Experiment: R2A with benzoic 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS10140 and CA265_RS10145 are separated by 15 nucleotides CA265_RS10145 and CA265_RS10150 are separated by 10 nucleotides CA265_RS10150 and CA265_RS10155 are separated by 12 nucleotides
CA265_RS10140: CA265_RS10140 - hypothetical protein, at 2,494,952 to 2,495,269
_RS10140
CA265_RS10145: CA265_RS10145 - hypothetical protein, at 2,495,285 to 2,495,674
_RS10145
CA265_RS10150: CA265_RS10150 - cysteine synthase B, at 2,495,685 to 2,496,557
_RS10150
CA265_RS10155: CA265_RS10155 - serine acetyltransferase, at 2,496,570 to 2,497,382
_RS10155
Position (kb)
2495
2496
2497 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2494.729 kb on - strand at 2494.730 kb on + strand at 2494.730 kb on + strand at 2494.730 kb on + strand at 2494.731 kb on - strand at 2494.731 kb on - strand at 2494.797 kb on + strand at 2494.801 kb on - strand at 2495.031 kb on - strand, within CA265_RS10140 at 2495.040 kb on + strand, within CA265_RS10140 at 2495.040 kb on + strand, within CA265_RS10140 at 2495.091 kb on + strand, within CA265_RS10140 at 2495.104 kb on + strand, within CA265_RS10140 at 2495.105 kb on - strand, within CA265_RS10140 at 2495.227 kb on - strand, within CA265_RS10140 at 2495.227 kb on - strand, within CA265_RS10140 at 2495.245 kb on + strand at 2495.257 kb on + strand at 2495.257 kb on + strand at 2495.314 kb on - strand at 2495.317 kb on + strand at 2495.350 kb on - strand, within CA265_RS10145 at 2495.375 kb on + strand, within CA265_RS10145 at 2495.375 kb on + strand, within CA265_RS10145 at 2495.375 kb on + strand, within CA265_RS10145 at 2495.376 kb on - strand, within CA265_RS10145 at 2495.409 kb on + strand, within CA265_RS10145 at 2495.409 kb on + strand, within CA265_RS10145 at 2495.413 kb on + strand, within CA265_RS10145 at 2495.487 kb on + strand, within CA265_RS10145 at 2495.488 kb on - strand, within CA265_RS10145 at 2495.492 kb on - strand, within CA265_RS10145 at 2495.492 kb on - strand, within CA265_RS10145 at 2495.499 kb on + strand, within CA265_RS10145 at 2495.499 kb on + strand, within CA265_RS10145 at 2495.500 kb on - strand, within CA265_RS10145 at 2495.500 kb on - strand, within CA265_RS10145 at 2495.511 kb on + strand, within CA265_RS10145 at 2495.570 kb on + strand, within CA265_RS10145 at 2495.571 kb on - strand, within CA265_RS10145 at 2495.594 kb on + strand, within CA265_RS10145 at 2495.595 kb on - strand, within CA265_RS10145 at 2495.672 kb on + strand at 2495.706 kb on + strand at 2495.874 kb on - strand, within CA265_RS10150 at 2495.921 kb on - strand, within CA265_RS10150 at 2496.127 kb on + strand, within CA265_RS10150 at 2496.128 kb on - strand, within CA265_RS10150 at 2496.194 kb on - strand, within CA265_RS10150 at 2496.261 kb on - strand, within CA265_RS10150 at 2496.303 kb on + strand, within CA265_RS10150 at 2496.309 kb on - strand at 2496.416 kb on + strand, within CA265_RS10150 at 2496.547 kb on - strand at 2496.558 kb on - strand at 2496.562 kb on + strand at 2496.567 kb on + strand at 2496.568 kb on - strand at 2496.571 kb on + strand at 2496.638 kb on + strand at 2496.647 kb on + strand at 2496.672 kb on - strand, within CA265_RS10155 at 2496.702 kb on + strand, within CA265_RS10155 at 2496.703 kb on - strand, within CA265_RS10155 at 2496.705 kb on + strand, within CA265_RS10155 at 2496.883 kb on + strand, within CA265_RS10155 at 2496.893 kb on + strand, within CA265_RS10155 at 2496.918 kb on - strand, within CA265_RS10155 at 2496.948 kb on + strand, within CA265_RS10155 at 2496.966 kb on + strand, within CA265_RS10155 at 2496.967 kb on - strand, within