Experiment: D-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS02110 and AZOBR_RS02115 are separated by 267 nucleotides AZOBR_RS02115 and AZOBR_RS02120 are separated by 58 nucleotides AZOBR_RS02120 and AZOBR_RS02125 are separated by 213 nucleotides
AZOBR_RS02110: AZOBR_RS02110 - protein-PII uridylyltransferase, at 432,723 to 435,524
_RS02110
AZOBR_RS02115: AZOBR_RS02115 - hypothetical protein, at 435,792 to 436,103
_RS02115
AZOBR_RS02120: AZOBR_RS02120 - hypothetical protein, at 436,162 to 436,635
_RS02120
AZOBR_RS02125: AZOBR_RS02125 - DNA mismatch repair protein MutS, at 436,849 to 439,551
_RS02125
Position (kb) 435
436
437 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 435.046 kb on + strand, within AZOBR_RS02110 at 435.046 kb on + strand, within AZOBR_RS02110 at 435.047 kb on - strand, within AZOBR_RS02110 at 435.055 kb on + strand, within AZOBR_RS02110 at 435.055 kb on + strand, within AZOBR_RS02110 at 435.056 kb on - strand at 435.056 kb on - strand, within AZOBR_RS02110 at 435.586 kb on + strand at 435.603 kb on + strand at 435.603 kb on + strand at 435.779 kb on + strand at 435.779 kb on + strand at 435.780 kb on - strand at 436.163 kb on + strand at 436.446 kb on - strand, within AZOBR_RS02120 at 436.763 kb on - strand at 436.763 kb on - strand at 436.799 kb on + strand at 436.800 kb on - strand at 436.960 kb on + strand at 436.978 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (N) remove 435,046 + AZOBR_RS02110 0.83 -0.6 435,046 + AZOBR_RS02110 0.83 -3.2 435,047 - AZOBR_RS02110 0.83 -2.0 435,055 + AZOBR_RS02110 0.83 -0.6 435,055 + AZOBR_RS02110 0.83 +1.0 435,056 - -0.6 435,056 - AZOBR_RS02110 0.83 -1.4 435,586 + -1.0 435,603 + -1.0 435,603 + -1.8 435,779 + -0.1 435,779 + -1.3 435,780 - -0.2 436,163 + -0.1 436,446 - AZOBR_RS02120 0.60 +0.3 436,763 - -0.2 436,763 - -0.6 436,799 + +0.2 436,800 - -0.2 436,960 + -1.4 436,978 + -1.1
Or see this region's nucleotide sequence