Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0390 and dapF are separated by 70 nucleotides dapF and Shewana3_0392 overlap by 4 nucleotides Shewana3_0392 and Shewana3_0393 are separated by 48 nucleotides
Shewana3_0390: Shewana3_0390 - diaminopimelate decarboxylase (RefSeq), at 450,725 to 451,969
_0390
Shewana3_0391: dapF - diaminopimelate epimerase (RefSeq), at 452,040 to 452,867
dapF
Shewana3_0392: Shewana3_0392 - hypothetical protein (RefSeq), at 452,864 to 453,499
_0392
Shewana3_0393: Shewana3_0393 - tyrosine recombinase XerC (RefSeq), at 453,548 to 454,447
_0393
Position (kb) 452
453 Strain fitness (log2 ratio) -7
-6
-5
-4
-3
-2
-1
0
1 at 451.123 kb on - strand, within Shewana3_0390 at 451.218 kb on + strand, within Shewana3_0390 at 451.226 kb on - strand, within Shewana3_0390 at 451.226 kb on - strand, within Shewana3_0390 at 451.249 kb on + strand, within Shewana3_0390 at 451.273 kb on - strand, within Shewana3_0390 at 451.300 kb on + strand, within Shewana3_0390 at 451.326 kb on + strand, within Shewana3_0390 at 451.326 kb on + strand, within Shewana3_0390 at 451.326 kb on + strand, within Shewana3_0390 at 451.440 kb on + strand, within Shewana3_0390 at 451.491 kb on + strand, within Shewana3_0390 at 451.669 kb on + strand, within Shewana3_0390 at 451.677 kb on - strand, within Shewana3_0390 at 451.795 kb on + strand, within Shewana3_0390 at 451.863 kb on - strand at 451.911 kb on - strand at 451.939 kb on + strand at 452.873 kb on - strand at 452.968 kb on + strand, within Shewana3_0392 at 453.232 kb on - strand, within Shewana3_0392 at 453.458 kb on + strand at 453.485 kb on - strand at 453.493 kb on + strand at 453.501 kb on - strand at 453.501 kb on - strand at 453.502 kb on - strand at 453.686 kb on - strand, within Shewana3_0393 at 453.751 kb on - strand, within Shewana3_0393 at 453.860 kb on - strand, within Shewana3_0393
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 451,123 - Shewana3_0390 0.32 -4.8 451,218 + Shewana3_0390 0.40 -4.6 451,226 - Shewana3_0390 0.40 -5.0 451,226 - Shewana3_0390 0.40 -5.5 451,249 + Shewana3_0390 0.42 -5.4 451,273 - Shewana3_0390 0.44 -5.6 451,300 + Shewana3_0390 0.46 -4.4 451,326 + Shewana3_0390 0.48 -7.7 451,326 + Shewana3_0390 0.48 -3.5 451,326 + Shewana3_0390 0.48 -5.1 451,440 + Shewana3_0390 0.57 -6.1 451,491 + Shewana3_0390 0.62 -6.1 451,669 + Shewana3_0390 0.76 -5.2 451,677 - Shewana3_0390 0.76 -5.9 451,795 + Shewana3_0390 0.86 -3.0 451,863 - -4.2 451,911 - -5.2 451,939 + -6.7 452,873 - -1.5 452,968 + Shewana3_0392 0.16 -0.7 453,232 - Shewana3_0392 0.58 -1.1 453,458 + -0.3 453,485 - -1.0 453,493 + -4.8 453,501 - +0.0 453,501 - +1.0 453,502 - -1.2 453,686 - Shewana3_0393 0.15 -0.7 453,751 - Shewana3_0393 0.23 -4.4 453,860 - Shewana3_0393 0.35 -0.8
Or see this region's nucleotide sequence