CA265_RS10155 at 2496.986 kb on + strand, within CA265_RS10155 at 2497.098 kb on - strand, within CA265_RS10155 at 2497.119 kb on + strand at 2497.120 kb on - strand, within CA265_RS10155 at 2497.191 kb on - strand, within CA265_RS10155 at 2497.196 kb on - strand, within CA265_RS10155 at 2497.196 kb on - strand, within CA265_RS10155 at 2497.221 kb on - strand, within CA265_RS10155 at 2497.223 kb on - strand, within CA265_RS10155 at 2497.231 kb on - strand, within CA265_RS10155 at 2497.252 kb on - strand, within CA265_RS10155 at 2497.278 kb on + strand, within CA265_RS10155 at 2497.294 kb on + strand, within CA265_RS10155 at 2497.329 kb on - strand at 2497.382 kb on + strand at 2497.393 kb on + strand at 2497.419 kb on + strand at 2497.420 kb on - strand at 2497.423 kb on + strand at 2497.423 kb on + strand at 2497.492 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with benzoic 0.1 mg/ml remove 2,494,729 - +0.2 2,494,730 + -2.0 2,494,730 + -0.9 2,494,730 + -1.6 2,494,731 - +1.7 2,494,731 - +0.0 2,494,797 + -0.4 2,494,801 - -0.6 2,495,031 - CA265_RS10140 0.25 -0.2 2,495,040 + CA265_RS10140 0.28 +0.8 2,495,040 + CA265_RS10140 0.28 -0.4 2,495,091 + CA265_RS10140 0.44 -0.4 2,495,104 + CA265_RS10140 0.48 -1.7 2,495,105 - CA265_RS10140 0.48 -0.5 2,495,227 - CA265_RS10140 0.86 +1.0 2,495,227 - CA265_RS10140 0.86 +0.1 2,495,245 + -0.6 2,495,257 + -0.2 2,495,257 + -0.6 2,495,314 - -0.2 2,495,317 + -0.9 2,495,350 - CA265_RS10145 0.17 -0.2 2,495,375 + CA265_RS10145 0.23 -2.0 2,495,375 + CA265_RS10145 0.23 -0.6 2,495,375 + CA265_RS10145 0.23 -0.9 2,495,376 - CA265_RS10145 0.23 +0.9 2,495,409 + CA265_RS10145 0.32 +0.5 2,495,409 + CA265_RS10145 0.32 -0.4 2,495,413 + CA265_RS10145 0.33 +0.3 2,495,487 + CA265_RS10145 0.52 -1.9 2,495,488 - CA265_RS10145 0.52 -0.5 2,495,492 - CA265_RS10145 0.53 +0.3 2,495,492 - CA265_RS10145 0.53 -0.8 2,495,499 + CA265_RS10145 0.55 -0.3 2,495,499 + CA265_RS10145 0.55 -0.9 2,495,500 - CA265_RS10145 0.55 -0.2 2,495,500 - CA265_RS10145 0.55 -0.6 2,495,511 + CA265_RS10145 0.58 +0.1 2,495,570 + CA265_RS10145 0.73 -0.2 2,495,571 - CA265_RS10145 0.73 -0.8 2,495,594 + CA265_RS10145 0.79 -0.1 2,495,595 - CA265_RS10145 0.79 +0.2 2,495,672 + -0.6 2,495,706 + -1.4 2,495,874 - CA265_RS10150 0.22 -1.1 2,495,921 - CA265_RS10150 0.27 -2.5 2,496,127 + CA265_RS10150 0.51 -0.7 2,496,128 - CA265_RS10150 0.51 -1.9 2,496,194 - CA265_RS10150 0.58 -2.8 2,496,261 - CA265_RS10150 0.66 -1.0 2,496,303 + CA265_RS10150 0.71 -2.0 2,496,309 - -1.6 2,496,416 + CA265_RS10150 0.84 -0.5 2,496,547 - -0.9 2,496,558 - -2.0 2,496,562 + -1.7 2,496,567 + +1.2 2,496,568 - -0.2 2,496,571 + -0.5 2,496,638 + -2.7 2,496,647 + -1.0 2,496,672 - CA265_RS10155 0.13 -1.7 2,496,702 + CA265_RS10155 0.16 -1.1 2,496,703 - CA265_RS10155 0.16 -2.5 2,496,705 + CA265_RS10155 0.17 -0.4 2,496,883 + CA265_RS10155 0.38 -0.6 2,496,893 + CA265_RS10155 0.40 -2.4 2,496,918 - CA265_RS10155 0.43 -0.5 2,496,948 + CA265_RS10155 0.46 -1.3 2,496,966 + CA265_RS10155 0.49 -2.5 2,496,967 - CA265_RS10155 0.49 -0.9 2,496,986 + CA265_RS10155 0.51 -1.9 2,497,098 - CA265_RS10155 0.65 -2.5 2,497,119 + +1.2 2,497,120 - CA265_RS10155 0.68 -0.6 2,497,191 - CA265_RS10155 0.76 -0.9 2,497,196 - CA265_RS10155 0.77 -1.6 2,497,196 - CA265_RS10155 0.77 -1.3 2,497,221 - CA265_RS10155 0.80 -1.3 2,497,223 - CA265_RS10155 0.80 -0.9 2,497,231 - CA265_RS10155 0.81 -0.8 2,497,252 - CA265_RS10155 0.84 -1.1 2,497,278 + CA265_RS10155 0.87 -0.4 2,497,294 + CA265_RS10155 0.89 -1.4 2,497,329 - -1.9 2,497,382 + -0.1 2,497,393 + -2.3 2,497,419 + -1.3 2,497,420 - -0.4 2,497,423 + -1.3 2,497,423 + -1.3 2,497,492 - +0.5
Or see this region's nucleotide sequence