Experiment: D-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS01300 and AZOBR_RS01305 are separated by 148 nucleotides AZOBR_RS01305 and AZOBR_RS01310 are separated by 93 nucleotides AZOBR_RS01310 and AZOBR_RS01315 are separated by 299 nucleotides
AZOBR_RS01300: AZOBR_RS01300 - peptide ABC transporter substrate-binding protein, at 269,287 to 271,095
_RS01300
AZOBR_RS01305: AZOBR_RS01305 - inorganic pyrophosphatase, at 271,244 to 271,801
_RS01305
AZOBR_RS01310: AZOBR_RS01310 - lactate dehydrogenase, at 271,895 to 272,836
_RS01310
AZOBR_RS01315: AZOBR_RS01315 - GCN5 family N-acetyltransferase, at 273,136 to 275,820
_RS01315
Position (kb) 271
272
273 Strain fitness (log2 ratio) -1
0
1 at 270.983 kb on + strand at 271.142 kb on + strand at 272.184 kb on + strand, within AZOBR_RS01310 at 272.960 kb on + strand at 272.981 kb on + strand at 273.041 kb on - strand at 273.310 kb on + strand at 273.310 kb on + strand at 273.311 kb on - strand at 273.311 kb on - strand at 273.718 kb on + strand, within AZOBR_RS01315 at 273.719 kb on - strand, within AZOBR_RS01315 at 273.719 kb on - strand at 273.719 kb on - strand, within AZOBR_RS01315 at 273.757 kb on + strand, within AZOBR_RS01315 at 273.758 kb on - strand, within AZOBR_RS01315
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (N) remove 270,983 + +0.6 271,142 + +0.7 272,184 + AZOBR_RS01310 0.31 -0.0 272,960 + +0.6 272,981 + -0.0 273,041 - +0.0 273,310 + +1.6 273,310 + +1.0 273,311 - -0.2 273,311 - +0.7 273,718 + AZOBR_RS01315 0.22 -0.8 273,719 - AZOBR_RS01315 0.22 +0.3 273,719 - -0.6 273,719 - AZOBR_RS01315 0.22 -0.3 273,757 + AZOBR_RS01315 0.23 +0.7 273,758 - AZOBR_RS01315 0.23 -1.2
Or see this region's nucleotide sequence