Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0618 and Shewana3_0619 are separated by 120 nucleotides Shewana3_0619 and Shewana3_0620 are separated by 170 nucleotides Shewana3_0620 and Shewana3_0621 are separated by 205 nucleotides Shewana3_0621 and Shewana3_0622 are separated by 171 nucleotides
Shewana3_0618: Shewana3_0618 - Sel1 domain-containing protein (RefSeq), at 723,289 to 724,068
_0618
Shewana3_0619: Shewana3_0619 - 2'-5' RNA ligase (RefSeq), at 724,189 to 724,761
_0619
Shewana3_0620: Shewana3_0620 - diguanylate cyclase/phosphodiesterase (RefSeq), at 724,932 to 727,118
_0620
Shewana3_0621: Shewana3_0621 - hypothetical protein (RefSeq), at 727,324 to 727,881
_0621
Shewana3_0622: Shewana3_0622 - ATP-dependent helicase HrpB (RefSeq), at 728,053 to 730,560
_0622
Position (kb) 724
725
726
727
728 Strain fitness (log2 ratio) -2
-1
0
1 at 724.180 kb on + strand at 724.266 kb on + strand, within Shewana3_0619 at 724.266 kb on + strand, within Shewana3_0619 at 724.291 kb on + strand, within Shewana3_0619 at 724.326 kb on + strand, within Shewana3_0619 at 724.334 kb on - strand, within Shewana3_0619 at 724.334 kb on - strand, within Shewana3_0619 at 724.441 kb on - strand, within Shewana3_0619 at 724.517 kb on - strand, within Shewana3_0619 at 724.724 kb on - strand at 724.738 kb on + strand at 725.041 kb on - strand at 725.088 kb on + strand at 725.089 kb on + strand at 725.091 kb on - strand at 725.203 kb on + strand, within Shewana3_0620 at 725.211 kb on - strand, within Shewana3_0620 at 725.211 kb on - strand, within Shewana3_0620 at 725.229 kb on + strand, within Shewana3_0620 at 725.249 kb on + strand, within Shewana3_0620 at 725.356 kb on + strand, within Shewana3_0620 at 725.433 kb on + strand, within Shewana3_0620 at 725.457 kb on + strand, within Shewana3_0620 at 725.502 kb on + strand, within Shewana3_0620 at 725.502 kb on + strand, within Shewana3_0620 at 725.510 kb on - strand, within Shewana3_0620 at 725.619 kb on + strand, within Shewana3_0620 at 725.678 kb on - strand, within Shewana3_0620 at 725.678 kb on - strand, within Shewana3_0620 at 725.693 kb on - strand, within Shewana3_0620 at 725.769 kb on - strand, within Shewana3_0620 at 725.847 kb on + strand, within Shewana3_0620 at 726.118 kb on - strand, within Shewana3_0620 at 726.235 kb on - strand, within Shewana3_0620 at 726.252 kb on + strand, within Shewana3_0620 at 726.275 kb on + strand, within Shewana3_0620 at 726.283 kb on - strand, within Shewana3_0620 at 726.297 kb on + strand, within Shewana3_0620 at 726.307 kb on - strand, within Shewana3_0620 at 726.468 kb on + strand, within Shewana3_0620 at 726.530 kb on - strand, within Shewana3_0620 at 726.547 kb on + strand, within Shewana3_0620 at 726.547 kb on + strand, within Shewana3_0620 at 726.672 kb on + strand, within Shewana3_0620 at 726.721 kb on - strand, within Shewana3_0620 at 726.929 kb on - strand at 726.967 kb on + strand at 727.071 kb on - strand at 727.087 kb on - strand at 727.088 kb on - strand at 727.088 kb on - strand at 727.095 kb on - strand at 727.410 kb on - strand, within Shewana3_0621 at 727.504 kb on - strand, within Shewana3_0621 at 727.627 kb on + strand, within Shewana3_0621 at 727.637 kb on - strand, within Shewana3_0621 at 727.659 kb on - strand, within Shewana3_0621 at 727.814 kb on - strand, within Shewana3_0621 at 727.864 kb on - strand at 727.999 kb on + strand at 727.999 kb on + strand at 728.007 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 724,180 + +0.8 724,266 + Shewana3_0619 0.13 +0.2 724,266 + Shewana3_0619 0.13 -2.1 724,291 + Shewana3_0619 0.18 -1.3 724,326 + Shewana3_0619 0.24 +1.0 724,334 - Shewana3_0619 0.25 -1.6 724,334 - Shewana3_0619 0.25 +0.3 724,441 - Shewana3_0619 0.44 -1.7 724,517 - Shewana3_0619 0.57 -1.8 724,724 - -0.2 724,738 + +0.5 725,041 - -1.5 725,088 + +0.1 725,089 + +1.3 725,091 - +0.2 725,203 + Shewana3_0620 0.12 +0.8 725,211 - Shewana3_0620 0.13 -0.4 725,211 - Shewana3_0620 0.13 +0.9 725,229 + Shewana3_0620 0.14 +0.3 725,249 + Shewana3_0620 0.14 -1.4 725,356 + Shewana3_0620 0.19 +0.0 725,433 + Shewana3_0620 0.23 +0.8 725,457 + Shewana3_0620 0.24 +0.7 725,502 + Shewana3_0620 0.26 -0.3 725,502 + Shewana3_0620 0.26 +1.7 725,510 - Shewana3_0620 0.26 -2.0 725,619 + Shewana3_0620 0.31 -0.0 725,678 - Shewana3_0620 0.34 -1.3 725,678 - Shewana3_0620 0.34 -1.4 725,693 - Shewana3_0620 0.35 -1.5 725,769 - Shewana3_0620 0.38 +0.6 725,847 + Shewana3_0620 0.42 +0.4 726,118 - Shewana3_0620 0.54 +0.8 726,235 - Shewana3_0620 0.60 -1.6 726,252 + Shewana3_0620 0.60 -0.6 726,275 + Shewana3_0620 0.61 -1.5 726,283 - Shewana3_0620 0.62 -0.3 726,297 + Shewana3_0620 0.62 +0.0 726,307 - Shewana3_0620 0.63 -0.1 726,468 + Shewana3_0620 0.70 +0.5 726,530 - Shewana3_0620 0.73 +0.3 726,547 + Shewana3_0620 0.74 -1.9 726,547 + Shewana3_0620 0.74 -1.2 726,672 + Shewana3_0620 0.80 -1.2 726,721 - Shewana3_0620 0.82 -1.2 726,929 - -2.1 726,967 + -1.4 727,071 - +0.8 727,087 - -1.2 727,088 - +0.6 727,088 - -1.1 727,095 - +0.2 727,410 - Shewana3_0621 0.15 +0.4 727,504 - Shewana3_0621 0.32 -1.1 727,627 + Shewana3_0621 0.54 +0.4 727,637 - Shewana3_0621 0.56 -1.2 727,659 - Shewana3_0621 0.60 -1.0 727,814 - Shewana3_0621 0.88 +1.0 727,864 - -1.2 727,999 + -2.7 727,999 + -0.7 728,007 - -1.4
Or see this region's nucleotide sequence