Experiment: R2A with benzoic 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS13235 and CA265_RS13240 are separated by 209 nucleotides CA265_RS13240 and CA265_RS13245 are separated by 63 nucleotides CA265_RS13245 and CA265_RS13250 are separated by 2 nucleotides
CA265_RS13235: CA265_RS13235 - hypothetical protein, at 3,289,721 to 3,290,578
_RS13235
CA265_RS13240: CA265_RS13240 - preprotein translocase subunit YajC, at 3,290,788 to 3,291,108
_RS13240
CA265_RS13245: CA265_RS13245 - hypothetical protein, at 3,291,172 to 3,291,630
_RS13245
CA265_RS13250: CA265_RS13250 - transcription antitermination factor NusB, at 3,291,633 to 3,292,583
_RS13250
Position (kb)
3290
3291
3292 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3289.885 kb on + strand, within CA265_RS13235 at 3289.890 kb on - strand, within CA265_RS13235 at 3289.934 kb on - strand, within CA265_RS13235 at 3289.934 kb on - strand, within CA265_RS13235 at 3289.954 kb on + strand, within CA265_RS13235 at 3290.056 kb on + strand, within CA265_RS13235 at 3290.089 kb on + strand, within CA265_RS13235 at 3290.090 kb on - strand, within CA265_RS13235 at 3290.172 kb on - strand, within CA265_RS13235 at 3290.265 kb on - strand, within CA265_RS13235 at 3290.345 kb on - strand, within CA265_RS13235 at 3290.345 kb on - strand, within CA265_RS13235 at 3290.465 kb on + strand, within CA265_RS13235 at 3290.466 kb on - strand, within CA265_RS13235 at 3290.471 kb on + strand, within CA265_RS13235 at 3290.477 kb on + strand, within CA265_RS13235 at 3290.522 kb on - strand at 3290.558 kb on - strand at 3290.560 kb on - strand at 3290.624 kb on - strand at 3290.702 kb on - strand at 3290.733 kb on - strand at 3290.786 kb on + strand at 3290.787 kb on - strand at 3291.025 kb on + strand, within CA265_RS13240 at 3291.055 kb on - strand, within CA265_RS13240 at 3291.070 kb on - strand, within CA265_RS13240 at 3291.092 kb on - strand at 3291.113 kb on + strand at 3291.114 kb on - strand at 3291.117 kb on + strand at 3291.196 kb on + strand at 3291.252 kb on + strand, within CA265_RS13245 at 3291.252 kb on + strand, within CA265_RS13245 at 3291.252 kb on + strand, within CA265_RS13245 at 3291.253 kb on - strand, within CA265_RS13245 at 3291.271 kb on - strand, within CA265_RS13245 at 3291.290 kb on - strand, within CA265_RS13245 at 3291.294 kb on + strand, within CA265_RS13245 at 3291.294 kb on + strand, within CA265_RS13245 at 3291.349 kb on - strand, within CA265_RS13245 at 3291.394 kb on - strand, within CA265_RS13245 at 3291.442 kb on + strand, within CA265_RS13245 at 3291.508 kb on - strand, within CA265_RS13245 at 3291.522 kb on + strand, within CA265_RS13245 at 3291.568 kb on - strand, within CA265_RS13245 at 3291.634 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with benzoic 0.1 mg/ml remove 3,289,885 + CA265_RS13235 0.19 -1.5 3,289,890 - CA265_RS13235 0.20 -0.0 3,289,934 - CA265_RS13235 0.25 +0.3 3,289,934 - CA265_RS13235 0.25 +0.5 3,289,954 + CA265_RS13235 0.27 -1.1 3,290,056 + CA265_RS13235 0.39 -2.0 3,290,089 + CA265_RS13235 0.43 -0.2 3,290,090 - CA265_RS13235 0.43 -0.5 3,290,172 - CA265_RS13235 0.53 -0.8 3,290,265 - CA265_RS13235 0.63 -1.4 3,290,345 - CA265_RS13235 0.73 -0.2 3,290,345 - CA265_RS13235 0.73 -0.3 3,290,465 + CA265_RS13235 0.87 -0.2 3,290,466 - CA265_RS13235 0.87 +0.5 3,290,471 + CA265_RS13235 0.87 +0.8 3,290,477 + CA265_RS13235 0.88 -1.0 3,290,522 - -1.5 3,290,558 - +0.3 3,290,560 - -0.5 3,290,624 - -0.6 3,290,702 - -0.1 3,290,733 - -0.1 3,290,786 + +2.3 3,290,787 - -0.5 3,291,025 + CA265_RS13240 0.74 +0.6 3,291,055 - CA265_RS13240 0.83 -1.4 3,291,070 - CA265_RS13240 0.88 -1.2 3,291,092 - +1.5 3,291,113 + -0.4 3,291,114 - +0.6 3,291,117 + +0.3 3,291,196 + +0.0 3,291,252 + CA265_RS13245 0.17 +0.0 3,291,252 + CA265_RS13245 0.17 +0.5 3,291,252 + CA265_RS13245 0.17 -0.7 3,291,253 - CA265_RS13245 0.18 +0.7 3,291,271 - CA265_RS13245 0.22 +0.0 3,291,290 - CA265_RS13245 0.26 +0.3 3,291,294 + CA265_RS13245 0.27 -0.2 3,291,294 + CA265_RS13245 0.27 -1.3 3,291,349 - CA265_RS13245 0.39 -0.2 3,291,394 - CA265_RS13245 0.48 +0.4 3,291,442 + CA265_RS13245 0.59 -0.7 3,291,508 - CA265_RS13245 0.73 +0.1 3,291,522 + CA265_RS13245 0.76 +0.1 3,291,568 - CA265_RS13245 0.86 +0.7 3,291,634 + -0.2
Or see this region's nucleotide sequence