Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2362 and Shewana3_2363 are separated by 24 nucleotides Shewana3_2363 and Shewana3_R0100 are separated by 282 nucleotides Shewana3_R0100 and Shewana3_R0101 are separated by 56 nucleotides Shewana3_R0101 and Shewana3_2364 are separated by 165 nucleotides
Shewana3_2362: Shewana3_2362 - NnrS family protein (RefSeq), at 2,839,141 to 2,840,352
_2362
Shewana3_2363: Shewana3_2363 - cytochrome c552 (RefSeq), at 2,840,377 to 2,841,810
_2363
Shewana3_R0100: Shewana3_R0100 - tRNA-Asp (RefSeq), at 2,842,093 to 2,842,169
_R0100
Shewana3_R0101: Shewana3_R0101 - tRNA-Asp (RefSeq), at 2,842,226 to 2,842,302
_R0101
Shewana3_2364: Shewana3_2364 - hypothetical protein (RefSeq), at 2,842,468 to 2,843,652
_2364
Position (kb)
2840
2841
2842 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2839.564 kb on - strand, within Shewana3_2362 at 2839.854 kb on - strand, within Shewana3_2362 at 2839.856 kb on + strand, within Shewana3_2362 at 2839.909 kb on - strand, within Shewana3_2362 at 2839.991 kb on + strand, within Shewana3_2362 at 2840.059 kb on + strand, within Shewana3_2362 at 2840.202 kb on + strand, within Shewana3_2362 at 2840.304 kb on + strand at 2840.314 kb on + strand at 2840.579 kb on - strand, within Shewana3_2363 at 2840.663 kb on - strand, within Shewana3_2363 at 2840.668 kb on + strand, within Shewana3_2363 at 2840.712 kb on + strand, within Shewana3_2363 at 2840.712 kb on + strand, within Shewana3_2363 at 2840.714 kb on + strand, within Shewana3_2363 at 2840.714 kb on + strand, within Shewana3_2363 at 2840.739 kb on + strand, within Shewana3_2363 at 2840.806 kb on - strand, within Shewana3_2363 at 2840.891 kb on - strand, within Shewana3_2363 at 2840.920 kb on - strand, within Shewana3_2363 at 2841.444 kb on - strand, within Shewana3_2363 at 2841.602 kb on + strand, within Shewana3_2363 at 2841.620 kb on - strand, within Shewana3_2363 at 2841.856 kb on + strand at 2842.574 kb on - strand at 2842.755 kb on + strand, within Shewana3_2364
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 2,839,564 - Shewana3_2362 0.35 -3.1 2,839,854 - Shewana3_2362 0.59 +0.5 2,839,856 + Shewana3_2362 0.59 +0.3 2,839,909 - Shewana3_2362 0.63 -0.1 2,839,991 + Shewana3_2362 0.70 +0.1 2,840,059 + Shewana3_2362 0.76 +1.5 2,840,202 + Shewana3_2362 0.88 -1.2 2,840,304 + -0.2 2,840,314 + +0.4 2,840,579 - Shewana3_2363 0.14 -0.1 2,840,663 - Shewana3_2363 0.20 -0.5 2,840,668 + Shewana3_2363 0.20 +0.6 2,840,712 + Shewana3_2363 0.23 +0.8 2,840,712 + Shewana3_2363 0.23 +0.7 2,840,714 + Shewana3_2363 0.24 +0.9 2,840,714 + Shewana3_2363 0.24 +0.3 2,840,739 + Shewana3_2363 0.25 -1.9 2,840,806 - Shewana3_2363 0.30 +0.8 2,840,891 - Shewana3_2363 0.36 +0.4 2,840,920 - Shewana3_2363 0.38 -1.0 2,841,444 - Shewana3_2363 0.74 +0.8 2,841,602 + Shewana3_2363 0.85 -0.2 2,841,620 - Shewana3_2363 0.87 -0.0 2,841,856 + +0.8 2,842,574 - -1.2 2,842,755 + Shewana3_2364 0.24 -1.0
Or see this region's nucleotide sequence