Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0826 and Shewana3_0827 are separated by 149 nucleotides Shewana3_0827 and Shewana3_0828 are separated by 12 nucleotides Shewana3_0828 and Shewana3_0829 are separated by 78 nucleotides
Shewana3_0826: Shewana3_0826 - hypothetical protein (RefSeq), at 956,994 to 957,647
_0826
Shewana3_0827: Shewana3_0827 - phage shock protein A, PspA (RefSeq), at 957,797 to 958,486
_0827
Shewana3_0828: Shewana3_0828 - hypothetical protein (RefSeq), at 958,499 to 958,912
_0828
Shewana3_0829: Shewana3_0829 - spermidine synthase (RefSeq), at 958,991 to 960,724
_0829
Position (kb) 958
959 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 957.568 kb on - strand, within Shewana3_0826 at 957.653 kb on + strand at 957.661 kb on - strand at 957.663 kb on - strand at 957.663 kb on - strand at 957.881 kb on - strand, within Shewana3_0827 at 958.002 kb on - strand at 958.366 kb on - strand, within Shewana3_0827 at 958.366 kb on - strand, within Shewana3_0827 at 958.432 kb on - strand at 958.531 kb on + strand at 958.539 kb on - strand at 958.555 kb on - strand, within Shewana3_0828 at 958.562 kb on + strand, within Shewana3_0828 at 958.622 kb on - strand, within Shewana3_0828 at 959.079 kb on - strand at 959.131 kb on + strand at 959.176 kb on + strand, within Shewana3_0829 at 959.217 kb on - strand, within Shewana3_0829 at 959.249 kb on - strand, within Shewana3_0829 at 959.263 kb on + strand, within Shewana3_0829 at 959.314 kb on - strand, within Shewana3_0829 at 959.339 kb on + strand, within Shewana3_0829 at 959.349 kb on + strand, within Shewana3_0829 at 959.428 kb on + strand, within Shewana3_0829 at 959.521 kb on + strand, within Shewana3_0829 at 959.743 kb on + strand, within Shewana3_0829
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 957,568 - Shewana3_0826 0.88 -1.3 957,653 + -1.1 957,661 - +0.1 957,663 - +0.5 957,663 - -2.2 957,881 - Shewana3_0827 0.12 -2.3 958,002 - -1.2 958,366 - Shewana3_0827 0.82 -0.5 958,366 - Shewana3_0827 0.82 -0.9 958,432 - -3.8 958,531 + +2.3 958,539 - +0.4 958,555 - Shewana3_0828 0.14 +0.6 958,562 + Shewana3_0828 0.15 -0.5 958,622 - Shewana3_0828 0.30 +0.8 959,079 - +0.4 959,131 + +0.5 959,176 + Shewana3_0829 0.11 -0.1 959,217 - Shewana3_0829 0.13 +0.4 959,249 - Shewana3_0829 0.15 +0.9 959,263 + Shewana3_0829 0.16 -1.6 959,314 - Shewana3_0829 0.19 -1.6 959,339 + Shewana3_0829 0.20 +0.7 959,349 + Shewana3_0829 0.21 +0.3 959,428 + Shewana3_0829 0.25 -0.6 959,521 + Shewana3_0829 0.31 +0.5 959,743 + Shewana3_0829 0.43 +0.2
Or see this region's nucleotide sequence