Experiment: Thallium(I) acetate 10 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_R0019 and Shew_R0020 are separated by 45 nucleotides Shew_R0020 and Shew_R0021 are separated by 7 nucleotides Shew_R0021 and Shew_R0022 are separated by 8 nucleotides Shew_R0022 and Shew_0560 are separated by 700 nucleotides Shew_0560 and Shew_0561 are separated by 436 nucleotides
Shew_R0019: Shew_R0019 - tRNA-Gly (RefSeq), at 633,941 to 634,016
_R0019
Shew_R0020: Shew_R0020 - tRNA-Gly (RefSeq), at 634,062 to 634,137
_R0020
Shew_R0021: Shew_R0021 - tRNA-Gly (RefSeq), at 634,145 to 634,220
_R0021
Shew_R0022: Shew_R0022 - tRNA-Gly (RefSeq), at 634,229 to 634,304
_R0022
Shew_0560: Shew_0560 - hypothetical protein (RefSeq), at 635,005 to 635,460
_0560
Shew_0561: Shew_0561 - carbohydrate kinase, YjeF-related protein (RefSeq), at 635,897 to 637,375
_0561
Position (kb) 635
636 Strain fitness (log2 ratio) -2
-1
0
1
2 at 634.535 kb on + strand at 634.891 kb on + strand at 635.031 kb on + strand at 635.049 kb on - strand at 635.088 kb on - strand, within Shew_0560 at 635.090 kb on + strand, within Shew_0560 at 635.090 kb on + strand, within Shew_0560 at 635.098 kb on - strand, within Shew_0560 at 635.362 kb on - strand, within Shew_0560 at 635.454 kb on + strand at 635.462 kb on - strand at 635.772 kb on + strand at 635.854 kb on + strand at 635.899 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Thallium(I) acetate 10 mg/ml remove 634,535 + +2.0 634,891 + -0.3 635,031 + +0.4 635,049 - +0.3 635,088 - Shew_0560 0.18 -2.2 635,090 + Shew_0560 0.19 +0.2 635,090 + Shew_0560 0.19 +0.5 635,098 - Shew_0560 0.20 +1.6 635,362 - Shew_0560 0.78 +0.4 635,454 + +0.4 635,462 - +0.5 635,772 + +0.9 635,854 + +0.2 635,899 - +0.4
Or see this region's nucleotide sequence