Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1112 and Shewana3_1113 are separated by 134 nucleotides Shewana3_1113 and mazG are separated by 272 nucleotides mazG and pyrG are separated by 116 nucleotides
Shewana3_1112: Shewana3_1112 - hypothetical protein (RefSeq), at 1,309,586 to 1,312,291
_1112
Shewana3_1113: Shewana3_1113 - hypothetical protein (RefSeq), at 1,312,426 to 1,312,629
_1113
Shewana3_1114: mazG - nucleoside triphosphate pyrophosphohydrolase (RefSeq), at 1,312,902 to 1,313,780
mazG
Shewana3_1115: pyrG - CTP synthetase (RefSeq), at 1,313,897 to 1,315,537
pyrG
Position (kb)
1312
1313
1314 Strain fitness (log2 ratio)
-2
-1
0
1 at 1311.902 kb on + strand, within Shewana3_1112 at 1312.205 kb on - strand at 1312.207 kb on + strand at 1312.274 kb on - strand at 1312.347 kb on + strand at 1312.387 kb on - strand at 1312.449 kb on - strand, within Shewana3_1113 at 1312.612 kb on - strand at 1312.660 kb on + strand at 1312.668 kb on - strand at 1312.729 kb on + strand at 1312.729 kb on + strand at 1312.813 kb on - strand at 1312.830 kb on - strand at 1312.864 kb on + strand at 1313.176 kb on - strand, within mazG at 1313.265 kb on - strand, within mazG at 1313.457 kb on + strand, within mazG at 1313.457 kb on + strand, within mazG at 1313.611 kb on + strand, within mazG at 1313.725 kb on + strand at 1313.733 kb on - strand at 1313.733 kb on - strand at 1313.755 kb on + strand at 1313.755 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 1,311,902 + Shewana3_1112 0.86 +0.0 1,312,205 - -2.3 1,312,207 + +0.3 1,312,274 - +0.5 1,312,347 + +0.3 1,312,387 - +0.2 1,312,449 - Shewana3_1113 0.11 +0.7 1,312,612 - -1.3 1,312,660 + -1.7 1,312,668 - +0.4 1,312,729 + -1.9 1,312,729 + +0.7 1,312,813 - -0.2 1,312,830 - +0.1 1,312,864 + -1.3 1,313,176 - mazG Shewana3_1114 0.31 +1.5 1,313,265 - mazG Shewana3_1114 0.41 -0.1 1,313,457 + mazG Shewana3_1114 0.63 -0.5 1,313,457 + mazG Shewana3_1114 0.63 +1.3 1,313,611 + mazG Shewana3_1114 0.81 -0.8 1,313,725 + +0.7 1,313,733 - -1.0 1,313,733 - +0.5 1,313,755 + +0.1 1,313,755 + +0.1
Or see this region's nucleotide sequence