Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1687 and Shewana3_1688 are separated by 114 nucleotides Shewana3_1688 and Shewana3_1689 are separated by 1 nucleotides Shewana3_1689 and Shewana3_1690 are separated by 688 nucleotides
Shewana3_1687: Shewana3_1687 - short-chain dehydrogenase/reductase SDR (RefSeq), at 1,982,069 to 1,983,022
_1687
Shewana3_1688: Shewana3_1688 - acyl-CoA thioesterase II (RefSeq), at 1,983,137 to 1,984,003
_1688
Shewana3_1689: Shewana3_1689 - hypothetical protein (RefSeq), at 1,984,005 to 1,984,409
_1689
Shewana3_1690: Shewana3_1690 - protease domain-containing protein (RefSeq), at 1,985,098 to 1,989,000
_1690
Position (kb)
1984
1985 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1983.080 kb on + strand at 1983.100 kb on + strand at 1983.131 kb on + strand at 1983.286 kb on - strand, within Shewana3_1688 at 1983.448 kb on + strand, within Shewana3_1688 at 1983.670 kb on - strand, within Shewana3_1688 at 1983.951 kb on - strand at 1983.982 kb on - strand at 1984.137 kb on + strand, within Shewana3_1689 at 1984.145 kb on - strand, within Shewana3_1689 at 1984.197 kb on + strand, within Shewana3_1689 at 1984.337 kb on + strand, within Shewana3_1689 at 1984.382 kb on + strand at 1984.672 kb on - strand at 1985.124 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 1,983,080 + -0.3 1,983,100 + +1.1 1,983,131 + -1.9 1,983,286 - Shewana3_1688 0.17 +0.7 1,983,448 + Shewana3_1688 0.36 +0.8 1,983,670 - Shewana3_1688 0.61 +1.3 1,983,951 - +2.9 1,983,982 - +2.6 1,984,137 + Shewana3_1689 0.33 +3.7 1,984,145 - Shewana3_1689 0.35 -0.0 1,984,197 + Shewana3_1689 0.47 +2.8 1,984,337 + Shewana3_1689 0.82 +3.2 1,984,382 + +1.3 1,984,672 - +0.3 1,985,124 + -2.6
Or see this region's nucleotide sequence