Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0318 and Shewana3_0319 are separated by 95 nucleotides Shewana3_0319 and Shewana3_0320 are separated by 126 nucleotides Shewana3_0320 and Shewana3_0321 are separated by 57 nucleotides Shewana3_0321 and Shewana3_0322 are separated by 349 nucleotides
Shewana3_0318: Shewana3_0318 - rhodanese domain-containing protein (RefSeq), at 353,816 to 354,139
_0318
Shewana3_0319: Shewana3_0319 - XRE family transcriptional regulator (RefSeq), at 354,235 to 354,810
_0319
Shewana3_0320: Shewana3_0320 - benzoate transporter (RefSeq), at 354,937 to 356,127
_0320
Shewana3_0321: Shewana3_0321 - GCN5-related N-acetyltransferase (RefSeq), at 356,185 to 356,640
_0321
Shewana3_0322: Shewana3_0322 - antibiotic acetyltransferase (RefSeq), at 356,990 to 357,637
_0322
Position (kb) 354
355
356
357 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 353.943 kb on - strand, within Shewana3_0318 at 354.137 kb on - strand at 354.220 kb on + strand at 354.354 kb on + strand, within Shewana3_0319 at 354.362 kb on - strand, within Shewana3_0319 at 354.426 kb on + strand, within Shewana3_0319 at 354.657 kb on + strand, within Shewana3_0319 at 354.665 kb on - strand, within Shewana3_0319 at 354.680 kb on - strand, within Shewana3_0319 at 354.712 kb on + strand, within Shewana3_0319 at 354.745 kb on + strand, within Shewana3_0319 at 354.753 kb on + strand at 355.093 kb on - strand, within Shewana3_0320 at 355.097 kb on + strand, within Shewana3_0320 at 355.158 kb on + strand, within Shewana3_0320 at 355.282 kb on - strand, within Shewana3_0320 at 355.357 kb on - strand, within Shewana3_0320 at 355.359 kb on + strand, within Shewana3_0320 at 355.401 kb on - strand, within Shewana3_0320 at 355.424 kb on + strand, within Shewana3_0320 at 355.428 kb on - strand, within Shewana3_0320 at 355.450 kb on - strand, within Shewana3_0320 at 355.536 kb on + strand, within Shewana3_0320 at 355.587 kb on + strand, within Shewana3_0320 at 355.602 kb on + strand, within Shewana3_0320 at 355.775 kb on - strand, within Shewana3_0320 at 355.843 kb on + strand, within Shewana3_0320 at 355.991 kb on - strand, within Shewana3_0320 at 356.150 kb on - strand at 356.173 kb on - strand at 356.183 kb on - strand at 356.183 kb on - strand at 356.282 kb on - strand, within Shewana3_0321 at 356.370 kb on + strand, within Shewana3_0321 at 356.482 kb on + strand, within Shewana3_0321 at 356.500 kb on - strand, within Shewana3_0321 at 356.503 kb on + strand, within Shewana3_0321 at 356.529 kb on + strand, within Shewana3_0321 at 356.613 kb on + strand at 356.737 kb on + strand at 356.765 kb on + strand at 356.831 kb on - strand at 356.877 kb on - strand at 356.903 kb on + strand at 356.924 kb on + strand at 357.123 kb on + strand, within Shewana3_0322
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 353,943 - Shewana3_0318 0.39 +0.3 354,137 - -2.3 354,220 + -1.4 354,354 + Shewana3_0319 0.21 -1.9 354,362 - Shewana3_0319 0.22 -3.6 354,426 + Shewana3_0319 0.33 -3.2 354,657 + Shewana3_0319 0.73 -5.3 354,665 - Shewana3_0319 0.75 -4.3 354,680 - Shewana3_0319 0.77 -3.5 354,712 + Shewana3_0319 0.83 -3.8 354,745 + Shewana3_0319 0.89 -4.0 354,753 + -4.1 355,093 - Shewana3_0320 0.13 -1.6 355,097 + Shewana3_0320 0.13 +0.7 355,158 + Shewana3_0320 0.19 +0.1 355,282 - Shewana3_0320 0.29 -0.5 355,357 - Shewana3_0320 0.35 +0.9 355,359 + Shewana3_0320 0.35 +0.7 355,401 - Shewana3_0320 0.39 -1.2 355,424 + Shewana3_0320 0.41 -0.6 355,428 - Shewana3_0320 0.41 +0.5 355,450 - Shewana3_0320 0.43 -0.0 355,536 + Shewana3_0320 0.50 +0.3 355,587 + Shewana3_0320 0.55 -2.9 355,602 + Shewana3_0320 0.56 +0.5 355,775 - Shewana3_0320 0.70 +0.2 355,843 + Shewana3_0320 0.76 -0.1 355,991 - Shewana3_0320 0.88 +0.2 356,150 - +0.3 356,173 - +1.0 356,183 - -1.4 356,183 - -3.0 356,282 - Shewana3_0321 0.21 +0.4 356,370 + Shewana3_0321 0.41 -1.5 356,482 + Shewana3_0321 0.65 -1.5 356,500 - Shewana3_0321 0.69 +1.0 356,503 + Shewana3_0321 0.70 +0.4 356,529 + Shewana3_0321 0.75 +0.3 356,613 + -1.1 356,737 + +0.6 356,765 + -1.3 356,831 - +0.9 356,877 - +0.8 356,903 + -1.1 356,924 + +0.3 357,123 + Shewana3_0322 0.21 +0.5
Or see this region's nucleotide sequence