Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1740 and Shewana3_1741 are separated by 77 nucleotides Shewana3_1741 and Shewana3_1742 are separated by 194 nucleotides Shewana3_1742 and Shewana3_1743 are separated by 279 nucleotides Shewana3_1743 and Shewana3_1744 are separated by 96 nucleotides
Shewana3_1740: Shewana3_1740 - peptidase S8 and S53, subtilisin, kexin, sedolisin (RefSeq), at 2,051,257 to 2,055,042
_1740
Shewana3_1741: Shewana3_1741 - hypothetical protein (RefSeq), at 2,055,120 to 2,055,467
_1741
Shewana3_1742: Shewana3_1742 - leucine dehydrogenase (RefSeq), at 2,055,662 to 2,056,696
_1742
Shewana3_1743: Shewana3_1743 - hypothetical protein (RefSeq), at 2,056,976 to 2,057,434
_1743
Shewana3_1744: Shewana3_1744 - cupin 4 family protein (RefSeq), at 2,057,531 to 2,058,670
_1744
Position (kb)
2055
2056
2057 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2055.002 kb on - strand at 2055.007 kb on - strand at 2055.146 kb on + strand at 2055.291 kb on - strand, within Shewana3_1741 at 2055.448 kb on - strand at 2055.659 kb on + strand at 2055.948 kb on + strand, within Shewana3_1742 at 2055.974 kb on - strand, within Shewana3_1742 at 2056.111 kb on + strand, within Shewana3_1742 at 2056.144 kb on + strand, within Shewana3_1742 at 2056.160 kb on + strand, within Shewana3_1742 at 2056.189 kb on + strand, within Shewana3_1742 at 2056.197 kb on - strand, within Shewana3_1742 at 2056.197 kb on - strand, within Shewana3_1742 at 2056.203 kb on + strand, within Shewana3_1742 at 2056.707 kb on + strand at 2057.016 kb on + strand at 2057.511 kb on + strand at 2057.528 kb on + strand at 2057.536 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 2,055,002 - +0.5 2,055,007 - -0.0 2,055,146 + -1.1 2,055,291 - Shewana3_1741 0.49 -1.6 2,055,448 - -2.7 2,055,659 + -1.0 2,055,948 + Shewana3_1742 0.28 -4.3 2,055,974 - Shewana3_1742 0.30 -2.7 2,056,111 + Shewana3_1742 0.43 -2.2 2,056,144 + Shewana3_1742 0.47 -3.8 2,056,160 + Shewana3_1742 0.48 -2.2 2,056,189 + Shewana3_1742 0.51 -2.6 2,056,197 - Shewana3_1742 0.52 -2.5 2,056,197 - Shewana3_1742 0.52 -3.6 2,056,203 + Shewana3_1742 0.52 -1.6 2,056,707 + -2.7 2,057,016 + +0.1 2,057,511 + -0.3 2,057,528 + -2.4 2,057,536 - +0.3
Or see this region's nucleotide sequence