Strain Fitness in Shewanella sp. ANA-3 around Shewana3_1553

Experiment: L-Isoleucine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntShewana3_1552 and Shewana3_1553 are separated by 87 nucleotidesShewana3_1553 and pflA are separated by 15 nucleotidespflA and Shewana3_1555 are separated by 63 nucleotides Shewana3_1552: Shewana3_1552 - acetate kinase (RefSeq), at 1,808,697 to 1,809,896 _1552 Shewana3_1553: Shewana3_1553 - hypothetical protein (RefSeq), at 1,809,984 to 1,810,481 _1553 Shewana3_1554: pflA - pyruvate formate lyase-activating enzyme 1 (RefSeq), at 1,810,497 to 1,811,237 pflA Shewana3_1555: Shewana3_1555 - formate acetyltransferase (RefSeq), at 1,811,301 to 1,813,583 _1555 Position (kb) 1809 1810 1811Strain fitness (log2 ratio) -3 -2 -1 0 1at 1809.022 kb on + strand, within Shewana3_1552at 1809.193 kb on - strand, within Shewana3_1552at 1809.365 kb on - strand, within Shewana3_1552at 1809.451 kb on + strand, within Shewana3_1552at 1810.002 kb on - strandat 1810.009 kb on - strandat 1810.011 kb on + strandat 1810.213 kb on - strand, within Shewana3_1553at 1810.266 kb on + strand, within Shewana3_1553at 1810.475 kb on + strandat 1810.568 kb on - strandat 1810.797 kb on - strand, within pflAat 1810.865 kb on - strand, within pflAat 1810.965 kb on - strand, within pflAat 1811.141 kb on + strand, within pflAat 1811.156 kb on + strand, within pflAat 1811.318 kb on + strandat 1811.374 kb on + strandat 1811.382 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (N)
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1,809,022 + Shewana3_1552 0.27 -2.0
1,809,193 - Shewana3_1552 0.41 -0.7
1,809,365 - Shewana3_1552 0.56 -1.5
1,809,451 + Shewana3_1552 0.63 -1.9
1,810,002 - +1.1
1,810,009 - +0.4
1,810,011 + -0.7
1,810,213 - Shewana3_1553 0.46 -1.9
1,810,266 + Shewana3_1553 0.57 -2.9
1,810,475 + -1.5
1,810,568 - +0.5
1,810,797 - pflA Shewana3_1554 0.40 +0.5
1,810,865 - pflA Shewana3_1554 0.50 -1.2
1,810,965 - pflA Shewana3_1554 0.63 -1.0
1,811,141 + pflA Shewana3_1554 0.87 +0.7
1,811,156 + pflA Shewana3_1554 0.89 -0.1
1,811,318 + -0.2
1,811,374 + -1.3
1,811,382 - +0.7

Or see this region's nucleotide sequence