Experiment: R2A with benzoic 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS09310 and CA265_RS09315 are separated by 38 nucleotides CA265_RS09315 and CA265_RS09320 are separated by 48 nucleotides CA265_RS09320 and CA265_RS09325 overlap by 11 nucleotides
CA265_RS09310: CA265_RS09310 - pseudouridylate synthase, at 2,315,718 to 2,316,401
_RS09310
CA265_RS09315: CA265_RS09315 - hypothetical protein, at 2,316,440 to 2,317,174
_RS09315
CA265_RS09320: CA265_RS09320 - hypothetical protein, at 2,317,223 to 2,317,417
_RS09320
CA265_RS09325: CA265_RS09325 - MATE family efflux transporter, at 2,317,407 to 2,318,768
_RS09325
Position (kb)
2317
2318 Strain fitness (log2 ratio)
-2
-1
0
1 at 2316.353 kb on - strand at 2316.432 kb on + strand at 2316.433 kb on - strand at 2316.439 kb on + strand at 2316.439 kb on + strand at 2316.440 kb on - strand at 2316.440 kb on - strand at 2316.453 kb on + strand at 2316.506 kb on + strand at 2316.506 kb on + strand at 2316.507 kb on - strand at 2316.507 kb on - strand at 2316.509 kb on - strand at 2316.526 kb on + strand, within CA265_RS09315 at 2316.527 kb on - strand, within CA265_RS09315 at 2316.537 kb on + strand, within CA265_RS09315 at 2316.542 kb on - strand, within CA265_RS09315 at 2316.634 kb on - strand, within CA265_RS09315 at 2316.788 kb on - strand, within CA265_RS09315 at 2316.815 kb on - strand, within CA265_RS09315 at 2316.864 kb on + strand, within CA265_RS09315 at 2316.865 kb on - strand, within CA265_RS09315 at 2316.929 kb on - strand, within CA265_RS09315 at 2316.929 kb on - strand, within CA265_RS09315 at 2316.933 kb on - strand, within CA265_RS09315 at 2316.958 kb on + strand, within CA265_RS09315 at 2316.962 kb on - strand, within CA265_RS09315 at 2317.043 kb on - strand, within CA265_RS09315 at 2317.075 kb on + strand, within CA265_RS09315 at 2317.090 kb on + strand, within CA265_RS09315 at 2317.090 kb on + strand, within CA265_RS09315 at 2317.168 kb on + strand at 2317.169 kb on - strand at 2317.172 kb on + strand at 2317.173 kb on - strand at 2317.193 kb on + strand at 2317.194 kb on - strand at 2317.210 kb on - strand at 2317.213 kb on + strand at 2317.214 kb on - strand at 2317.325 kb on - strand, within CA265_RS09320 at 2317.325 kb on - strand, within CA265_RS09320 at 2317.396 kb on + strand, within CA265_RS09320 at 2317.581 kb on - strand, within CA265_RS09325 at 2317.584 kb on - strand, within CA265_RS09325 at 2317.596 kb on - strand, within CA265_RS09325 at 2317.599 kb on + strand, within CA265_RS09325 at 2317.600 kb on - strand, within CA265_RS09325 at 2317.626 kb on + strand, within CA265_RS09325 at 2317.725 kb on - strand, within CA265_RS09325 at 2317.766 kb on + strand, within CA265_RS09325 at 2317.776 kb on - strand, within CA265_RS09325 at 2317.776 kb on - strand, within CA265_RS09325 at 2317.788 kb on + strand, within CA265_RS09325 at 2317.815 kb on - strand, within CA265_RS09325 at 2317.865 kb on + strand, within CA265_RS09325 at 2317.878 kb on + strand, within CA265_RS09325 at 2317.879 kb on - strand, within CA265_RS09325 at 2317.896 kb on - strand, within CA265_RS09325 at 2317.897 kb on + strand, within CA265_RS09325 at 2317.898 kb on - strand, within