Experiment: R2A with benzoic 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS18740 and CA265_RS18745 are separated by 150 nucleotides CA265_RS18745 and CA265_RS18750 are separated by 158 nucleotides CA265_RS18750 and CA265_RS18755 are separated by 21 nucleotides
CA265_RS18740: CA265_RS18740 - pyruvate kinase, at 4,666,010 to 4,667,440
_RS18740
CA265_RS18745: CA265_RS18745 - hypothetical protein, at 4,667,591 to 4,668,010
_RS18745
CA265_RS18750: CA265_RS18750 - acyl carrier protein, at 4,668,169 to 4,668,405
_RS18750
CA265_RS18755: CA265_RS18755 - beta-ketoacyl-[acyl-carrier-protein] synthase II, at 4,668,427 to 4,669,680
_RS18755
Position (kb)
4667
4668
4669 Strain fitness (log2 ratio)
-2
-1
0
1 at 4667.480 kb on - strand at 4667.585 kb on + strand at 4667.786 kb on + strand, within CA265_RS18745 at 4667.790 kb on + strand, within CA265_RS18745 at 4667.934 kb on + strand, within CA265_RS18745 at 4668.055 kb on - strand at 4668.651 kb on - strand, within CA265_RS18755 at 4668.651 kb on - strand, within CA265_RS18755 at 4668.734 kb on + strand, within CA265_RS18755 at 4668.880 kb on + strand, within CA265_RS18755 at 4668.954 kb on + strand, within CA265_RS18755
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with benzoic 0.1 mg/ml remove 4,667,480 - +0.7 4,667,585 + -0.5 4,667,786 + CA265_RS18745 0.46 -0.2 4,667,790 + CA265_RS18745 0.47 -1.2 4,667,934 + CA265_RS18745 0.82 -1.8 4,668,055 - +0.9 4,668,651 - CA265_RS18755 0.18 -0.5 4,668,651 - CA265_RS18755 0.18 -0.2 4,668,734 + CA265_RS18755 0.24 +0.4 4,668,880 + CA265_RS18755 0.36 -2.3 4,668,954 + CA265_RS18755 0.42 -0.1
Or see this region's nucleotide sequence