Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0647 and Shewana3_0648 are separated by 607 nucleotides Shewana3_0648 and Shewana3_0649 are separated by 296 nucleotides
Shewana3_0647: Shewana3_0647 - NERD domain-containing protein (RefSeq), at 763,691 to 764,680
_0647
Shewana3_0648: Shewana3_0648 - fructose-1,6-bisphosphatase (RefSeq), at 765,288 to 766,280
_0648
Shewana3_0649: Shewana3_0649 - dipeptidyl-peptidase IV (RefSeq), at 766,577 to 768,868
_0649
Position (kb) 765
766
767 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 764.337 kb on - strand, within Shewana3_0647 at 764.340 kb on + strand, within Shewana3_0647 at 764.460 kb on - strand, within Shewana3_0647 at 764.534 kb on + strand, within Shewana3_0647 at 764.563 kb on + strand, within Shewana3_0647 at 764.593 kb on + strand at 764.593 kb on + strand at 764.601 kb on - strand at 764.643 kb on - strand at 764.768 kb on + strand at 764.776 kb on - strand at 764.830 kb on - strand at 764.873 kb on - strand at 764.962 kb on + strand at 765.086 kb on + strand at 765.299 kb on - strand at 765.564 kb on + strand, within Shewana3_0648 at 765.564 kb on + strand, within Shewana3_0648 at 765.571 kb on + strand, within Shewana3_0648 at 765.579 kb on - strand, within Shewana3_0648 at 765.628 kb on - strand, within Shewana3_0648 at 765.802 kb on - strand, within Shewana3_0648 at 765.897 kb on - strand, within Shewana3_0648 at 765.979 kb on - strand, within Shewana3_0648 at 766.193 kb on - strand at 766.265 kb on + strand at 766.273 kb on - strand at 766.412 kb on + strand at 766.412 kb on + strand at 766.412 kb on + strand at 766.450 kb on + strand at 766.552 kb on - strand at 766.712 kb on - strand at 766.712 kb on - strand at 766.880 kb on - strand, within Shewana3_0649 at 767.003 kb on + strand, within Shewana3_0649 at 767.016 kb on - strand, within Shewana3_0649
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 764,337 - Shewana3_0647 0.65 -0.0 764,340 + Shewana3_0647 0.66 -1.5 764,460 - Shewana3_0647 0.78 -0.1 764,534 + Shewana3_0647 0.85 -3.1 764,563 + Shewana3_0647 0.88 -3.3 764,593 + -0.1 764,593 + +0.6 764,601 - -1.0 764,643 - +0.6 764,768 + +0.5 764,776 - -1.7 764,830 - +0.7 764,873 - +0.3 764,962 + +0.2 765,086 + -1.1 765,299 - -0.3 765,564 + Shewana3_0648 0.28 -4.2 765,564 + Shewana3_0648 0.28 -3.6 765,571 + Shewana3_0648 0.28 -2.9 765,579 - Shewana3_0648 0.29 -2.5 765,628 - Shewana3_0648 0.34 -2.0 765,802 - Shewana3_0648 0.52 -2.2 765,897 - Shewana3_0648 0.61 -2.8 765,979 - Shewana3_0648 0.70 -3.0 766,193 - -2.8 766,265 + -3.5 766,273 - -2.7 766,412 + +0.2 766,412 + -1.2 766,412 + +0.6 766,450 + +0.2 766,552 - -1.5 766,712 - +1.1 766,712 - +0.2 766,880 - Shewana3_0649 0.13 +0.9 767,003 + Shewana3_0649 0.19 +0.7 767,016 - Shewana3_0649 0.19 -2.9
Or see this region's nucleotide sequence