Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_67

Experiment: L-Phenylalanine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_66 and Pf6N2E2_67 are separated by 54 nucleotidesPf6N2E2_67 and Pf6N2E2_68 are separated by 153 nucleotides Pf6N2E2_66 - 3-isopropylmalate dehydrogenase (EC 1.1.1.85), at 106,054 to 107,136 _66 Pf6N2E2_67 - Aspartate-semialdehyde dehydrogenase (EC 1.2.1.11), at 107,191 to 108,303 _67 Pf6N2E2_68 - Aspartate-semialdehyde dehydrogenase (EC 1.2.1.11), at 108,457 to 109,467 _68 Position (kb) 107 108 109Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 106.470 kb on + strand, within Pf6N2E2_66at 106.470 kb on + strand, within Pf6N2E2_66at 106.475 kb on - strand, within Pf6N2E2_66at 106.475 kb on - strand, within Pf6N2E2_66at 106.475 kb on - strand, within Pf6N2E2_66at 106.529 kb on - strand, within Pf6N2E2_66at 106.529 kb on - strand, within Pf6N2E2_66at 106.543 kb on + strand, within Pf6N2E2_66at 106.637 kb on + strand, within Pf6N2E2_66at 106.717 kb on + strand, within Pf6N2E2_66at 106.717 kb on + strand, within Pf6N2E2_66at 106.717 kb on + strand, within Pf6N2E2_66at 106.717 kb on + strand, within Pf6N2E2_66at 106.717 kb on + strand, within Pf6N2E2_66at 106.717 kb on + strand, within Pf6N2E2_66at 106.718 kb on - strand, within Pf6N2E2_66at 106.718 kb on - strand, within Pf6N2E2_66at 106.718 kb on - strand, within Pf6N2E2_66at 106.718 kb on - strand, within Pf6N2E2_66at 106.718 kb on - strand, within Pf6N2E2_66at 106.718 kb on - strand, within Pf6N2E2_66at 106.718 kb on - strand, within Pf6N2E2_66at 106.882 kb on + strand, within Pf6N2E2_66at 106.882 kb on + strand, within Pf6N2E2_66at 106.883 kb on - strand, within Pf6N2E2_66at 106.883 kb on - strand, within Pf6N2E2_66at 106.883 kb on - strand, within Pf6N2E2_66at 106.883 kb on - strand, within Pf6N2E2_66at 106.883 kb on - strand, within Pf6N2E2_66at 106.975 kb on + strand, within Pf6N2E2_66at 107.076 kb on + strandat 107.111 kb on + strandat 107.177 kb on + strandat 108.356 kb on - strandat 108.441 kb on + strandat 108.450 kb on + strandat 108.451 kb on - strandat 108.451 kb on - strandat 108.451 kb on - strandat 109.084 kb on - strand, within Pf6N2E2_68at 109.084 kb on - strand, within Pf6N2E2_68at 109.195 kb on - strand, within Pf6N2E2_68

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Phenylalanine (C)
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106,470 + Pf6N2E2_66 0.38 -2.7
106,470 + Pf6N2E2_66 0.38 -2.6
106,475 - Pf6N2E2_66 0.39 -3.5
106,475 - Pf6N2E2_66 0.39 -4.3
106,475 - Pf6N2E2_66 0.39 -2.6
106,529 - Pf6N2E2_66 0.44 -3.5
106,529 - Pf6N2E2_66 0.44 -1.6
106,543 + Pf6N2E2_66 0.45 -3.8
106,637 + Pf6N2E2_66 0.54 -2.4
106,717 + Pf6N2E2_66 0.61 -2.9
106,717 + Pf6N2E2_66 0.61 -4.4
106,717 + Pf6N2E2_66 0.61 -1.9
106,717 + Pf6N2E2_66 0.61 +0.8
106,717 + Pf6N2E2_66 0.61 -2.7
106,717 + Pf6N2E2_66 0.61 -2.3
106,718 - Pf6N2E2_66 0.61 -4.0
106,718 - Pf6N2E2_66 0.61 -4.3
106,718 - Pf6N2E2_66 0.61 -2.6
106,718 - Pf6N2E2_66 0.61 -4.2
106,718 - Pf6N2E2_66 0.61 -2.6
106,718 - Pf6N2E2_66 0.61 -2.5
106,718 - Pf6N2E2_66 0.61 -2.7
106,882 + Pf6N2E2_66 0.76 -3.9
106,882 + Pf6N2E2_66 0.76 -3.9
106,883 - Pf6N2E2_66 0.77 -2.1
106,883 - Pf6N2E2_66 0.77 -2.3
106,883 - Pf6N2E2_66 0.77 -2.7
106,883 - Pf6N2E2_66 0.77 -1.5
106,883 - Pf6N2E2_66 0.77 -3.5
106,975 + Pf6N2E2_66 0.85 -4.5
107,076 + -3.8
107,111 + -2.3
107,177 + -1.5
108,356 - -0.9
108,441 + +0.7
108,450 + +0.7
108,451 - -0.9
108,451 - +0.2
108,451 - -0.2
109,084 - Pf6N2E2_68 0.62 -0.3
109,084 - Pf6N2E2_68 0.62 +0.1
109,195 - Pf6N2E2_68 0.73 +0.8

Or see this region's nucleotide sequence