_4307 0.86 -0.0 198,072 + +0.7 198,102 - +0.1 198,206 + -1.5 198,214 - +0.6 198,220 + +0.3 198,225 + -1.6 198,225 + -0.7 198,226 + +0.2 198,714 - Shewana3_4308 0.47 +1.2 198,967 + Shewana3_4308 0.87 +0.6 199,047 + -1.5 199,924 - -0.4 199,976 + Shewana3_4310 0.15 -0.3 199,976 + Shewana3_4310 0.15 -2.0 199,984 - Shewana3_4310 0.16 +0.1
Or see this region's nucleotide sequence