Experiment: L-Phenylalanine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_66 and Pf6N2E2_67 are separated by 54 nucleotides Pf6N2E2_67 and Pf6N2E2_68 are separated by 153 nucleotides
Pf6N2E2_66 - 3-isopropylmalate dehydrogenase (EC 1.1.1.85), at 106,054 to 107,136
_66
Pf6N2E2_67 - Aspartate-semialdehyde dehydrogenase (EC 1.2.1.11), at 107,191 to 108,303
_67
Pf6N2E2_68 - Aspartate-semialdehyde dehydrogenase (EC 1.2.1.11), at 108,457 to 109,467
_68
Position (kb)
107
108
109 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 106.470 kb on + strand, within Pf6N2E2_66 at 106.470 kb on + strand, within Pf6N2E2_66 at 106.475 kb on - strand, within Pf6N2E2_66 at 106.475 kb on - strand, within Pf6N2E2_66 at 106.475 kb on - strand, within Pf6N2E2_66 at 106.529 kb on - strand, within Pf6N2E2_66 at 106.529 kb on - strand, within Pf6N2E2_66 at 106.543 kb on + strand, within Pf6N2E2_66 at 106.637 kb on + strand, within Pf6N2E2_66 at 106.717 kb on + strand, within Pf6N2E2_66 at 106.717 kb on + strand, within Pf6N2E2_66 at 106.717 kb on + strand, within Pf6N2E2_66 at 106.717 kb on + strand, within Pf6N2E2_66 at 106.717 kb on + strand, within Pf6N2E2_66 at 106.717 kb on + strand, within Pf6N2E2_66 at 106.718 kb on - strand, within Pf6N2E2_66 at 106.718 kb on - strand, within Pf6N2E2_66 at 106.718 kb on - strand, within Pf6N2E2_66 at 106.718 kb on - strand, within Pf6N2E2_66 at 106.718 kb on - strand, within Pf6N2E2_66 at 106.718 kb on - strand, within Pf6N2E2_66 at 106.718 kb on - strand, within Pf6N2E2_66 at 106.882 kb on + strand, within Pf6N2E2_66 at 106.882 kb on + strand, within Pf6N2E2_66 at 106.883 kb on - strand, within Pf6N2E2_66 at 106.883 kb on - strand, within Pf6N2E2_66 at 106.883 kb on - strand, within Pf6N2E2_66 at 106.883 kb on - strand, within Pf6N2E2_66 at 106.883 kb on - strand, within Pf6N2E2_66 at 106.975 kb on + strand, within Pf6N2E2_66 at 107.076 kb on + strand at 107.111 kb on + strand at 107.177 kb on + strand at 108.356 kb on - strand at 108.441 kb on + strand at 108.450 kb on + strand at 108.451 kb on - strand at 108.451 kb on - strand at 108.451 kb on - strand at 109.084 kb on - strand, within Pf6N2E2_68 at 109.084 kb on - strand, within Pf6N2E2_68 at 109.195 kb on - strand, within Pf6N2E2_68
Per-strain Table
Position Strand Gene LocusTag Fraction L-Phenylalanine (C) remove 106,470 + Pf6N2E2_66 0.38 -2.7 106,470 + Pf6N2E2_66 0.38 -2.6 106,475 - Pf6N2E2_66 0.39 -3.5 106,475 - Pf6N2E2_66 0.39 -4.3 106,475 - Pf6N2E2_66 0.39 -2.6 106,529 - Pf6N2E2_66 0.44 -3.5 106,529 - Pf6N2E2_66 0.44 -1.6 106,543 + Pf6N2E2_66 0.45 -3.8 106,637 + Pf6N2E2_66 0.54 -2.4 106,717 + Pf6N2E2_66 0.61 -2.9 106,717 + Pf6N2E2_66 0.61 -4.4 106,717 + Pf6N2E2_66 0.61 -1.9 106,717 + Pf6N2E2_66 0.61 +0.8 106,717 + Pf6N2E2_66 0.61 -2.7 106,717 + Pf6N2E2_66 0.61 -2.3 106,718 - Pf6N2E2_66 0.61 -4.0 106,718 - Pf6N2E2_66 0.61 -4.3 106,718 - Pf6N2E2_66 0.61 -2.6 106,718 - Pf6N2E2_66 0.61 -4.2 106,718 - Pf6N2E2_66 0.61 -2.6 106,718 - Pf6N2E2_66 0.61 -2.5 106,718 - Pf6N2E2_66 0.61 -2.7 106,882 + Pf6N2E2_66 0.76 -3.9 106,882 + Pf6N2E2_66 0.76 -3.9 106,883 - Pf6N2E2_66 0.77 -2.1 106,883 - Pf6N2E2_66 0.77 -2.3 106,883 - Pf6N2E2_66 0.77 -2.7 106,883 - Pf6N2E2_66 0.77 -1.5 106,883 - Pf6N2E2_66 0.77 -3.5 106,975 + Pf6N2E2_66 0.85 -4.5 107,076 + -3.8 107,111 + -2.3 107,177 + -1.5 108,356 - -0.9 108,441 + +0.7 108,450 + +0.7 108,451 - -0.9 108,451 - +0.2 108,451 - -0.2 109,084 - Pf6N2E2_68 0.62 -0.3 109,084 - Pf6N2E2_68 0.62 +0.1 109,195 - Pf6N2E2_68 0.73 +0.8
Or see this region's nucleotide sequence