Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0516 and Shewana3_0517 are separated by 15 nucleotides Shewana3_0517 and Shewana3_0518 are separated by 77 nucleotides
Shewana3_0516: Shewana3_0516 - outer membrane efflux family protein, putative (RefSeq), at 604,751 to 606,121
_0516
Shewana3_0517: Shewana3_0517 - RND family efflux transporter MFP subunit (RefSeq), at 606,137 to 607,417
_0517
Shewana3_0518: Shewana3_0518 - CzcA family heavy metal efflux protein (RefSeq), at 607,495 to 610,707
_0518
Position (kb) 606
607
608 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 605.159 kb on - strand, within Shewana3_0516 at 605.224 kb on + strand, within Shewana3_0516 at 605.234 kb on - strand, within Shewana3_0516 at 605.276 kb on - strand, within Shewana3_0516 at 605.295 kb on + strand, within Shewana3_0516 at 605.303 kb on + strand, within Shewana3_0516 at 605.305 kb on + strand, within Shewana3_0516 at 605.311 kb on - strand, within Shewana3_0516 at 605.326 kb on - strand, within Shewana3_0516 at 605.593 kb on + strand, within Shewana3_0516 at 605.601 kb on - strand, within Shewana3_0516 at 605.628 kb on + strand, within Shewana3_0516 at 605.630 kb on - strand, within Shewana3_0516 at 605.669 kb on - strand, within Shewana3_0516 at 605.669 kb on - strand, within Shewana3_0516 at 605.712 kb on - strand, within Shewana3_0516 at 606.035 kb on - strand at 606.135 kb on - strand at 606.194 kb on + strand at 606.233 kb on + strand at 606.249 kb on + strand at 606.249 kb on + strand at 606.303 kb on - strand, within Shewana3_0517 at 606.442 kb on - strand, within Shewana3_0517 at 606.490 kb on + strand, within Shewana3_0517 at 606.661 kb on + strand, within Shewana3_0517 at 606.727 kb on - strand, within Shewana3_0517 at 606.884 kb on + strand, within Shewana3_0517 at 606.913 kb on + strand, within Shewana3_0517 at 607.031 kb on + strand, within Shewana3_0517 at 607.075 kb on + strand, within Shewana3_0517 at 607.101 kb on - strand, within Shewana3_0517 at 607.144 kb on - strand, within Shewana3_0517 at 607.277 kb on - strand, within Shewana3_0517 at 607.332 kb on - strand at 607.384 kb on + strand at 607.384 kb on + strand at 607.392 kb on - strand at 607.392 kb on - strand at 607.392 kb on - strand at 607.392 kb on - strand at 607.640 kb on + strand at 607.659 kb on - strand at 607.711 kb on + strand at 607.745 kb on + strand at 607.783 kb on - strand at 607.865 kb on + strand, within Shewana3_0518 at 607.895 kb on + strand, within Shewana3_0518 at 608.001 kb on + strand, within Shewana3_0518 at 608.091 kb on + strand, within Shewana3_0518 at 608.230 kb on - strand, within Shewana3_0518 at 608.268 kb on + strand, within Shewana3_0518 at 608.291 kb on + strand, within Shewana3_0518 at 608.301 kb on + strand, within Shewana3_0518
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 605,159 - Shewana3_0516 0.30 -3.2 605,224 + Shewana3_0516 0.35 +0.7 605,234 - Shewana3_0516 0.35 -1.7 605,276 - Shewana3_0516 0.38 +0.4 605,295 + Shewana3_0516 0.40 +0.0 605,303 + Shewana3_0516 0.40 +0.3 605,305 + Shewana3_0516 0.40 -0.6 605,311 - Shewana3_0516 0.41 -1.8 605,326 - Shewana3_0516 0.42 -1.4 605,593 + Shewana3_0516 0.61 +0.4 605,601 - Shewana3_0516 0.62 -1.5 605,628 + Shewana3_0516 0.64 -1.6 605,630 - Shewana3_0516 0.64 +0.1 605,669 - Shewana3_0516 0.67 -1.6 605,669 - Shewana3_0516 0.67 +0.3 605,712 - Shewana3_0516 0.70 -1.8 606,035 - -2.2 606,135 - +0.3 606,194 + +0.4 606,233 + +0.6 606,249 + +0.8 606,249 + +0.3 606,303 - Shewana3_0517 0.13 -0.1 606,442 - Shewana3_0517 0.24 +0.6 606,490 + Shewana3_0517 0.28 +1.1 606,661 + Shewana3_0517 0.41 +0.7 606,727 - Shewana3_0517 0.46 -1.1 606,884 + Shewana3_0517 0.58 -0.5 606,913 + Shewana3_0517 0.61 +0.3 607,031 + Shewana3_0517 0.70 +1.5 607,075 + Shewana3_0517 0.73 -1.0 607,101 - Shewana3_0517 0.75 -2.2 607,144 - Shewana3_0517 0.79 +0.3 607,277 - Shewana3_0517 0.89 -2.0 607,332 - +0.1 607,384 + -0.7 607,384 + +0.1 607,392 - +0.5 607,392 - +0.2 607,392 - +0.6 607,392 - -1.7 607,640 + -1.5 607,659 - -1.3 607,711 + +0.4 607,745 + +0.6 607,783 - -1.5 607,865 + Shewana3_0518 0.12 +0.6 607,895 + Shewana3_0518 0.12 -2.3 608,001 + Shewana3_0518 0.16 -1.2 608,091 + Shewana3_0518 0.19 +0.9 608,230 - Shewana3_0518 0.23 -1.7 608,268 + Shewana3_0518 0.24 +0.1 608,291 + Shewana3_0518 0.25 +0.1 608,301 + Shewana3_0518 0.25 +0.2
Or see this region's nucleotide sequence