Experiment: R2A with benzoic 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS14530 and CA265_RS14535 are separated by 138 nucleotides CA265_RS14535 and CA265_RS14540 are separated by 173 nucleotides
CA265_RS14530: CA265_RS14530 - nicotinate-nucleotide diphosphorylase (carboxylating), at 3,660,836 to 3,661,678
_RS14530
CA265_RS14535: CA265_RS14535 - DUF4783 domain-containing protein, at 3,661,817 to 3,662,215
_RS14535
CA265_RS14540: CA265_RS14540 - phosphoglycerate mutase (2,3-diphosphoglycerate-independent), at 3,662,389 to 3,663,912
_RS14540
Position (kb)
3661
3662
3663 Strain fitness (log2 ratio)
-2
-1
0
1 at 3660.838 kb on - strand at 3660.905 kb on + strand at 3660.922 kb on - strand, within CA265_RS14530 at 3660.934 kb on - strand, within CA265_RS14530 at 3661.031 kb on - strand at 3661.345 kb on + strand, within CA265_RS14530 at 3661.414 kb on + strand, within CA265_RS14530 at 3661.475 kb on + strand, within CA265_RS14530 at 3661.691 kb on - strand at 3661.718 kb on - strand at 3661.730 kb on - strand at 3661.753 kb on - strand at 3661.757 kb on + strand at 3661.757 kb on + strand at 3661.759 kb on + strand at 3661.913 kb on - strand, within CA265_RS14535 at 3662.170 kb on - strand, within CA265_RS14535 at 3662.171 kb on + strand, within CA265_RS14535 at 3662.171 kb on + strand, within CA265_RS14535 at 3662.186 kb on - strand at 3662.202 kb on - strand at 3662.239 kb on - strand at 3662.245 kb on - strand at 3662.307 kb on + strand at 3662.318 kb on + strand at 3662.318 kb on + strand at 3662.344 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with benzoic 0.1 mg/ml remove 3,660,838 - +0.7 3,660,905 + -1.6 3,660,922 - CA265_RS14530 0.10 -0.9 3,660,934 - CA265_RS14530 0.12 -1.2 3,661,031 - -0.2 3,661,345 + CA265_RS14530 0.60 -0.4 3,661,414 + CA265_RS14530 0.69 -0.8 3,661,475 + CA265_RS14530 0.76 +0.2 3,661,691 - -0.8 3,661,718 - +0.0 3,661,730 - -0.2 3,661,753 - +0.8 3,661,757 + -0.1 3,661,757 + -0.7 3,661,759 + +0.4 3,661,913 - CA265_RS14535 0.24 -0.3 3,662,170 - CA265_RS14535 0.88 +0.2 3,662,171 + CA265_RS14535 0.89 -1.5 3,662,171 + CA265_RS14535 0.89 -1.8 3,662,186 - +1.1 3,662,202 - -0.5 3,662,239 - +1.0 3,662,245 - -1.1 3,662,307 + -0.3 3,662,318 + +0.2 3,662,318 + +0.0 3,662,344 + +0.5
Or see this region's nucleotide sequence