Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 Vitamin & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08100 and MMP_RS08105 are separated by 8 nucleotides MMP_RS08105 and MMP_RS09035 are separated by 186 nucleotides MMP_RS09035 and MMP_RS08110 are separated by 15 nucleotides
MMP_RS08100: MMP_RS08100 - 8-amino-7-oxononanoate synthase, at 1,524,069 to 1,525,181
_RS08100
MMP_RS08105: MMP_RS08105 - 6-carboxyhexanoate--CoA ligase, at 1,525,190 to 1,525,924
_RS08105
MMP_RS09035: MMP_RS09035 - hypothetical protein, at 1,526,111 to 1,526,266
_RS09035
MMP_RS08110: MMP_RS08110 - tRNA uridine(34) 5-carboxymethylaminomethyl modification radical SAM/GNAT enzyme Elp3, at 1,526,282 to 1,527,904
_RS08110
Position (kb)
1525
1526 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 1524.254 kb on - strand, within MMP_RS08100 at 1524.424 kb on - strand, within MMP_RS08100 at 1524.428 kb on - strand, within MMP_RS08100 at 1524.443 kb on - strand, within MMP_RS08100 at 1524.550 kb on + strand, within MMP_RS08100 at 1524.631 kb on - strand, within MMP_RS08100 at 1524.732 kb on + strand, within MMP_RS08100 at 1524.748 kb on + strand, within MMP_RS08100 at 1524.915 kb on - strand, within MMP_RS08100 at 1525.392 kb on + strand, within MMP_RS08105 at 1525.437 kb on + strand, within MMP_RS08105 at 1525.544 kb on - strand, within MMP_RS08105 at 1525.565 kb on + strand, within MMP_RS08105 at 1525.565 kb on + strand, within MMP_RS08105 at 1525.585 kb on - strand, within MMP_RS08105 at 1525.602 kb on + strand, within MMP_RS08105 at 1525.621 kb on - strand, within MMP_RS08105 at 1525.673 kb on - strand, within MMP_RS08105 at 1525.722 kb on + strand, within MMP_RS08105 at 1526.061 kb on + strand at 1526.064 kb on - strand at 1526.066 kb on - strand at 1526.067 kb on + strand at 1526.069 kb on + strand at 1526.069 kb on + strand at 1526.085 kb on + strand at 1526.119 kb on - strand at 1526.129 kb on + strand, within MMP_RS09035 at 1526.235 kb on - strand, within MMP_RS09035 at 1526.236 kb on + strand, within MMP_RS09035 at 1526.242 kb on - strand, within MMP_RS09035 at 1526.243 kb on + strand, within MMP_RS09035 at 1526.254 kb on + strand at 1526.269 kb on - strand at 1526.283 kb on - strand at 1526.337 kb on - strand at 1526.338 kb on + strand at 1526.341 kb on + strand at 1526.563 kb on - strand at 1526.571 kb on + strand at 1526.882 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 Vitamin & Casamino Acid FREE remove 1,524,254 - MMP_RS08100 0.17 -5.6 1,524,424 - MMP_RS08100 0.32 -2.5 1,524,428 - MMP_RS08100 0.32 -3.9 1,524,443 - MMP_RS08100 0.34 -2.3 1,524,550 + MMP_RS08100 0.43 -4.4 1,524,631 - MMP_RS08100 0.50 -4.8 1,524,732 + MMP_RS08100 0.60 -5.9 1,524,748 + MMP_RS08100 0.61 -3.9 1,524,915 - MMP_RS08100 0.76 -4.5 1,525,392 + MMP_RS08105 0.27 -2.1 1,525,437 + MMP_RS08105 0.34 -5.7 1,525,544 - MMP_RS08105 0.48 -5.2 1,525,565 + MMP_RS08105 0.51 -3.2 1,525,565 + MMP_RS08105 0.51 -5.3 1,525,585 - MMP_RS08105 0.54 -4.7 1,525,602 + MMP_RS08105 0.56 -4.4 1,525,621 - MMP_RS08105 0.59 -4.4 1,525,673 - MMP_RS08105 0.66 -4.0 1,525,722 + MMP_RS08105 0.72 -4.6 1,526,061 + -0.2 1,526,064 - +0.0 1,526,066 - +0.2 1,526,067 + +0.1 1,526,069 + -0.3 1,526,069 + +0.1 1,526,085 + -0.7 1,526,119 - +0.2 1,526,129 + MMP_RS09035 0.12 +1.0 1,526,235 - MMP_RS09035 0.79 +0.0 1,526,236 + MMP_RS09035 0.80 -0.4 1,526,242 - MMP_RS09035 0.84 -0.7 1,526,243 + MMP_RS09035 0.85 +0.3 1,526,254 + +0.3 1,526,269 - -0.2 1,526,283 - +0.1 1,526,337 - -2.3 1,526,338 + -1.0 1,526,341 + -2.9 1,526,563 - -4.5 1,526,571 + -3.8 1,526,882 + -3.8
Or see this region's nucleotide sequence