Experiment: R2A with benzoic 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS12715 and CA265_RS12720 are separated by 357 nucleotides CA265_RS12720 and CA265_RS12725 overlap by 10 nucleotides
CA265_RS12715: CA265_RS12715 - hypothetical protein, at 3,169,901 to 3,171,040
_RS12715
CA265_RS12720: CA265_RS12720 - hypothetical protein, at 3,171,398 to 3,172,204
_RS12720
CA265_RS12725: CA265_RS12725 - 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD, at 3,172,195 to 3,172,731
_RS12725
Position (kb)
3171
3172
3173 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3170.419 kb on + strand, within CA265_RS12715 at 3170.420 kb on - strand, within CA265_RS12715 at 3170.484 kb on - strand, within CA265_RS12715 at 3170.486 kb on + strand, within CA265_RS12715 at 3170.501 kb on - strand, within CA265_RS12715 at 3170.515 kb on + strand, within CA265_RS12715 at 3170.522 kb on - strand, within CA265_RS12715 at 3170.561 kb on + strand, within CA265_RS12715 at 3170.595 kb on + strand, within CA265_RS12715 at 3170.600 kb on - strand, within CA265_RS12715 at 3170.687 kb on - strand, within CA265_RS12715 at 3170.774 kb on + strand, within CA265_RS12715 at 3170.774 kb on + strand, within CA265_RS12715 at 3170.774 kb on + strand, within CA265_RS12715 at 3170.775 kb on - strand, within CA265_RS12715 at 3170.940 kb on - strand at 3170.993 kb on - strand at 3170.993 kb on - strand at 3171.021 kb on + strand at 3171.021 kb on + strand at 3171.061 kb on - strand at 3171.181 kb on + strand at 3171.270 kb on + strand at 3171.271 kb on - strand at 3171.286 kb on + strand at 3171.362 kb on + strand at 3171.369 kb on - strand at 3171.410 kb on - strand at 3171.410 kb on - strand at 3171.410 kb on - strand at 3171.497 kb on + strand, within CA265_RS12720 at 3171.497 kb on + strand, within CA265_RS12720 at 3171.530 kb on - strand, within CA265_RS12720 at 3171.565 kb on - strand, within CA265_RS12720 at 3171.613 kb on - strand, within CA265_RS12720 at 3171.618 kb on - strand, within CA265_RS12720 at 3171.643 kb on + strand, within CA265_RS12720 at 3171.710 kb on + strand, within CA265_RS12720 at 3171.711 kb on - strand, within CA265_RS12720 at 3171.734 kb on + strand, within CA265_RS12720 at 3171.745 kb on + strand, within CA265_RS12720 at 3171.746 kb on - strand, within CA265_RS12720 at 3171.764 kb on - strand, within CA265_RS12720 at 3171.780 kb on + strand, within CA265_RS12720 at 3171.901 kb on - strand, within CA265_RS12720 at 3172.017 kb on + strand, within CA265_RS12720 at 3172.042 kb on - strand, within CA265_RS12720 at 3172.096 kb on + strand, within CA265_RS12720 at 3172.167 kb on - strand at 3172.195 kb on - strand at 3172.202 kb on + strand at 3172.203 kb on - strand at 3172.266 kb on + strand, within CA265_RS12725 at 3172.330 kb on - strand, within CA265_RS12725 at 3172.398 kb on + strand, within CA265_RS12725 at 3172.420 kb on + strand, within CA265_RS12725 at 3172.561 kb on - strand, within CA265_RS12725 at 3172.561 kb on - strand, within CA265_RS12725 at 3172.561 kb on - strand, within CA265_RS12725 at 3172.593 kb on + strand, within CA265_RS12725 at 3172.611 kb on + strand, within CA265_RS12725 at 3172.611 kb on + strand, within CA265_RS12725 at 3172.612 kb on - strand, within CA265_RS12725 at 3172.650 kb on + strand, within CA265_RS12725 at 3172.725 kb on + strand at 3172.726 kb on - strand at 3172.765 kb on + strand at 3172.766 kb on - strand