CA265_RS09325 at 2317.898 kb on - strand, within CA265_RS09325 at 2317.917 kb on - strand, within CA265_RS09325 at 2318.006 kb on - strand, within CA265_RS09325 at 2318.053 kb on - strand, within CA265_RS09325 at 2318.115 kb on + strand, within CA265_RS09325 at 2318.119 kb on + strand, within CA265_RS09325 at 2318.162 kb on + strand, within CA265_RS09325 at 2318.166 kb on + strand, within CA265_RS09325 at 2318.230 kb on - strand, within CA265_RS09325 at 2318.237 kb on + strand, within CA265_RS09325 at 2318.237 kb on + strand, within CA265_RS09325 at 2318.240 kb on + strand, within CA265_RS09325 at 2318.240 kb on + strand, within CA265_RS09325 at 2318.379 kb on + strand, within CA265_RS09325 at 2318.382 kb on + strand, within CA265_RS09325
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with benzoic 0.1 mg/ml remove 2,316,353 - +0.4 2,316,432 + -1.1 2,316,433 - -0.8 2,316,439 + -0.1 2,316,439 + +0.7 2,316,440 - +0.0 2,316,440 - +0.3 2,316,453 + -0.1 2,316,506 + -0.1 2,316,506 + +0.2 2,316,507 - +0.5 2,316,507 - +0.1 2,316,509 - -0.1 2,316,526 + CA265_RS09315 0.12 -0.8 2,316,527 - CA265_RS09315 0.12 -0.1 2,316,537 + CA265_RS09315 0.13 -0.2 2,316,542 - CA265_RS09315 0.14 +0.4 2,316,634 - CA265_RS09315 0.26 -0.4 2,316,788 - CA265_RS09315 0.47 -0.0 2,316,815 - CA265_RS09315 0.51 +0.2 2,316,864 + CA265_RS09315 0.58 -0.5 2,316,865 - CA265_RS09315 0.58 -0.5 2,316,929 - CA265_RS09315 0.67 +0.9 2,316,929 - CA265_RS09315 0.67 -0.3 2,316,933 - CA265_RS09315 0.67 -0.0 2,316,958 + CA265_RS09315 0.70 +0.0 2,316,962 - CA265_RS09315 0.71 -0.6 2,317,043 - CA265_RS09315 0.82 +0.1 2,317,075 + CA265_RS09315 0.86 +0.8 2,317,090 + CA265_RS09315 0.88 -0.3 2,317,090 + CA265_RS09315 0.88 -0.0 2,317,168 + +0.2 2,317,169 - -0.1 2,317,172 + +0.2 2,317,173 - +0.1 2,317,193 + -0.2 2,317,194 - -0.2 2,317,210 - -0.2 2,317,213 + -0.5 2,317,214 - +0.3 2,317,325 - CA265_RS09320 0.52 +0.2 2,317,325 - CA265_RS09320 0.52 +0.3 2,317,396 + CA265_RS09320 0.89 +1.2 2,317,581 - CA265_RS09325 0.13 -0.9 2,317,584 - CA265_RS09325 0.13 +0.7 2,317,596 - CA265_RS09325 0.14 +0.4 2,317,599 + CA265_RS09325 0.14 +0.2 2,317,600 - CA265_RS09325 0.14 -2.0 2,317,626 + CA265_RS09325 0.16 -0.1 2,317,725 - CA265_RS09325 0.23 +0.2 2,317,766 + CA265_RS09325 0.26 -0.4 2,317,776 - CA265_RS09325 0.27 +0.0 2,317,776 - CA265_RS09325 0.27 +0.1 2,317,788 + CA265_RS09325 0.28 -0.4 2,317,815 - CA265_RS09325 0.30 +0.0 2,317,865 + CA265_RS09325 0.34 +0.2 2,317,878 + CA265_RS09325 0.35 -0.2 2,317,879 - CA265_RS09325 0.35 -0.5 2,317,896 - CA265_RS09325 0.36 +1.6 2,317,897 + CA265_RS09325 0.36 -0.7 2,317,898 - CA265_RS09325 0.36 -0.1 2,317,898 - CA265_RS09325 0.36 -1.7 2,317,917 - CA265_RS09325 0.37 +0.0 2,318,006 - CA265_RS09325 0.44 -0.3 2,318,053 - CA265_RS09325 0.47 -0.1 2,318,115 + CA265_RS09325 0.52 +0.2 2,318,119 + CA265_RS09325 0.52 +0.6 2,318,162 + CA265_RS09325 0.55 -0.1 2,318,166 + CA265_RS09325 0.56 +0.2 2,318,230 - CA265_RS09325 0.60 +0.6 2,318,237 + CA265_RS09325 0.61 +0.3 2,318,237 + CA265_RS09325 0.61 +0.0 2,318,240 + CA265_RS09325 0.61 -0.1 2,318,240 + CA265_RS09325 0.61 +0.2 2,318,379 + CA265_RS09325 0.71 +0.4 2,318,382 + CA265_RS09325 0.72 -0.5
Or see this region's nucleotide sequence