Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_4009 and Shewana3_4010 are separated by 25 nucleotides Shewana3_4010 and Shewana3_4011 are separated by 61 nucleotides Shewana3_4011 and Shewana3_4012 are separated by 66 nucleotides
Shewana3_4009: Shewana3_4009 - competence protein ComF (RefSeq), at 4,812,987 to 4,813,775
_4009
Shewana3_4010: Shewana3_4010 - bioH protein (RefSeq), at 4,813,801 to 4,814,595
_4010
Shewana3_4011: Shewana3_4011 - hypothetical protein (RefSeq), at 4,814,657 to 4,815,211
_4011
Shewana3_4012: Shewana3_4012 - sulfatase (RefSeq), at 4,815,278 to 4,817,242
_4012
Position (kb)
4814
4815
4816 Strain fitness (log2 ratio)
-2
-1
0
1 at 4813.666 kb on + strand, within Shewana3_4009 at 4813.780 kb on - strand at 4814.598 kb on - strand at 4815.144 kb on + strand, within Shewana3_4011 at 4815.154 kb on + strand, within Shewana3_4011 at 4815.183 kb on - strand at 4815.310 kb on + strand at 4815.335 kb on + strand at 4815.513 kb on + strand, within Shewana3_4012 at 4815.544 kb on - strand, within Shewana3_4012 at 4815.544 kb on - strand, within Shewana3_4012 at 4815.546 kb on + strand, within Shewana3_4012 at 4815.701 kb on - strand, within Shewana3_4012 at 4815.823 kb on + strand, within Shewana3_4012 at 4815.823 kb on + strand, within Shewana3_4012 at 4815.841 kb on + strand, within Shewana3_4012 at 4815.849 kb on - strand, within Shewana3_4012 at 4815.849 kb on - strand, within Shewana3_4012 at 4815.956 kb on + strand, within Shewana3_4012
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 4,813,666 + Shewana3_4009 0.86 -0.8 4,813,780 - +0.7 4,814,598 - -1.7 4,815,144 + Shewana3_4011 0.88 -1.7 4,815,154 + Shewana3_4011 0.90 +0.3 4,815,183 - +0.4 4,815,310 + -1.5 4,815,335 + +0.6 4,815,513 + Shewana3_4012 0.12 -1.2 4,815,544 - Shewana3_4012 0.14 -1.6 4,815,544 - Shewana3_4012 0.14 -1.7 4,815,546 + Shewana3_4012 0.14 +0.5 4,815,701 - Shewana3_4012 0.22 -2.3 4,815,823 + Shewana3_4012 0.28 -0.9 4,815,823 + Shewana3_4012 0.28 -1.8 4,815,841 + Shewana3_4012 0.29 +0.1 4,815,849 - Shewana3_4012 0.29 -0.1 4,815,849 - Shewana3_4012 0.29 +0.3 4,815,956 + Shewana3_4012 0.35 -1.7
Or see this region's nucleotide sequence