Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mdoD and Shewana3_0890 are separated by 87 nucleotides Shewana3_0890 and Shewana3_0891 are separated by 20 nucleotides Shewana3_0891 and Shewana3_0892 are separated by 247 nucleotides
Shewana3_0889: mdoD - glucan biosynthesis protein D (RefSeq), at 1,034,901 to 1,036,577
mdoD
Shewana3_0890: Shewana3_0890 - glutamine amidotransferase (RefSeq), at 1,036,665 to 1,037,381
_0890
Shewana3_0891: Shewana3_0891 - AraC family transcriptional regulator (RefSeq), at 1,037,402 to 1,038,280
_0891
Shewana3_0892: Shewana3_0892 - phosphate transporter (RefSeq), at 1,038,528 to 1,039,997
_0892
Position (kb)
1037
1038
1039 Strain fitness (log2 ratio)
-2
-1
0
1 at 1036.419 kb on + strand at 1036.505 kb on + strand at 1036.579 kb on + strand at 1036.656 kb on + strand at 1036.664 kb on - strand at 1036.751 kb on + strand, within Shewana3_0890 at 1036.995 kb on - strand, within Shewana3_0890 at 1037.413 kb on - strand at 1037.578 kb on + strand, within Shewana3_0891 at 1037.617 kb on + strand, within Shewana3_0891 at 1037.644 kb on + strand, within Shewana3_0891 at 1037.780 kb on - strand, within Shewana3_0891 at 1038.116 kb on + strand, within Shewana3_0891 at 1038.158 kb on - strand, within Shewana3_0891 at 1038.211 kb on + strand at 1038.366 kb on + strand at 1038.565 kb on - strand at 1038.748 kb on + strand, within Shewana3_0892 at 1038.964 kb on + strand, within Shewana3_0892 at 1038.964 kb on + strand, within Shewana3_0892 at 1038.964 kb on + strand, within Shewana3_0892 at 1038.972 kb on - strand, within Shewana3_0892 at 1039.076 kb on + strand, within Shewana3_0892 at 1039.238 kb on + strand, within Shewana3_0892
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 1,036,419 + -1.3 1,036,505 + -0.7 1,036,579 + +0.8 1,036,656 + -0.6 1,036,664 - -0.9 1,036,751 + Shewana3_0890 0.12 +0.6 1,036,995 - Shewana3_0890 0.46 +0.7 1,037,413 - +0.4 1,037,578 + Shewana3_0891 0.20 +0.5 1,037,617 + Shewana3_0891 0.24 +1.1 1,037,644 + Shewana3_0891 0.28 +0.5 1,037,780 - Shewana3_0891 0.43 +0.7 1,038,116 + Shewana3_0891 0.81 -0.2 1,038,158 - Shewana3_0891 0.86 +0.6 1,038,211 + +0.2 1,038,366 + -1.9 1,038,565 - +0.6 1,038,748 + Shewana3_0892 0.15 +1.3 1,038,964 + Shewana3_0892 0.30 -2.2 1,038,964 + Shewana3_0892 0.30 +1.1 1,038,964 + Shewana3_0892 0.30 -1.9 1,038,972 - Shewana3_0892 0.30 +0.0 1,039,076 + Shewana3_0892 0.37 -1.2 1,039,238 + Shewana3_0892 0.48 +0.6
Or see this region's nucleotide sequence