at 3172.864 kb on + strand at 3172.865 kb on - strand at 3172.865 kb on - strand at 3172.905 kb on - strand at 3172.905 kb on - strand at 3172.919 kb on + strand at 3172.919 kb on + strand at 3172.923 kb on + strand at 3172.923 kb on + strand at 3172.924 kb on - strand at 3172.932 kb on + strand at 3172.933 kb on - strand at 3172.934 kb on + strand at 3173.009 kb on - strand at 3173.012 kb on + strand at 3173.015 kb on + strand at 3173.072 kb on + strand at 3173.096 kb on - strand at 3173.096 kb on - strand at 3173.111 kb on - strand at 3173.114 kb on + strand at 3173.114 kb on + strand at 3173.115 kb on - strand at 3173.123 kb on + strand at 3173.123 kb on + strand at 3173.124 kb on - strand at 3173.126 kb on - strand at 3173.156 kb on + strand at 3173.157 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with benzoic 0.1 mg/ml remove 3,170,419 + CA265_RS12715 0.45 +0.3 3,170,420 - CA265_RS12715 0.46 -0.3 3,170,484 - CA265_RS12715 0.51 -0.3 3,170,486 + CA265_RS12715 0.51 -2.5 3,170,501 - CA265_RS12715 0.53 +0.1 3,170,515 + CA265_RS12715 0.54 -1.1 3,170,522 - CA265_RS12715 0.54 -0.4 3,170,561 + CA265_RS12715 0.58 -0.3 3,170,595 + CA265_RS12715 0.61 +1.0 3,170,600 - CA265_RS12715 0.61 -2.0 3,170,687 - CA265_RS12715 0.69 +0.5 3,170,774 + CA265_RS12715 0.77 -0.8 3,170,774 + CA265_RS12715 0.77 -0.5 3,170,774 + CA265_RS12715 0.77 +0.3 3,170,775 - CA265_RS12715 0.77 +0.2 3,170,940 - -0.6 3,170,993 - -0.4 3,170,993 - -0.7 3,171,021 + -1.7 3,171,021 + +0.4 3,171,061 - -0.5 3,171,181 + -0.2 3,171,270 + -0.2 3,171,271 - -0.2 3,171,286 + -0.1 3,171,362 + +0.2 3,171,369 - -0.9 3,171,410 - -0.3 3,171,410 - -1.5 3,171,410 - -1.6 3,171,497 + CA265_RS12720 0.12 -1.2 3,171,497 + CA265_RS12720 0.12 -0.7 3,171,530 - CA265_RS12720 0.16 -1.6 3,171,565 - CA265_RS12720 0.21 +1.9 3,171,613 - CA265_RS12720 0.27 -0.7 3,171,618 - CA265_RS12720 0.27 +0.0 3,171,643 + CA265_RS12720 0.30 +0.2 3,171,710 + CA265_RS12720 0.39 +0.4 3,171,711 - CA265_RS12720 0.39 +0.7 3,171,734 + CA265_RS12720 0.42 -0.3 3,171,745 + CA265_RS12720 0.43 -1.7 3,171,746 - CA265_RS12720 0.43 -0.6 3,171,764 - CA265_RS12720 0.45 -1.1 3,171,780 + CA265_RS12720 0.47 -0.7 3,171,901 - CA265_RS12720 0.62 -0.5 3,172,017 + CA265_RS12720 0.77 -1.3 3,172,042 - CA265_RS12720 0.80 -1.2 3,172,096 + CA265_RS12720 0.86 +0.5 3,172,167 - +0.1 3,172,195 - +0.1 3,172,202 + -0.4 3,172,203 - -0.4 3,172,266 + CA265_RS12725 0.13 -0.9 3,172,330 - CA265_RS12725 0.25 -0.7 3,172,398 + CA265_RS12725 0.38 -0.5 3,172,420 + CA265_RS12725 0.42 +0.1 3,172,561 - CA265_RS12725 0.68 -0.6 3,172,561 - CA265_RS12725 0.68 -0.4 3,172,561 - CA265_RS12725 0.68 -0.1 3,172,593 + CA265_RS12725 0.74 -0.3 3,172,611 + CA265_RS12725 0.77 +0.4 3,172,611 + CA265_RS12725 0.77 +0.2 3,172,612 - CA265_RS12725 0.78 -1.3 3,172,650 + CA265_RS12725 0.85 -0.5 3,172,725 + -0.4 3,172,726 - -0.5 3,172,765 + +0.1 3,172,766 - -0.4 3,172,864 + +0.8 3,172,865 - +0.1 3,172,865 - -0.3 3,172,905 - -0.3 3,172,905 - +1.0 3,172,919 + -0.2 3,172,919 + +0.2 3,172,923 + +0.1 3,172,923 + -0.2 3,172,924 - -0.2 3,172,932 + -0.4 3,172,933 - -0.6 3,172,934 + +0.0 3,173,009 - -0.2 3,173,012 + -0.0 3,173,015 + -0.6 3,173,072 + +1.1 3,173,096 - +0.1 3,173,096 - -0.2 3,173,111 - +0.2 3,173,114 + -0.3 3,173,114 + -1.4 3,173,115 - -0.3 3,173,123 + +0.1 3,173,123 + -0.6 3,173,124 - -0.4 3,173,126 - -0.3 3,173,156 + -0.1 3,173,157 - +0.1
Or see this region's nucleotide sequence