Experiment: R2A with benzoic 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS00325 and CA265_RS00330 are separated by 93 nucleotides CA265_RS00330 and CA265_RS00335 are separated by 89 nucleotides CA265_RS00335 and CA265_RS00340 are separated by 52 nucleotides CA265_RS00340 and CA265_RS00345 are separated by 192 nucleotides
CA265_RS00325: CA265_RS00325 - hypothetical protein, at 74,985 to 75,440
_RS00325
CA265_RS00330: CA265_RS00330 - helix-turn-helix transcriptional regulator, at 75,534 to 76,295
_RS00330
CA265_RS00335: CA265_RS00335 - hypothetical protein, at 76,385 to 76,576
_RS00335
CA265_RS00340: CA265_RS00340 - hypothetical protein, at 76,629 to 77,345
_RS00340
CA265_RS00345: CA265_RS00345 - alpha-galactosidase, at 77,538 to 79,664
_RS00345
Position (kb)
76
77 Strain fitness (log2 ratio)
-2
-1
0
1 at 75.387 kb on + strand, within CA265_RS00325 at 75.430 kb on + strand at 75.431 kb on - strand at 75.449 kb on + strand at 75.531 kb on + strand at 75.532 kb on - strand at 75.535 kb on + strand at 75.535 kb on + strand at 75.535 kb on + strand at 75.581 kb on + strand at 75.582 kb on - strand at 75.582 kb on - strand at 75.620 kb on - strand, within CA265_RS00330 at 75.803 kb on + strand, within CA265_RS00330 at 75.804 kb on - strand, within CA265_RS00330 at 75.804 kb on - strand, within CA265_RS00330 at 75.839 kb on + strand, within CA265_RS00330 at 75.840 kb on - strand, within CA265_RS00330 at 75.840 kb on - strand, within CA265_RS00330 at 75.914 kb on + strand, within CA265_RS00330 at 75.948 kb on - strand, within CA265_RS00330 at 75.953 kb on + strand, within CA265_RS00330 at 76.024 kb on - strand, within CA265_RS00330 at 76.080 kb on + strand, within CA265_RS00330 at 76.080 kb on + strand, within CA265_RS00330 at 76.081 kb on - strand, within CA265_RS00330 at 76.104 kb on + strand, within CA265_RS00330 at 76.162 kb on + strand, within CA265_RS00330 at 76.162 kb on + strand, within CA265_RS00330 at 76.165 kb on - strand, within CA265_RS00330 at 76.413 kb on + strand, within CA265_RS00335 at 76.420 kb on - strand, within CA265_RS00335 at 76.535 kb on - strand, within CA265_RS00335 at 76.587 kb on - strand at 76.605 kb on + strand at 76.700 kb on + strand at 76.719 kb on + strand, within CA265_RS00340 at 76.721 kb on + strand, within CA265_RS00340 at 76.721 kb on + strand, within CA265_RS00340 at 76.722 kb on - strand, within CA265_RS00340 at 76.742 kb on + strand, within CA265_RS00340 at 76.804 kb on + strand, within CA265_RS00340 at 76.858 kb on + strand, within CA265_RS00340 at 76.863 kb on - strand, within CA265_RS00340 at 76.924 kb on - strand, within CA265_RS00340 at 76.956 kb on - strand, within CA265_RS00340 at 76.964 kb on - strand, within CA265_RS00340 at 77.021 kb on + strand, within CA265_RS00340 at 77.022 kb on - strand, within CA265_RS00340 at 77.062 kb on - strand, within CA265_RS00340 at 77.078 kb on - strand, within CA265_RS00340 at 77.201 kb on + strand, within CA265_RS00340 at 77.202 kb on - strand, within CA265_RS00340 at 77.240 kb on - strand, within CA265_RS00340 at 77.242 kb on + strand, within CA265_RS00340 at 77.243 kb on - strand, within CA265_RS00340 at 77.251 kb on + strand, within CA265_RS00340 at 77.251 kb on + strand, within CA265_RS00340 at 77.252 kb on - strand, within CA265_RS00340 at 77.288 kb on - strand at 77.343 kb on + strand at 77.365 kb on - strand at 77.401 kb on + strand at 77.401 kb on + strand at 77.463 kb on - strand at 77.463 kb on - strand at 77.463 kb on - strand at 77.469 kb on + strand at 77.469 kb on + strand at 77.480 kb on + strand at 77.569 kb on + strand at 77.570 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with benzoic 0.1 mg/ml remove 75,387 + CA265_RS00325 0.88 -0.9 75,430 + +0.7 75,431 - -0.5 75,449 + -0.4 75,531 + -1.4 75,532 - -0.5 75,535 + +0.1 75,535 + -0.7 75,535 + +0.8 75,581 + +0.2 75,582 - +0.1 75,582 - -0.2 75,620 - CA265_RS00330 0.11 -0.2 75,803 + CA265_RS00330 0.35 -1.5 75,804 - CA265_RS00330 0.35 -0.0 75,804 - CA265_RS00330 0.35 -0.4 75,839 + CA265_RS00330 0.40 +0.7 75,840 - CA265_RS00330 0.40 +0.4 75,840 - CA265_RS00330 0.40 +0.4 75,914 + CA265_RS00330 0.50 -0.6 75,948 - CA265_RS00330 0.54 -0.3 75,953 + CA265_RS00330 0.55 -0.5 76,024 - CA265_RS00330 0.64 -0.6 76,080 + CA265_RS00330 0.72 +0.5 76,080 + CA265_RS00330 0.72 +0.0 76,081 - CA265_RS00330 0.72 -0.8 76,104 + CA265_RS00330 0.75 -0.7 76,162 + CA265_RS00330 0.82 -0.5 76,162 + CA265_RS00330 0.82 +0.6 76,165 - CA265_RS00330 0.83 -0.5 76,413 + CA265_RS00335 0.15 -0.3 76,420 - CA265_RS00335 0.18 -0.4 76,535 - CA265_RS00335 0.78 -1.6 76,587 - +0.1 76,605 + +0.6 76,700 + -0.1 76,719 + CA265_RS00340 0.13 -0.1 76,721 + CA265_RS00340 0.13 +0.6 76,721 + CA265_RS00340 0.13 -0.9 76,722 - CA265_RS00340 0.13 +0.8 76,742 + CA265_RS00340 0.16 -0.3 76,804 + CA265_RS00340 0.24 -0.4 76,858 + CA265_RS00340 0.32 +0.6 76,863 - CA265_RS00340 0.33 -0.6 76,924 - CA265_RS00340 0.41 -1.8 76,956 - CA265_RS00340 0.46 +0.8 76,964 - CA265_RS00340 0.47 -0.2 77,021 + CA265_RS00340 0.55 -0.5 77,022 - CA265_RS00340 0.55 -1.1 77,062 - CA265_RS00340 0.60 -0.3 77,078 - CA265_RS00340 0.63 -0.2 77,201 + CA265_RS00340 0.80 -2.3 77,202 - CA265_RS00340 0.80 -0.5 77,240 - CA265_RS00340 0.85 -1.0 77,242 + CA265_RS00340 0.85 +0.1 77,243 - CA265_RS00340 0.86 -0.6 77,251 + CA265_RS00340 0.87 +0.9 77,251 + CA265_RS00340 0.87 -1.3 77,252 - CA265_RS00340 0.87 -0.6 77,288 - +0.4 77,343 + +0.1 77,365 - +0.0 77,401 + -0.0 77,401 + -0.2 77,463 - -0.7 77,463 - +0.4 77,463 - +1.6 77,469 + -0.3 77,469 + +0.3 77,480 + -0.0 77,569 + -0.1 77,570 - -0.1
Or see this region's nucleotide sequence